ANTIBACTERIAL ACTIVITY OF SILVER NANOPARTICLES SYNTHESIZED FROM CLAUSENA ANISATA (WILLD.) HOOK F EX BENTH (RUTACEAE)
Objective: In the present study, the antibacterial activity of silver nanoparticles synthesized from Clausena anisata was analyzed by using agar well diffusion and disc diffusion method. Clausena anisata, a medicinal plant belonging to the family Rutaceae, is represented by 20 species available in India and used traditionally for the treatment of several ailments but there is a requirement to identify its phytoconstituents, its target, mode of action and treatment using plant products either alone or in combination with synthetic drugs.
Methods: Clausena anisata leaves and roots were procured from Manamettupatti, a village in Pudukottai District, Tamilnadu. The shade dried leaf and root was powdered and extracted using ethanol by maceration method. The extracts of leaf and root were further subjected to synthesis silver nanoparticles, characterized and the antibacterial activity was studied against Bacillus subtilis, Staphylococcus aurues, Escherichia coli, Proteus vulgaris, Pseudomonas aeruginosa and Provedencia rettgeri.
Results: The SNP leaf and root extracts showed antibacterial activity in the concentration ranging from 50 Âµg â€“ 150 Âµg/ml. All the extracts showed antibacterial activity against all the tested microorganisms. In which, the SNP leaf extract showed maximum zone of inhibition against P. aeruginosa (12Â±0.5) (P<0.0001), followed by Bacillus subtilis (P<0.0001), Staphylococcus aurues (P<0.0001), Proteus vulgaris (P<0.0001) and P. rettgeri (P<0.001) with each 10 mm in diameter and E. coli with 8Â±0.6 mm in diameter, where standard showed 15Â±0.57 mm in diameter against E.coli. The maximum zone of inhibition for SNP root extract was observed against E. coli (P<0.01) and P. retttgeri (P<0.01) with 15 mm in diameter each, followed by P. aeruginosa (P<0.0001), B. subtilis (P<0.0001), S. aureus (P<0.0001) Â and P. vulgaris (P<0.0001) with 14Â±2.5 mm, 12Â±1.52, 12Â±2.1 and 9Â±0.57 mm zone of inhibition respectively and standard showed 20Â±1.52 mm zone of inhibition against P. rettgeri.
Conclusion: The phytoconstituents of C. anisata SNP root extract may be acting synergistically or independently in exerting an overall antibacterial action in this study, and that should be chemically analyzed and their chemical structure should be understood in order to develop an effective antibacterial therapeutic agent in future.
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