PERAMIVIR AND RELATED IMPURITIES IN RAT PLASMA AND ITS APPLICATIONS IN PHARMACOKINETIC STUDIES (BIOANALYTICAL METHOD DEVELOPMENT AND VALIDATION BY LC-MS/MS)

Authors

  • THULASEEDHAR ALUMURI Department of Chemistry, GITAM (Deemed to be University), Bengaluru 560034, Karnataka, India https://orcid.org/0000-0003-3841-0470
  • KARUNASREE MERUGU Department of Chemistry, GITAM (Deemed to be University), Bengaluru 560034, Karnataka, India
  • NAMBURI L. A. AMARABABU New Generation Materials Lab (NGML), Department of Science and Humanities, Vignan’s Foundation for Science Technology and Research University (VFSTR) (Deemed to be University), Vadlamudi, Guntur 522213, Andhra Pradesh, India
  • ARAVIND KURNOOL Department of Chemistry, Osmania University, Hyderabad 500007, Telangana, India

DOI:

https://doi.org/10.22159/ijap.2022v14i5.45457

Keywords:

Peramivir, LC-MS/MS, Development, Validation, Rat plasma

Abstract

Objective: New LC-MS/MS method for the estimation of Peramivir and its associated substances were developed and validated

Methods: Optimized (Developed) method includes gradient elution of peramivir and its related substances with a flow of 1 ml/min and waters X-bridge C18 column of dimensions 150mmx4.6mm, 3.5µ. 0.1% formic acid and acetonitrile were used as mobile phase. Sarilumab was used as an internal standard. 40 min run time was used to separate peramivir and its related substances.

Results: The calibration curve was linear in the concentration percentage range from 10%-200% of Peramivir and its related substances. The calibration charts plotted were linear with a regression coefficient of R2 > 0.999. Accuracy, precision, recovery, matrix effect and stability results were found to be within the suitable limits. A Simple and efficient method was developed and utilized in pharmacokinetic studies to see the investigated analyte in body fluids.

Conclusion: This application denotes all parameters such as accuracy, precision, recovery, stability etc which are in good agreement with the USFDA guidelines and are effectively applied to the investigation of the pharmacokinetic studies in rat plasma.

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Published

19-07-2022

How to Cite

ALUMURI, T., MERUGU, K., AMARABABU, N. L. A., & KURNOOL, A. (2022). PERAMIVIR AND RELATED IMPURITIES IN RAT PLASMA AND ITS APPLICATIONS IN PHARMACOKINETIC STUDIES (BIOANALYTICAL METHOD DEVELOPMENT AND VALIDATION BY LC-MS/MS). International Journal of Applied Pharmaceutics, 14(5). https://doi.org/10.22159/ijap.2022v14i5.45457

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