• DEVASENA K M Dept. of Rasashastra and Bhaishajyakalpana(Pharmaceuticals), Amrita School of Ayurveda, Amrita Vishwavidyapeetham, Amritapuri, Vallikkavu, Kollam-690525, Kerala, India
  • LEKSHMI DEVI S. Dept. of Rasashastra and Bhaishajyakalpana(Pharmaceuticals), Amrita School of Ayurveda, Amrita Vishwavidyapeetham, Amritapuri, Vallikkavu, Kollam-690525, Kerala, India
  • ARUN MOHANAN Dept. of Rasashastra and Bhaishajyakalpana(Pharmaceuticals), Amrita School of Ayurveda, Amrita Vishwavidyapeetham, Amritapuri, Vallikkavu, Kollam-690525, Kerala, India
  • RAMESH N. V. Dept. of Rasashastra and Bhaishajyakalpana(Pharmaceuticals), Amrita School of Ayurveda, Amrita Vishwavidyapeetham, Amritapuri, Vallikkavu, Kollam-690525, Kerala, India


Triphala, Terminalia chebula, Terminalia bellerica, Phyllanthus embilica, anticancer, molecular mechanism, bioactive compound



Ayurveda is a traditional Indian system of medicine which has been practiced for 3000 years. It gives various single and combinations of herbal and mineral drugs for various conditions. Novel technologies and scientific approaches can make use of such traditional knowledge and help develop new drugs and/or therapeutic uses as well as give evidence to certain traditional practices.


This review is based on Ayurvedic texts and scientific online databases like PubMed, GoogleScholar, ScienceDirect to study use of scientific research methods in ayurvedic formulation Triphala.


The following keywords were used: Triphala, terminalia chebula, terminalia bellerica, Phyllanthus embilica, chemical constituents, gallic acid, chebulinic acid, molecular docking. Studies about Triphala and their individual drugs as well as their active compounds were concentrated upon. There are many research works being conducted on Triphala and its therapeutic effect. Yet, the need to develop these works to a fully utilisable form for medical community is not achieved.


This review concludes that Triphala is a treasure that needs to be further evaluated for betterment of health globally.


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Yuan H, Ma Q, Ye L, Piao G. The Traditional Medicine and Modern Medicine from Natural Products. Molecules. 2016 Apr 29;21(5):559. doi: 10.3390/molecules21050559. PMID: 27136524; PMCID: PMC6273146.

Puranik A, Patwardhan B. Ayurveda and Metabolic Diseases: The Whole is Greater than the Sum of the Parts. Nutritional and Therapeutic Interventions for Diabetes and Metabolic Syndrome. 2012; 443–456.

Shastri PP. Sharangadhara Samhita of Sharangadhara, Sanskrit text with Adhamalla’s Dipika Commentary and Kashiram’s Gudartha-Dipika Commentary, Madhyamakhanda, Ch. 6. Ver.9-11;179.

Sharma PV. Charaka Samhita Part 2, Chikitsasthana 1.3/41-42. Chaukambha Orientalia Varanasi. 2011;24.

Vahata, Sarvakshirogapratishedham Namadhyayam in Ashtavaidyan Vaidyamadhom Cheriya Narayanan Namboodiri MathAshtanga Hridaya Samhitha with Sasilekha commentary of Indu, Varanasi, India, Chowkambha Krishnadas Academy, Second Edition:2019;513.

Belapurkar P, Goyal P, Tiwari-Barua P. Immunomodulatory effects of triphala and its individual constituents: a review. Indian J Pharm Sci. 2014 Nov-Dec;76(6):467-75. PMID: 25593379; PMCID: PMC4293677.

Peterson CT, Pourang A, Dhaliwal S, Kohn JN, Uchitel S, Singh H, Mills PJ, Peterson SN, Sivamani RK. Modulatory Effects of Triphala and Manjistha Dietary Supplementation on Human Gut Microbiota: A Double-Blind, Randomized, Placebo-Controlled Pilot Study. J Altern Complement Med. 2020 Nov;26(11):1015-1024. doi: 10.1089/acm.2020.0148. Epub 2020 Sep 18. PMID: 32955913.

Wang W, Liu T, Yang L, Ma Y, Dou F, Shi L, Wen A, Ding Y. Study on the multi-targets mechanism of triphala on cardio-cerebral vascular diseases based on network pharmacology. Biomed Pharmacother. 2019 Aug;116:108994. doi: 10.1016/j.biopha.2019.108994. Epub 2019 May 18. PMID: 31112872.

Shi Y, Sahu RP, Srivastava SK. Triphala inhibits both in vitro and in vivo xenograft growth of pancreatic tumor cells by inducing apoptosis. BMC Cancer. 2008 Oct 10;8:294. doi: 10.1186/1471-2407-8-294. PMID: 18847491; PMCID: PMC2576337.

Nariya M, Shukla V, Jain S, Ravishankar B. Comparison of enteroprotective efficacy of triphala formulations (Indian Herbal Drug) on methotrexate-induced small intestinal damage in rats. Phytother Res. 2009 Aug;23(8):1092-8. doi: 10.1002/ptr.2744. PMID: 19170156.

Mashyal P, Bhargav H, Raghuram N. Safety and usefulness of Laghu shankha prakshalana in patients with essential hypertension: A self controlled clinical study. J Ayurveda Integr Med. 2014 Oct-Dec;5(4):227-35. doi: 10.4103/0975-9476.131724. PMID: 25624697; PMCID: PMC4296435.

Rudrapal M, Celik I, Khan J, Ansari MA, Alomary MN, Yadav R, Sharma T, Tallei TE, Pasala PK, Sahoo RK, Khairnar SJ, Bendale AR, Zothantluanga JH, Chetia D, Walode SG. Identification of bioactive molecules from Triphala (Ayurvedic herbal formulation) as potential inhibitors of SARS-CoV-2 main protease (Mpro) through computational investigations. J King Saud Univ Sci. 2022 Apr;34(3):101826. doi: 10.1016/j.jksus.2022.101826. Epub 2022 Jan 10. PMID: 35035181; PMCID: PMC8744360.

Jagetia GC, Baliga MS, Malagi KJ, Sethukumar Kamath M. The evaluation of the radioprotective effect of Triphala (an ayurvedic rejuvenating drug) in the mice exposed to gamma-radiation. Phytomedicine. 2002 Mar;9(2):99-108. doi: 10.1078/0944-7113-00095. PMID: 11995956.

Mohanasundaram.S, N.Rangarajan, V.Sampath, K.Porkodi, M.Pennarasi. GC-MS and HPLC analysis of Antiglycogenolytic and Glycogenic compounds in Kaempferol 3 – O – gentiobioside containing Senna alata L leaves in experimental rats. Translational Metabolic Syndrome Research., 2021; 4:10-17.

Vemuri P. K, Dronavalli L, Nayakudugari P, Kunta A, Challagulla R. Phytochemical Analysis and Biochemical Characterization of Terminalia Chebula Extracts For Its Medicinal Use. Biomed Pharmacol J. 2019;12(3).

Wu L, Qiunan Z, Wenyi L, Yongben M, Liying N, Lanzhen Z. Phytochemical Analysis Using UPLC-MSn Combined with Network Pharmacology Approaches to Explore the Biomarkers for the Quality Control of the Anticancer Tannin Fraction of Phyllanthus emblica L. Habitat in Nepal. Evidence-Based Complementary and Alternative Medicine. vol. 2021, Article ID 6623791, 19 pages, 2021.

Li K, Han X, Li R, Xu Z, Pan T, Liu J, Li B, Wang S, Diao Y, Liu X. Composition, Antivirulence Activity, and Active Property Distribution of the Fruit of Terminalia chebula Retz. J Food Sci. 2019 Jul;84(7):1721-1729. doi: 10.1111/1750-3841.14655. Epub 2019 Jun 17. PMID: 31206192.

Zhang XR, Zhu HT, Wang D, Yang Z, Yang CR, Zhang YJ. Termitomenins A-E: Five new lignans from Terminalia chebula var. tomentella (Kurz) C. B. Clarke. Fitoterapia. 2020 Jun;143:104571. doi: 10.1016/j.fitote.2020.104571. Epub 2020 Mar 21. PMID: 32209392.

Kumar N, Khurana SM. Phytochemistry and medicinal potential of the Terminalia bellirica Roxb. ( Bahera ). Indian Journal of Natural Products and Resources. 2018;9(2): 97-107.

Makihara H, Koike Y, Ohta M, Horiguchi-Babamoto E, Tsubata M, Kinoshita K, Akase T, Goshima Y, Aburada M, Shimada T. Gallic Acid, the Active Ingredient of Terminalia bellirica, Enhances Adipocyte Differentiation and Adiponectin Secretion. Biol Pharm Bull. 2016;39(7):1137-43. doi: 10.1248/bpb.b16-00064. PMID: 27374289.

Yang F, Yaseen A, Chen B, Li F, Wang L, Hu W, Wang M. Chemical constituents from the fruits of Phyllanthus emblica L.. Biochemical Systematics and Ecology. 2020; 92: 104122–. doi:10.1016/j.bse.2020.104122

Pawar V, Lahorkar P, Anantha Narayana DB. Development of a RP-HPLC Method for Analysis of Triphala Curna and its Applicability to Test Variations in Triphala Curna Preparations. Indian J Pharm Sci. 2009 Jul;71(4):382-6. doi: 10.4103/0250-474X.57286. PMID: 20502543; PMCID: PMC2865809.

Mohanasundaram S, Victor Arokia Doss, Prasad Maddisetty, Magesh R, Sivakumar K and Subathra M. Pharmacological analysis of hydroethanolic extract of Senna alata (L.) for in vitro free radical scavenging and cytotoxic activities against HepG2 cancer cell line. Pak. J. Pharm. Sci., 2019; 32(3):931-934.

Pandurangan AK, Mohebali N, Esa NM, Looi CY, Ismail S, Saadatdoust Z. Gallic acid suppresses inflammation in dextran sodium sulfate-induced colitis in mice: Possible mechanisms. Int Immunopharmacol. 2015 Oct;28(2):1034-43. doi: 10.1016/j.intimp.2015.08.019. Epub 2015 Aug 28. PMID: 26319951.

Li Y, Zhang L, Wang X, Wu W, Qin R. Effect of Syringic acid on antioxidant biomarkers and associated inflammatory markers in mice model of asthma. Drug Dev Res. 2019 Mar;80(2):253-261. doi: 10.1002/ddr.21487. Epub 2018 Nov 25. PMID: 30474283.

Lin Y, Luo T, Weng A, Huang X, Yao Y, Fu Z, Li Y, Liu A, Li X, Chen D, Pan H. Gallic Acid Alleviates Gouty Arthritis by Inhibiting NLRP3 Inflammasome Activation and Pyroptosis Through Enhancing Nrf2 Signaling. Front Immunol. 2020 Dec 7;11:580593. doi: 10.3389/fimmu.2020.580593. PMID: 33365024; PMCID: PMC7750458.

Variya BC, Bakrania AK, Madan P, Patel SS. Acute and 28-days repeated dose sub-acute toxicity study of gallic acid in albino mice. Regul Toxicol Pharmacol. 2019 Feb;101:71-78. doi: 10.1016/j.yrtph.2018.11.010. Epub 2018 Nov 20. PMID: 30465803.

Lu K, Basu S. The natural compound chebulagic acid inhibits vascular endothelial growth factor A mediated regulation of endothelial cell functions. Sci Rep. 2015 Apr 10;5:9642. doi: 10.1038/srep09642. PMID: 25859636; PMCID: PMC4819393.

Vasu G, Sundaram R, Muthu K. Chebulagic acid attenuates HFD/streptozotocin induced impaired glucose metabolism and insulin resistance via up regulations of PPAR γ and GLUT 4 in type 2 diabetic rats. Toxicol Mech Methods. 2022 Mar;32(3):159-170. doi: 10.1080/15376516.2021.1976333. Epub 2021 Sep 22. PMID: 34470562.

Shanmuganathan S, Angayarkanni N. Chebulagic acid Chebulinic acid and Gallic acid, the active principles of Triphala, inhibit TNFα induced pro-angiogenic and pro-inflammatory activities in retinal capillary endothelial cells by inhibiting p38, ERK and NFkB phosphorylation. Vascul Pharmacol. 2018 Sep;108:23-35. doi: 10.1016/j.vph.2018.04.005. Epub 2018 Apr 17. PMID: 29678603.

Yang Y, Xiu J, Liu J, Zhang L, Li X, Xu Y, Qin C, Zhang L. Chebulagic acid, a hydrolyzable tannin, exhibited antiviral activity in vitro and in vivo against human enterovirus 71. Int J Mol Sci. 2013 May 3;14(5):9618-27. doi: 10.3390/ijms14059618. Erratum in: Int J Mol Sci. 2021 Aug 06;22(16): PMID: 23644889; PMCID: PMC3676802.

Mohanasundaram, S., Ramirez-Asis, E., Quispe-Talla, A. et al. Experimental replacement of hops by mango in beer: production and comparison of total phenolics, flavonoids, minerals, carbohydrates, proteins and toxic substances. Int J Syst Assur Eng Manag 2022; 13, 132–145.

Kim J, Ahn D, Chung SJ. Chebulinic Acid Suppresses Adipogenesis in 3T3-L1 Preadipocytes by Inhibiting PPP1CB Activity. Int J Mol Sci. 2022 Jan 13;23(2):865. doi: 10.3390/ijms23020865. PMID: 35055051; PMCID: PMC8775935.

Wang M, Li Y, Hu X. Chebulinic acid derived from triphala is a promising antitumour agent in human colorectal carcinoma cell lines. BMC Complement Altern Med. 2018 Dec 27;18(1):342. doi: 10.1186/s12906-018-2412-5. PMID: 30587184; PMCID: PMC6307174.

Srinivasulu C, Ramgopal M, Ramanjaneyulu G, Anuradha CM, Suresh Kumar C. Syringic acid (SA) ‒ A Review of Its Occurrence, Biosynthesis, Pharmacological and Industrial Importance. Biomed Pharmacother. 2018 Dec;108:547-557. doi: 10.1016/j.biopha.2018.09.069. Epub 2018 Sep 20. PMID: 30243088.

Mohanasundaram.S, N.Rangarajan, V.Sampath, K.Porkodi, M.V.Dass Prakash, N.Monicka. GC-MS Identification of Anti-inflammatory and Anticancer Metabolites in Edible Milky White Mushroom (Calocybe indica) against Human Breast Cancer (MCF-7) Cells. Res J Pharm and Tech., 2021; 14(8):4300-4306..

Rangarajan.N, Sampath.V, Dass Prakash MV, Mohanasundaram.S, (2021). UV Spectophotometry and FTIR analysis of Phenolic Compounds with Antioxidant Potentials in Glycyrrhiza glabra and Zingiber officinale. Int. J. Res. Pharm. Sci., 12(1):877-883.

H. Robles,Tannic Acid, Editor(s): Philip Wexler, Encyclopedia of Toxicology (Third Edition), Academic Press, 2014, Pages 474-475, ISBN 9780123864550, (

Barium sulfate, Editor(s): J.K. Aronson, Meyler's Side Effects of Drugs (Sixteenth Edition), Elsevier, 2016, Pages 827-829, ISBN 9780444537164, (

Chun JH, Henckel MM, Knaub LA, Hull SE, Pott GB, Walker LA, Reusch JE, Keller AC. (-)-Epicatechin Improves Vasoreactivity and Mitochondrial Respiration in Thermoneutral-Housed Wistar Rat Vasculature. Nutrients. 2022 Mar 5;14(5):1097. doi: 10.3390/nu14051097. PMID: 35268072; PMCID: PMC8912787.

Rangarajan.N, Sangeetha.R, Mohanasundaram.S, Sampath.V, Pokodi.K, Dass Prakash MV. Additive inhibitory effect of the peels of Citrus limon and Citrus sinensis against amylase and glucosidase activity. Int. J. Res. Pharm. Sci., 2020; 11(4), 6876-6880.

Zhang M, Vervoort L, Moalin M, Mommers A, Douny C, den Hartog GJM, Haenen GRMM. The chemical reactivity of (-)-epicatechin quinone mainly resides in its B-ring. Free Radic Biol Med. 2018 Aug 20;124:31-39. doi: 10.1016/j.freeradbiomed.2018.05.087. Epub 2018 May 31. PMID: 29859347.

Wang, Q., Sivakumar, K. & Mohanasundaram, S. Impacts of extrusion processing on food nutritional components. Int J Syst Assur Eng Manag 2022; 13, 364–374.

Chowdhury HU, Adnan MD, Oh KK, Cho DH. Decrypting molecular mechanism insight of Phyllanthus emblica L. fruit in the treatment of type 2 diabetes mellitus by network pharmacology, Phytomedicine Plus.2021;1(4):100144, ISSN 2667-0313,

Bansode, Twinkle S, Salalkar BK. Strategies in the design of antidiabetic drugs from Terminalia chebula using in silico and in vitro approach. Micromedicine. 2016; 4(2):60–67.

Rajmohamed MA, Natarajan S, Palanisamy P, Abdulkader AM, Govindaraju A. Antioxidant and cholinesterase inhibitory activities of ethyl acetate extract of Terminalia chebula: Cell-free In vitro and In silico studies. Phcog Mag. 2017;13 Suppl S3:437-4

Fei CL, Anand G, Al-Nema M. In Silico Investigations on the Probable Macromolecular Drug Targets Involved in the Anti-Schizophrenia Activity of Terminalia bellerica. Letters in Organic Chemistry. 2021; 19(1):83-92 .

Meghana Y, Srikanth J. Ex vivo, In-vitro and In-silico Neuroprotective Activity of Selected Traditional Medicinal Plants-A Reverse Pharmacological Approach. International Journal of Pharmaceutical Research. 2021.

Majumder M, Khanam T, Rahaman M, Rahimul M, Hossain TN, Chakrabarty N. Anticancer Potential of Isolated Phytochemicals From Terminalia Bellerica Against Breast Cancer: In Silico Molecular Docking Approach. World J Pharm Res. 2017 Feb 19;6(4):1763-71.

Khan SL, Siddiqui FA, Shaikh MS, Nema NV, Shaikh AA. Discovery of potential inhibitors of the receptor-binding domain (RBD) of pandemic disease-causing SARS-CoV-2 Spike glycoprotein from Triphala through molecular docking. Curr. Chinese Chem. 2021;1.

Unnisa A, Khan SL, Sheikh FAH, Mahefooz S, Kazi AA, Siddiqui FA, Gawai N, Saboo SG. In-silico Inhibitory Potential of Triphala Constituents Against Cytochrome P450 2E1 for the Prevention of Thioacetamide-induced Hepatotoxicity. J Pharm Res Int [Internet]. 2021; 367–75. Available from:

Abhinand CS, Athira PA, Soumya SJ, Sudhakaran PR. Multiple Targets Directed Multiple Ligands: An In Silico and In Vitro Approach to Evaluating the Effect of Triphala on Angiogenesis. Biomolecules. 2020 Jan 23;10(2):177. doi: 10.3390/biom10020177. PMID: 31979409; PMCID: PMC7072423.

Chandran U, Mehendale N, Patil S, Chaguturu R, Patwardhan B. Network Pharmacology. Innovative Approaches in Drug Discovery. 2017:127–64. doi: 10.1016/B978-0-12-801814-9.00005-2. Epub 2016 Oct 14. PMCID: PMC7148629.

Zhao Y, Wang M, Tsering J, Li H, Li S, Li Y, Liu Y, Hu X. An integrated study on the antitumor effect and mechanism of Triphala against gynecological cancers based on network pharmacological prediction and in vitro experimental validation. Integr Cancer Ther [Internet]. 2018;17(3):894–901. Available from:

Murthy Malladi S, Sastry Yarla N, Kumar Pandey D. Enzyme inhibition activity both in vitro and in silico screening of triphala plant extracts on phospholipase A2. Int J Pharm Investig [Internet]. 2021;11(2):158–64. Available from:

Chainani SH, Siddana S, Reddy C, Manjunathappa TH, Manjunath M, Rudraswamy S. Antiplaque and antigingivitis efficacy of triphala and chlorhexidine mouthrinse among schoolchildren - a cross-over, double-blind, randomised controlled trial. Oral Health Prev Dent [Internet]. 2014;12(3):209–217. Available from: PMID: 25197734

Srinagesh J, Pushpanjali K. Assessment of antibacterial efficacy of triphala against mutans streptococci: a randomised control trial. Oral Health Prev Dent. 2011;9(4):387-93. PMID: 22238738.

Pradeep AR, Suke DK, Martande SS, Singh SP, Nagpal K, Naik SB. Triphala, a New Herbal Mouthwash for the Treatment of Gingivitis: A Randomized Controlled Clinical Trial. J Periodontol. 2016 Nov;87(11):1352-1359. doi: 10.1902/jop.2016.130406. Epub 2016 Jul 21. PMID: 27442086.

Victor Arokia Doss, Prasad Maddisetty, Mohanasundaram.S. Biological active compounds with various medicinal values of Strychnos Nux-vomica – A Pharmacological summary. J Global Trends Pharm Sci 2016; 7(1): 3044-3047.

Ning W, Li S, Tsering J, Ma Y, Li H, Ma Y, Ogbuehi AC, Pan H, Li H, Hu S, Liu X, Deng Y, Zhang J, Hu X. Protective Effect of Triphala against Oxidative Stress-Induced Neurotoxicity. Biomed Res Int. 2021 Apr 7;2021:6674988. doi: 10.1155/2021/6674988. PMID: 33898626; PMCID: PMC8052154.

Sahragard A, Alavi Z, Abolhassanzadeh Z, Moein M, Mohammadi-Bardbori A, Omidi M, Zarshenas MM. Assessment of the Cytotoxic Activity of Triphala: A Semisolid Traditional Formulation on HepG2 Cancer Cell Line. Biomed Res Int. 2021 Aug 11;2021:6689568. doi: 10.1155/2021/6689568. PMID: 34471640; PMCID: PMC8405286.



How to Cite

K M, D., S., L. D., MOHANAN, A., & N. V., R. (2022). PHARMACOLOGY OF TRIPHALA WITH SPECIAL FOCUS ON THEIR CHEMICAL CONSTITUENTS. International Journal of Applied Pharmaceutics, 14. Retrieved from



Original Article(s)