SCREENING OF PHYTOCHEMICALS AND ANTIMICROBIAL POTENTIAL OF EXTRACTS OF VETIVER ZIZANOIDES AND PHRAGMITES KARKA AGAINST CLINICAL ISOLATES
Objective: To screen the phytochemicals present in extracts of Vetiver zizanoides and Phragmites karka. The antimicrobial potential was also determined against selected clinical isolates.
Methods: The antimicrobial potential of extracts from Vetiver zizanoides and Phragmites karka was evaluated by agar well diffusion method against selected clinical isolates. Preliminary phytochemical analysis and TLC-bioautographic method was also performed.
Results: Antimicrobial activity of Vetiver zizanoides and Phragmites karka extracts was assessed on nine bacterial and four fungal clinical isolates. The solvent extracts from both the grasses showed maximum activity against S. aureus 1, producing the maximum zone of inhibition 15Â±0.12 mm in Vetiveria zizanoides ethanol extract and 13Â±0.09 mm in diethyl ether extract of Phragmites karka. Preliminary phytochemical analysis demonstrated the presence of most of the phytochemicals including flavonoids, saponins, cardiac glycosides, tannins, alkaloids and steroids in both methanol and diethyl ether extracts or in any of them. Thin layer chromatography and bioautography assay in Vetiveria zizanoides methanol extracts demonstrated well defined growth inhibition zones against S. aureus 1 in correspondence with flavonoids observed at Rf value ranging from 0.63-0.75.
Conclusion: The present study opens future prospects of these plant constituents to be used as potential antimicrobial drug against infectious agents and can be used in treatment of various infectious diseases.
Keywords: Agar well diffusion, Clinical isolates, Phragmites karka, Phytochemical analysis, TLC bioautography, Vetiveria zizanoides.
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