• Sangeeta Huidrom Central Molecular Research Laboratory, Department of Biochemistry, Shri Guru Ram Rai Institute of Medical and Health Sciences, Dehradun 248001, India
  • R. K. Singh Central Molecular Research Laboratory, Department of Biochemistry, Shri Guru Ram Rai Institute of Medical and Health Sciences, Dehradun 248001, India
  • Varsha Chaudhary Central Molecular Research Laboratory, Department of Biochemistry, Shri Guru Ram Rai Institute of Medical and Health Sciences, Dehradun 248001, India


Probiotics, Prebiotics, Gut microbiome, Synbiotics, Immunomodulation


The alteration in the homeostasis of gut microbiota and harmful bacteria is associated with various kinds of diseases. Many gastrointestinal disorders such as inflammatory bowel diseases, irritable bowel syndrome, colorectal cancer and systemic diseases such as diabetes, obesity and atherosclerosis are due to dysbiosis in human gut. Many recent clinical studies revealed that probiotic is effective in treatment of various ailments by defending against colonization by opportunistic pathogens, production of antimicrobial substances and immunomodulation. Studies have shown that prebiotics and probiotics or the combinations of two i.e. synbiotics can restore the aberrant gut and can improve the health of gut. There is rapid growth and demand of dairy-based probiotics food in the market due to its awareness among the consumers. Bacteriotherapy has good hold future as novel therapy as consumers are looking for safe, cost effective and no adverse side effects therapeutic approach.


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Soccol CR, Vandenberghe LPS, Spier MR, Medeiros ABP, Yamaguishi CT, Lindner JDD. The potential of probiotics: a review. Food Technol Biotechnol 2010;48:413-34.

Metchnikoff E. Lactic acid as inhibiting intestinal putrefaction. The prolongation of life: Optimistic studies W Heinemann, London; 1907. p. 161-83.

Tissier H. The treatment of intestinal infections by the method of transformation of the intestinal bacterial flora. C R Soc Biol 1906;60:359–61.

Kunz C, Kuntz S, Rudloff S. Intestinal flora. Adv Exp Med Biol 2009;639:67–79.

Morrelli L. In vitro assessment of probiotic bacteria: from survival to functionality. Int Dairy J 2007;17:1278-83.

Neish AS. Microbes in gastrointestinal health and disease. Gastroenterology 2009;136:65–80.

Chiller K, Selkin BA, Murakawa GJ. Skin microflora and bacterial infections of the skin. J Invest Dermatol Symp Proc 2001;6:170–4.

Hull MW, Chow AW. Indigenous microflora and innate immunity of the head and neck. Infectious Disease Clin North Am 2007;21:265–82.

Verstraelen H. Cutting edge: the vaginal microflora and bacterial vaginosis. Verh K Acad Geneeskd Belg 2008;70:147–74.

Ley RE, Peterson DA, Gordon JI. Ecological and evolutionary forces shaping microbial diversity in the human intestine. Cell 2006;124:837–48.

Stecher B, Hardt WD. The role of microbiota in infectious disease. Trends Microbiol 2008;16:107-14.

McFarland LV. A review of the evidence of health claims for biotherapeutic agents. Microb Ecol Health Dis 2000;12:65-76.

Agheyisi R. The probiotics market: ingredients, supplements, foods, Report code: FOD035B, BCC Research, Wellesley, M A, USA; 2008.

Schrezenenmeir J, deVrese M. Probiotics, prebiotics, and synbiotics are approaching a definition. Am J Clin Nutr 2001;73:361S–4S.

Parker RB. Probiotics: the other half of the antibiotic story. Animal Nutrition Health 1974;29:4–8.

Çakır I. Determination of some probiotic properties on Lactobacilli and Bifidobacteria. Ankara University Thesis 2003;4:53–79.

Sanders ME. Probiotics: considerations for human health. Nutr Rev 2003;61:91-9.

FAO/WHO. Report of a joint FAO/WHO expert consultation on guidelines for the evaluation of probiotics in food. World Health Organization and Food and Agriculture Organization of the United Nations, London Ontario, Canada; 2002.

Elo S, Saxelin M, Salminen S. Attachment of Lactobacillus casei strain GG to human colon carcinoma cell line Caco-2: Comparison with other dairy strains. Lett Appl Microbiol 1991;13:154–6.

Vollaard EJ, Clasener HAL. Colonization resistance. Antimicrob Agents Chemother 1994;38:409–14.

Fuller R. Probiotics in man and animals. J Appl Bacteriol 1989;66:365–78.

Flynn S, van Sinderen D, Thornton GM, Holo H, Nes IF, Collins JK. Characterization of the genetic locus responsible for the production of ABP-118, a novel bacteriocin produced by the probiotic bacterium Lactobacillus salivarius subsp. salivarius UCC118. Microbiology 2002;148:973–84.

Zhang Z, Hinrichs DJ, Lu H, Chen H, Zhong W, Kolls JK. After interleukin-12p40, are interleukin-23 and interleukin-17 the next therapeutic targets for inflammatory bowel disease. Int Immunopharmacol 2007;7:409–16.

Mitsuoka T. Recent trends in research on intestinal flora. Bifidobact Microflora 1982;1:3-24.

Salminen S, Bouley MC, Boutron-Rualt MC, Cummings J, Franck A, Gibson G, et al. Functional food science and gastrointestinal physiology and function. Br J Nutr Suppl 1998;1:S147-S171.

Gibson GR, Roberfroid MB. Dietary modulation of the human colonic microbiota: introducing the concept of prebiotics. J Nutr 1995;125:1401-12.

Fooks LJ, Fuller R, Gibson GR. Prebiotics, probiotics and human gut microbiology. J Nutr 1999;9:53-61.

Scheinbach S. Probiotics: functionality and commercial status. Biotechnol Adv 1998;16: 581-608.

De Roos N, Katan M. Effects of probiotic bacteria on diarrhoea, lipid metabolism, and carcinogenesis: a review of papers published between 1988 and 1998. Am J Clin Nutr 2000;71:405-11.

Coté GA, Buchman AL. Review antibiotic-associated diarrhoea. Expert Opin Drug Saf 2006;5:361-72.

Siitonen S, Vapaatalo H, Salminen S, Gordin A, Saxelin M, Wikberg R, et al. Effect of lactobacillus GG yoghurt in prevention of antibiotic associated diarrhoea. Ann Med 1990;22:57-60.

Arvola T, Laiho K, Torkkeli S, Mykkanen H, Salminen S, Maunula I, et al. Prophylactic lactobacillus GG reduces antibiotic-associated diarrhoea in children with respiratory infections: a randomized study. Pediatrics 1999;104:64.

Vanderhoof J, Whitney D, Antonson D, Hanner T, Lupo J, Young R. Lactobacillus GG in the prevention of antibiotic-associated diarrhoea in children. J Pediatr 1999;135:564-8.

Saavedra J. Probiotics and infectious diarrhea. Am J Gastroenterol 2000;95:16-8.

Samaržija D, Tudor M, Prtilo T, Špehar I, Zamberlin S, Havranek J. Probiotic bacteria in prevention and treatment of diarrhea. Mljekarstvo 2009;59:28–32.

MacFarlane GT, Cummings JH. Probiotics, infection and immunity. Curr Opin Infect Dis 2002;15:501–6.

Majamaa H, Isolauri E. Probiotics: a novel approach in the management of food allergy. J Allergy Clin Immunol 1997;99:179–85.

Isolauri E, Arvola T, Sutas Y, Moilanen E, Salminen, S. Probiotics in the management of atopic eczema. Clin Exp Allergy 2000;30:1604–10.

Bliznakov G. Lipid-lowering drugs (statins), cholesterol, and coenzyme Q10. The Baycol case a modern Pandora’s box. Biomed Pharmacother 2002;56:56–9.

Mann GV. Studies of a surfactant and cholesteremia in the Maasai. Am J Clin Nutr 1974;27:464-9.

Sharper AG, Jones KW, Jones M, Kyobe J. Serum lipids in three nomadic tribes of northern Kenya. Am J Clin Nutr 1963;13:135-46.

Çakır I. Determination of some probiotic properties on Lactobacilli and Bifidobacteria. Ankara University Unpublished Thesis; 2003.

Dugas B, Mercenier A, Lenoir-Wijnkoop I, Arnaud C, Dugas N, Postaire E. Immunity, and probiotics. Trends Immunol Today 1999;20:387-90.

Adolfsson O, Meydani SN, Russell RM. Yogurt and gut function. Am J Clin Nutr 2004;80:245-56.

Seow S, Rahmat J, Mohamed A, Mahendran R, Lee Y, Bey B. Lactobacillus species is more cytotoxic to human bladder cancer cells than Mycobacterium bovis (BCG). J Urol 2002;168:2236-9.

Goldin BR, Gorbach SL, Saxelin M, Barakat S, Gualtieri L, Salminen S. Survival of Lactobacillus species (strain GG) in human gastrointestinal tract. Dig Dis Sci 1992;37:121–8.

Isolauri E, Juntunen M, Rautanen T, Sillanaukee P, Koivula T. A human Lactobacillus strain (Lactobacillus GG) promotes recovery from acute diarrhea in children. Paediatrics 1991;88:90-7.

Isolauri E, Kaila M, Mykkänen H, Ling WH, Salminen S. Oral bacteriotherapy for viral gastroenteritis. Dig Dis Sci 1994;39:2595-600.

Majamaa H, Isolauri E, Saxelin M, Vesikari T. Lactic acid bacteria in the treatment of acute rotavirus gastroenteritis. J Pediatr Gastroenterol Nutr 1995;20:333-8.

Black FT, Andersen PL, Orskov J, Orskov F, Gaarslev K, Laulund S. Prophylactic efficacy of lactobacilli on traveller’s diarrhea. In: Travel Medicine. ed. Steffen R. Conference on International Travel Medicine 1. Zurich, Switzerland, Berlin: Springer; 1989. p. 333–5.

Black F, Einarsson K, Lidbeck A, Orrhage K, Nord CE. Effect of lactic acid producing bacteria on the human intestinal microflora during ampicillin treatment. Scand J Infect Dis 1991;23:247–54.

Marteau P, Pochart P, Flourié B, Pellier P, Santos L, Desjeux JF, et al. Effect of chronic ingestion of a fermented dairy product containing Lactobacillus acidophilus and Bifidobacterium bifidum on metabolic activities of the colonic flora in humans. Am J Clin Nutr 1990;52:685–8.

Alm L, Ryd-Kjellen E, Setterberg G, Blomquist L. Effect of a new fermented milk product 'CULTURA' on constipation in geriatric patients. First Lactic Acid Bacteria Computer Conference Proceedings. Horizon Scientific Press, Norfolk, UK; 1993.

Link-Amster H, Rochat F, Saudan KY, Mignot O, Aeschlimann JM. Modulation of a specific humoral immune response and changes in intestinal flora mediated through fermented milk intake. FEMS Immunol Med Microbiol 1994;10:55–64.

Saavedra JM, Bauman NA, Oung I, Perman JA, Yolken RH. Feeding of Bifidobacterium bifidum and Streptococcus thermophiles to infants in the hospital for prevention of diarrhoea and shedding of rotavirus. Lancet 1994;344:1046–9.

Schiffrin EJ, Rochat F, Link-Amster H, Aeschlimann JM, Donnet-Hughes A. Immunomodulation of human blood cells following the ingestion of lactic acid bacteria. J Dairy Sci 1995;78:491–7.

Fukushima Y, Kawata Y, Hara H, Terada A, Mitsuoka T. Effect of a probiotic formula on intestinal immunoglobulin A production in healthy children. Food Microb 1998;42:39–44.

Aso Y, Akazan H. Prophylactic effect of a Lactobacillus casei preparation on the recurrence of superficial bladder cancer. Urol Int 1992;49:125–9.

Okawa T, Niibe H, Arai T, Sekiba K, Noda K, Takeuchi S, et al. Effect of LC9018 combined with radiation therapy on carcinoma of the uterine cervix. Cancer 1993;72:1949–54.

Tanaka R, Ohwaki M. A controlled study on the ingestion of Lactobacillus casei fermented milk on the intestinal microflora, its microbiology and immune system in healthy adults. Proceedings of XII Riken Symposium on Intestinal Flora, Tokyo, Japan; 1994. p. 85–104.

Aso Y, Akaza H, Kotake T, Tsukamoto T, Imai K, Naito S. Preventive effect of a Lactobacillus casei preparation on the recurrence of superficial bladder cancer in a double-blind trial. Eur Urol 1995;27:104–9.

Spanhaak S, Havenaar R, Schaafsma G. The effect of consumption of milk fermented by lactobacillus casei strain Shirota on the intestinal microflora and immune parameters in humans. Eur J Clin Nutr 1998;52:899–907.

Marteau P, Vaerman JP, Dehenin JP. Effect of intrajejunal perfusion and chronic ingestion of Lactobacillus acidophilus strain La1 on serum concentrations and jejunal secretions of immunoglobulins and serum proteins in healthy humans. Gastroenterol Clin Biol 1997;21:293–8.

Michetti P, Dorta G, Wiesel PH, Brassart D, Verdu E, Herranz M, et al. Effect of whey-based culture supernatant of Lactobacillus acidophilus (johnsonii) La1 on Helicobacter pylori infection in humans. Digestion 1999;60:203-9.

Donnet-Hughes A, Rochat F, Serrant P, Aeschlimann JM, Schiffrin EJ. Modulation of nonspecific mechanisms of defense by lactic acid bacteria: effective dose. J Dairy Sci 1999;82:863–9.

Johansson ML, Molin G, Jeppsson B. Administration of different Lactobacillus strains in fermented oatmeal soup: in vivo colonization of human intestinal mucosa and effect on the indigenous flora. Appl Environ Microbiol 1993;59:5–20.

Johansson ML, Nobaek S, Berggren A. Survival of Lactobacillus Plantarum DSM 9843 (299v), and effect on the short-chain fatty acid content of faeces after ingestion of a rose-hip drink with fermented oats. Int J Food Microbiol 1998;42:9–38.

Fujiwara S, Hashiba H, Hirota T, Forstner JF. Proteinaceousfactor(s) in culture supernatant fluids of bifidobacteria which prevents the binding of enterotoxigenic escherichia coli to ganglio tetraosyl ceramide. Appl Environ Microbiol 1997;63:506–12.

Chiang BL, Sheih YH, Wang LH, Liao CK, Gill HS. Enhancing immunity by dietary consumption of a probiotic lactic acid bacterium (Bifidobacterium lactis HN109): Optimization and definition of cellular immune responses. Eur J Clin Nutr 2000;54:849-55.

Gill HS, Rutherfurd KJ, Cross ML, Gopal PK. Enhancement of immunity in the elderly by dietary supplementation with the probiotic Bifidobacterium lactis HN019. Am J Clin Nutr 2001;74: 833-9.

Bouvier M, Meance S, Bouley C, Berta JL, Grimaud JC. Effects of consumption of milk fermented by the probiotic strain Bifidobacterium animalis DN-173 010 on colonic transit times in healthy humans. Biosci Microflora 2001;20:43-8.

Marteau P, Cuillerier E, Meance S, Gerhardt MF, Myara A, Bouvier M, et al. Bifidobacterium animalis strain DN-173 010 shortens the colonic transit time in healthy women: a double-blind, randomized, controlled study. Aliment Pharmacol Ther 2002;16:587-93.

Gibson GR, Probert HM, Van Loo JAE, Roberfroid MB. Dietary modulation of the human colonic microbiota: Updating the concept of prebiotics. Nutr Res Rev 2004;17:257–9.

Stowell J. Calorie control and weight management. In: Mitchell H. (ed) Sweeteners and sugar alternatives in food technology. Blackwell Publishing Ltd 2007;10. Doi:1002/9780470996003 ch4. [Article in Press]

Ziemer CJ, Gibson GR. An overview of probiotics, prebiotics, and synbiotics in the functional food concept: perspectives and future strategies. Int Dairy J 1998;8:473-9.

Crittenden RG, Karppinen S, Ojanen S. In vitro fermentation of cereal dietary fiber carbohydrates by probiotic and intestinal bacteria. J Sci Food Agric 2002;82:1–9.

Marcel Roberfroid. Prebiotics: the concept revisited. J Nutr 2007;137:830S–837S.

Bodera P. Influence of prebiotics on the human immune system (GALT). Recent Pat Inflammation Allergy Drug Discovery 2008;2:19–153.

Srinivasjois R, Rao S, Patole S. Prebiotic supplementation of formula in preterm neonates: a systematic review and meta-analysis of randomized controlled trials. Clin Nutr 2009;28:237–42.

Natasha Haskey, Wendy J. Dahl synbiotic therapy improves the quality of life and reduces symptoms in pediatric ulcerative colitis, ICAN: Infant, Child, and Adolescent Nutrition April; 2009;1:88-93.

Su P, Henriksson A, Mitchell H. Prebiotics enhance survival and prolong the retention period of specific probiotic inocula in an in vivo murine model. J Appl Microbiol 2007;103:2392–400.



How to Cite

Huidrom, S., R. K. Singh, and V. Chaudhary. “BACTERIOTHERAPY: A NOVEL THERAPEUTIC APPROACH”. International Journal of Current Pharmaceutical Research, vol. 8, no. 2, Apr. 2016, pp. 12-16,



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