PHYTOCHEMICAL ANALYSIS OF Aerva lanata, Adathoda vasica, Pisonia alba, Sesbania grandiflora AND Indigofera aspalathoides
The objective of the present study was to investigate the presence of various phytochemicals from ethanol, ethyl acetate, methanol and chloroform extracts of Aerva lanata (whole plant), leaves of Adathoda vasica, Pisonia alba, Sesbania grandiflora and Indigofera aspalathoides. The phytochemical analysis showed the presence of tannins, flavonoids, alkaloids, terpenoids, glycosides, saponins, resins, carbohydrates and proteins. The whole plant of A. lanata showed the presence of flavonoids, terpenoids, glycosides, carbohydrates and proteins. Flavonoids, alkaloids, terpenoids, glycosides, resins, carbohydrates and proteins are found in the leaves of Adathoda vasica. Flavonoids, glycosides and resins were found in the leaves of Pisonia alba. No phytochemical constituents were found in the chloroform extract of Pisonia alba leaves. Ethanol and methanol leaf extracts from Sesbania grandiflora and methanol leaf extract from Indigofera aspalathoides indicated that they contained most of the phytochemical compounds. The different extracts of plants have clearly indicated the presence of all the major phytochemicals, hence these plants can be used for the extraction of bioactive compounds.
2. Pierangeli G, Vital G, Rivera W: Antimicrobial activity and cytotoxicity of Chromolaena odorata (L. f) King and Robinson and Uncaria perrottetii (A. Rich) Merr Extracts. J. Medicinal Plants Res 2009; 3(7): 511-518.
3. Harmmer MM, Benice B, David L. Herbal alternative medicine use in an urban dental hygiene clinic. J Dent Hyg 2006; 1:19.
4. Haruna B, Adebola O. Preliminary Phytochemical Screening and Antimicrobial Evaluation of Three Medicinal Plants Used in Nigeria. African J Traditional Complement Altern Med 2011; 8(4): 387â€“390.
5. Sanaa OY, Shani EHAS, Braaha A, Asha ZEM. Antimicrobial activity of some medicinal plants against some Gram positive, Gram negative and fungi. African J Biotech 2007; 5(18): 1663-1668
6. Nostro A, GermanÃ² MP, D'angelo V, Marino A, Cannatelli MA. Extraction methods and bioautography for evaluation of medicinal plant antimicrobial activity. Lett Appl Microbiol 2000; 30: 379-384.
7. Krishnaiah D, Sarbatly R, Bono A. Phytochemical antioxidants for health and medicine: A move towards nature. Biotechnol Mol Biol Rev 2007; 1: 97-104.
8. Sony H, Sharma S, Patel SS, Mishra K. Preliminary phytochemical screening and HPLC analysis of flavonoid from methanolic extract of leaves of Annona squamosa. Int Res J Pharm 2011; 5: 242-246.
9. Karou D, Savadogo A, Canini A, Yameogo S, Montesano C, Simpore J, et al. Antibacterial activity of alkaloids from Sida acuta. African J Biotech 2006; 5: 195-200.
10. Yadav RNS, Munin A. Phytochemical analysis of some medicinal plants. J Phyto 2011; 3(12): 10-14.
11. Brain KR, Turner TD. Practical Evaluation of Phytopharmaceuticals. 1st ed. Bristol: Wright- Scientechnica; 1975.
12. Umesh BT, Hermalatha S, Anuj M. Pharmacognostic and phytochemical investigation on root of Cadaba farinosa Forsk. Inter J Pharma Bio Sci 2010; 1 (2):1-13.
13. Ciulei I. Methodology of analysis of vegetable drug. Romania. United Nations Industrial Development Organisation. 1994.
14. Evans WC, Trease GE. Pharmacognosy. 13th ed. London, 1989.
15. Harbrone JB. Phytochemical method: A guide to modern techniques of plant analysis. 3rd ed. New York: Chapman and Hall; 1998.
16. Ragavendran P, Sophia D, Chinthamony AR, Starlin T, Kanniappan GV. Phytochemical screening, antioxidant activity of Avera lanata (L) - An in vitro study. Asian J Pharm Clin Res 2012; 5(2): 77-79.
17. Koperuncholan M, Sathish P, Sathiyanarayanan G, Vivek G. Phytochemical screening and antimicrobial studies of some ethnomedicinal plants in South-Eastern slope of Western Ghats. Int J Medicobiol Res 2010; 1(1): 48-58.
18. Yamunadevi M, Wesely EG, Johnson M. Phytochemical studies on the terpenoids of medicinally important plant Aerva lanata L. Using HPTLC. Asian Pacific J Tropical Biomedi 2011; S220-S225.
19. Battu GR, Kumar BM. In-Vitro antioxidant activity of leaf extract of Aerva lanata Linn Int J Pharm Sci 2012; 2(4): 74-78.
20. Emimal EV. Pest infestation on the biochemical modulation of Adhatoda vasica. J Biopest 2010; 3(2): 413-419.
21. Bharathi B, Priya K, Arumugam P, Swamidos D. Phytochemical screening and antifungal activity of medicinal plants against opportunistic candida albicans of female HIV positive patients. Int J Ph Sci 2010; 2(3):804-809.
22. Subhashini S, Kantha D, Sathesh KA. Preclinical studies on the phytochemical, antimicrobial, and wound healing properties of Indigofera aspalathoides leaves. J Pharm Res 2011; 4(9): 3206-3211
23. Poongothai G, Shubashini KS. HPTLC method of quantification of bioactive marker constituent pinitol in the extract of Pisona grandis (R.BR). Int Res J Pharm 2012; 3(9): 207-212.
24. Nadia KJ, Shatha M, Mohammed A, Saadia S, Bassam A, Bayati MTN, et al. Comparative of phytochemical and antimicrobial of Sesbania grandiflora leaves. Medi J Babylon 2014; 11(3): 664-674.
25. Arun A, Karthikeyan P, Sagadevan P, Umamaheswari R, Rex PR. Phytochemical screening of Sesbania grandiflora (Linn). Int J Biosci Nanosci 2014; 1(2): 33-36.
26. Elangovan K, Sundaramoorthy T, Zahiruddin S, Kandasamy M. Phytochemical evaluation, in vitro antioxidant and antibacterial potentials of Indigofera aspalathoides . Int J Pharm Biol Sci 2014; 4(1):161-168.
27. Tamilselvi N, Krishnamoorthy P, Dhamotharan, R Arumugam P, Sagadevan E. Analysis of total phenols, total tannins and screening of phytocomponents in Indigofera aspalathoides (Shivanar Vembu) Vahl EX DC. J Chem Pharm Res 2012; 4(6):3259-3262.