ISONIAZID-BASED SCHIFF'S BASES IN BONE CANCER STUDIES USING MG-63 CELL LINES

Authors

  • MUNI HEMALATHA GILAKARA Vel Tech Rangarajan Dr. Sagunthala R&D Institute of Science and Technology, Chennai-600062, India. Sree Vidyanikethan Degree College, Affiliated to Sri Venkateswara University, Tirupathi, India.
  • KANDASAMY THIRUNAVUKKARASU 1. Vel Tech Rangarajan Dr. Sagunthala R&D Institute of Science and Technology, Chennai-600062, India
  • C. RAMESH KUMAR Department of Chemistry, Madras Christian College (Autonomous), University of Madras, Chennai 600059, India.

Keywords:

Isoniazid, inhibitary zone, MG63 cell lines, carcinoma, osteosarcoma cells, cytotoxicity, apoptosis assay, dual staining

Abstract

Introduction

 

We synthesized isoniazid based cation receptor Schiff bases (E)-N’-((1H-Pyrrol-2-yl) methylene) isonicotinohydrazide (S1) and (E)-N'-(thiophen-2-yl-methylene) isonicotinohydrazide (S2) supported heterocyclic derivatives by mechanical grinding followed by condensation reaction between their corresponding heterocyclic aldehydes and their H1 NMR, 13C NMR, & FT-IR results confirmed the expected structure.

 

Objective

Anticancer activities of Schiff bases S1 and S2 against MG-63 human osteosarcoma cells were performed and described using an in-vitro evaluation employing cytotoxicity and apoptosis assay.

 

Methodology

MG-63 cells were used in an MTT assay to examine the effect of the compound on cell viability (S1 and S2). Cell morphologies and IC50 values were obtained. The acridine orange (AO) /ethidium bromide (EB) dual staining technique was used to determine apoptosis process.

 

Results & Conclusion

Our findings showed that synthesised S1 and S2 reduced MG-63 cell proliferation and induced apoptosis in a dose-dependent manner, implying that they could be used to treat bone cancer.

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References

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Published

21-06-2022

How to Cite

GILAKARA, M. H., THIRUNAVUKKARASU, K., & KUMAR, C. R. (2022). ISONIAZID-BASED SCHIFF’S BASES IN BONE CANCER STUDIES USING MG-63 CELL LINES. International Journal of Applied Pharmaceutics, 14. Retrieved from https://innovareacademics.in/journals/index.php/ijap/article/view/45556

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