DEVELOPMENT AND EVALUATION OF NANOSPONGES BASED BUCCAL TABLETS FOR DELIVERY OF QUERCETIN USING BOX-BEHNKEN DESIGN
DOI:
https://doi.org/10.22159/ijap.2023v15i3.47120Keywords:
Quercetin, β-Cyclodextrin, Nanosponges, Experimental design, Buccal tablets, In-vitro drug releaseAbstract
Objective: The goal was to develop a controlled release formulation of quercetin utilizing the cyclodextrin based nanosponges as a nanocarrier.
Methods: Based on the preliminary trials a 3-factor, 3-level Box-Behnken design was employed. Five types of nanosponges from β-cyclodextrin (NS1-NS5) were purposely designed. Quercetin was loaded into nanosponges by freeze drying method. The prepared nanosponges were characterized and formulated into tablets and evaluated for weight variation, hardness, friability, disintegration studies, dissolution and stability studies.
Results: The particle sizes of quercetin-loaded nanosponges are in between 36.45 to 135.27 nm, encapsulation efficiency of 42.37 to 88.44 % and drug release% at 6h of 53.04 to 82.64 %. In-vitro release studies showed that more than 90 % of drug released from nanosponge formulations as compared to only around 45% from free drug suspension after 24 h. The FTIR, DSC and XRPD studies confirmed the interaction of Quercetin with nanosponges. TEM image revealed the spherical structure of drug loaded nanosponges. The drug loaded in the nanosponge structure can be retained and released slowly over time. The nanosponges were formulated in to tablets and evaluated for weight variation, hardness, friability and disintegration studies and obtained satisfactory results. In-vitro drug release from a tablet exhibited a maximum release of 99.75 percent with controlled release behaviour over 24 hours, and stability studies suggested no major significant changes within 6 months.
Conclusion: Cyclodextrin based nanosponges showed superior complexing ability with increased solubility of poorly soluble Quercetin tablets made for controlled drug delivery, which can reduce dosing frequency.
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