GREEN SYNTHESIZED ZNO NANOPARTICLES WITH CORDYCEPS MILITARIS FUNGUS EXTRACT FOR THE TREATMENT OF MEMORY IMPAIRMENT IN ALZHIEMER DISEASE

KHYATI SAINI; SATISH SHILPI; NAVEEN SINGHAL

doi:10.22159/ijap.2025v17i1.53491

Authors

KHYATI SAINI Department of Pharmaceutics, Faculty of Pharmacy, DIT University Dehradun, UK, India
SATISH SHILPI Department of Pharmaceutics, Faculty of Pharmacy, DIT University Dehradun, UK, India
NAVEEN SINGHAL Department of Chemistry, SOPS, DIT University Dehradun, UK, India

DOI:

https://doi.org/10.22159/ijap.2025v17i1.53491

Keywords:

ZnO nanoparticles, Optimization, Green synthesis, Anti acetyl-cholinesterase, Anti-Alzheimer disease

Abstract

Objective: Preparation of Zinc Oxide (ZnO) Nanoparticles (ZnNPs) by green synthesis process using Cordyceps militaris fungus extract and its assessment for the treatment of Alzheimer’s disease.

Methods: ZnO nanoparticles were synthesized using the green synthesis process with Cordyceps militaris fungus extract. The nanoparticles were characterized using various techniques, including SEM, TEM, XRD, EDAX, and optimization techniques to determine their shape, size, surface properties, and crystallinity.

Result: The results showed that the nanoparticles were spherical with a smooth surface, averaging 37.09 nm in size, and exhibited surface Plasmon Resonance at 300 nm. XRD analysis confirmed their crystalline structure. During synthesis, the suspension changed from dark yellow to colorless with cloudiness, indicating nanoparticle formation. The UV-Visible Spectroscopy revealed a SPR peak at 375 nm. Overall, the characterization confirmed the successful synthesis of zinc nanoparticles with desired properties. This study investigated the protective effects of green-synthesized Zinc nanoparticles on memory impairment in mice. Mice were divided into six groups and treated with various substances followed by sleep deprivation to induce memory impairment. Conclusion: Behavioral tests and biochemical analysis revealed the significantly improved cognitive function, reduced acetyl cholinesterase activity in a dose-dependent manner, comparable to Donepezil. Histopathological analysis confirmed the protective effects of Cordyceps militaris against memory impairment.

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References

Thema FT, Manikandan E, Dhlamini MS, Maaza MJ. Green synthesis of ZnO nanoparticles via Agathosma betulina natural extract. Mater Lett. 2015 Dec 15;161:124-7. doi: 10.1016/j.matlet.2015.08.052.

Mustafa EK, Liu G, Abd El Hamid HT, Kaloop MR. Simulation of land use dynamics and impact on land surface temperature using satellite data. GeoJournal. 2021;86(3):1089-107. doi: 10.1007/s10708-019-10115-0.

Sagar Raut DP, Thorat R. Green synthesis of zinc oxide (ZnO) nanoparticles using Ocimum tenuiflorum leaves. Int J Sci Res. 2015;4:1225-8.

Upadhyaya H, Shome S, Sarma R, Tewari S, Bhattacharya MK, Panda SK. Green synthesis characterization and antibacterial activity of ZnO nanoparticles. Am J Plant Sci. 2018;9(6):1279-91. doi: 10.4236/ajps.2018.96094.

Huda NU, Ghneim HK, Fozia F, Ahmed M, Mushtaq N, Sher N. Green synthesis of Kickxia elatine induced silver nanoparticles and their role as anti-acetylcholinesterase in the treatment of alzheimers disease. Green Process Synth. 2023;12(1):20230060. doi: 10.1515/gps-2023-0060.

Ceylan R, Demirbas A, Ocsoy I, Aktumsek A. Green synthesis of silver nanoparticles using aqueous extracts of three sideritis species from Turkey and evaluations bioactivity potentials. Sustain Chem Pharm. 2021;21:100426. doi: 10.1016/j.scp.2021.100426.

Suganthy N, Sri Ramkumar V, Pugazhendhi A, Benelli G, Archunan G. Biogenic synthesis of gold nanoparticles from Terminalia Arjuna bark extract: assessment of safety aspects and neuroprotective potential via antioxidant anticholinesterase and antiamyloidogenic effects. Environ Sci Pollut Res Int. 2018;25(11):10418-33. doi: 10.1007/s11356-017-9789-4, PMID 28762049.

Aminuzzaman M, Ying LP, Goh WS, Watanabe A. Green synthesis of zinc oxide nanoparticles using aqueous extract of Garcinia mangostana fruit pericarp and their photocatalytic activity. Bull Mater Sci. 2018;41:1-10.

Selim YA, Azb MA, Ragab I, HM Abd El Azim MM. Green synthesis of zinc oxide nanoparticles using aqueous extract of Deverra tortuosa and their cytotoxic activities. Sci Rep. 2020;10(1):3445. doi: 10.1038/s41598-020-60541-1, PMID 32103090.

Sharma J, Sweta S, Thakur C, Vats M, Sharma SK. Green synthesis of zinc oxide nanoparticles using neem extract. AIP Conf Proc. 2020;2220(1)20107. doi: 10.1063/5.0002093.

Ramola B, Joshi NC. Green synthesis characterisations and antimicrobial activities of CaO nanoparticles. Orient J Chem. 2019;35(3):1154-7. doi: 10.13005/ojc/350333.

Tripathi SK, Patel B, Shukla S, Pachouri C, Pathak S, Pandey A. Donepezil loaded PLGA nanoparticles from modified nano precipitation an advanced drug delivery system to treat alzheimer disease. J Phys Conf S. 2021;1849(1):12001. doi: 10.1088/1742-6596/1849/1/012001.

Zameer S, Ali J, Vohora D, Najmi AK, Akhtar M. Development optimisation and evaluation of chitosan nanoparticles of alendronate against alzheimers disease in intracerebroventricular streptozotocin model for brain delivery. J Drug Target. 2021;29(2):199-216. doi: 10.1080/1061186X.2020.1817041, PMID 32876502.

Karpagavinayagam P, Vedhi C. Green synthesis of iron oxide nanoparticles using Avicennia marina flower extract. Vacuum. 2019;160:286-92. doi: 10.1016/j.vacuum.2018.11.043.

Rautela A, Rani J. Green synthesis of silver nanoparticles from Tectona grandis seeds extract: characterization and mechanism of antimicrobial action on different microorganisms. J Anal Sci Technol. 2019;10(1):1-10.

Azarbani F, Shiravand S. Green synthesis of silver nanoparticles by Ferulago macrocarpa flowers extract and their antibacterial antifungal and toxic effects. Green Chem Lett Rev. 2020;13(1):41-9. doi: 10.1080/17518253.2020.1726504.

Behravan M, Hossein Panahi AH, Naghizadeh A, Ziaee M, Mahdavi R, Mirzapour A. Facile green synthesis of silver nanoparticles using berberis vulgaris leaf and root aqueous extract and its antibacterial activity. Int J Biol Macromol. 2019;124:148-54. doi: 10.1016/j.ijbiomac.2018.11.101, PMID 30447360.

Vijayakumar S, Mahadevan S, Arulmozhi P, Sriram S, Praseetha PK. Green synthesis of zinc oxide nanoparticles using Atalantia monophylla leaf extracts: characterization and antimicrobial analysis. Mater Sci Semicond Process. 2018;82:39-45. doi: 10.1016/j.mssp.2018.03.017.

Shoaib M, Naz A, Osra FA, Abro SH, Qazi SU, Siddiqui FA. Green synthesis and characterization of silver entecavir nanoparticles with stability determination. Arab J Chem. 2021;14(3):102974. doi: 10.1016/j.arabjc.2020.102974.

Abbas H, Sayed NS, Youssef NA, ME Gaafar P, Mousa MR, Fayez AM. Novel luteolin loaded chitosan decorated nanoparticles for brain targeting delivery in a sporadic alzheimers disease mouse model: focus on antioxidant anti-inflammatory and amyloidogenic pathways. Pharmaceutics. 2022;14(5):1003. doi: 10.3390/pharmaceutics14051003, PMID 35631589.

Krishna KV, Wadhwa G, Alexander A, Kanojia N, Saha RN, Kukreti R. Design and biological evaluation of lipoprotein based donepezil nanocarrier for enhanced brain uptake through oral delivery. ACS Chem Neurosci. 2019;10(9):4124-35. doi: 10.1021/acschemneuro.9b00343, PMID 31418556.

Higaki A, Mogi M, Iwanami J, Min LJ, Bai HY, Shan BS. Predicting outcome of morris water maze test in vascular dementia mouse model with deep learning. Plos One. 2018;13(2):e0191708. doi: 10.1371/journal.pone.0191708, PMID 29415035.

Mehla J, Deibel SH, Faraji J, Saito T, Saido TC, Mohajerani MH. Looking beyond the standard version of the morris water task in the assessment of mouse models of cognitive deficits. Hippocampus. 2019;29(1):3-14. doi: 10.1002/hipo.22999, PMID 30069957.

Rao SS, Lago L, Volitakis I, Shukla JJ, MC Coll G, Finkelstein DI. Deferiprone treatment in aged transgenic tau mice improves Y-maze performance and alters tau pathology. Neurotherapeutics. 2021;18(2):1081-94. doi: 10.1007/s13311-020-00972-w, PMID 33410108.

Sun Z, LI Q, BI K. Rapid HPLC-ESI-MS/MS analysis of neurotransmitters in the brain tissue of alzheimers disease rats before and after oral administration of Xanthoceras sorbifolia bunge. Molecules. 2018;23(12):3111. doi: 10.3390/molecules23123111, PMID 30486507.

Ghajarbeygi P, Hajhoseini A, Hosseini MS, Sharifan A. An in vitro and in vivo cholinesterase inhibitory activity of Pistacia khinjuk and allium sativum essential oils. J Pharmacopuncture. 2019;22(4):231-8. doi: 10.3831/KPI.2019.22.031, PMID 31970020.

Yadav E, Yadav P. Biofabricated zinc oxide nanoparticles impair cognitive function via modulating oxidative stress and acetylcholinesterase level in mice. Environ Toxicol. 2021;36(4):572-85. doi: 10.1002/tox.23062, PMID 33247493.

Hasnain MS, Javed MN, Alam MS, Rishishwar P, Rishishwar S, Ali S. Purple heart plant leaves extract mediated silver nanoparticle synthesis: optimization by box-behnken design. Mater Sci Eng C Mater Biol Appl. 2019;99:1105-14. doi: 10.1016/j.msec.2019.02.061, PMID 30889643.

Barzinjy AA, Azeez HH. Green synthesis and characterization of zinc oxide nanoparticles using Eucalyptus globulus Labill. leaf extract and zinc nitrate hexahydrate salt. SN Applied Sciences. 2020;2(5):991.

Kanniah P, Radhamani J, Chelliah P, Muthusamy N, Joshua Jebasingh Sathiya Balasingh E, Reeta Thangapandi J. Green synthesis of multifaceted silver nanoparticles using the flower extract of Aerva lanata and evaluation of its biological and environmental applications. Chemistry Select. 2020;5(7):2322-31. doi: 10.1002/slct.201903228.

El Belely EF, Farag MM, Said HA, Amin AS, Azab E, Gobouri AA. Green synthesis of zinc oxide nanoparticles (ZnO-NPs) using Arthrospira platensis (Class: Cyanophyceae) and evaluation of their biomedical activities. Nanomaterials (Basel). 2021;11(1):95. doi: 10.3390/nano11010095, PMID 33406606.

Singh P, Mijakovic I. Strong antimicrobial activity of silver nanoparticles obtained by the green synthesis in Viridibacillus sp. extracts. Front Microbiol. 2022;13:820048. doi: 10.3389/fmicb.2022.820048, PMID 35250934.

Heer AS. Spectral characterization and antifungal activity of zinc oxide (zno) nanoparticles synthesized using Cynodon dactylon leaf extract. World J Pharm Res. 2017;6(16):1588-96. doi: 10.20959/wjpr201716-10356.

Vijayan M, Bose C, Reddy PH. Protective effects of a small molecule inhibitor DDQ against amyloid beta in alzheimers disease. Mitochondrion. 2021;59:17-29. doi: 10.1016/j.mito.2021.04.005, PMID 33839321.

OU Z, Deng L, LU Z, WU F, Liu W, Huang D. Protective effects of akkermansia muciniphila on cognitive deficits and amyloid pathology in a mouse model of alzheimers disease. Nutr Diabetes. 2020;10(1):12. doi: 10.1038/s41387-020-0115-8, PMID 32321934.