IN VITRO ANTIBACTERIAL ACTIVITIES OF OPUNTIA FICUS INDICA STEM AND FRUIT EXTRACTS USING DISC DIFFUSION METHOD
Objective: Opuntia ficus indica is a medicinal plant belonging to family Cactaceae. It is a species of cactus that has long been a domesticated crop plant important in agricultural economies throughout arid and semiarid parts of the world. The fruit and stem are used to prepare worth added products, fruits jam, squash, drinks, preserve product of pickle, body lotion, shampoo and creams, etc.
Methods: For the preliminary study, various extracts of stem and fruit has been used to check the efficacy of antibacterial activity against Bacillus subtilis, Pseudomonas aeruginosa and Escherichia coli bacteria of gram-positive and gram-negative strain respectively using disc diffusion method.
Results: The stem and fruit extracts showed various levels of activity on different test organisms. The methanol fruit extracts showed high antibacterial activity against Escherichia Coli, Pseudomonas aeruginosa and Bacillus subtilis compared with other extracts. Aqueous extract of stem and fruit showed less antibacterial activity against the tested bacterial strains.
Conclusion: The present study suggests that the methanol extracts of the fruit of Opuntia ficus indica contain compounds that can form the basis for the development of a novel broad-spectrum antibacterial formulation.
2. Chougui N, Louaileche H, Mohedeb S, Mouloudj Y, Hammoui Y, Tamendjari A. Afr J Biotechnol 2013;12:299-307.
3. Dehbi F, Hasib A, Tilaoui M, El Batal H, Zaki N, Ouatmane A, et al. BSE risk and the use of meat and bone meal in the feed industry: perspectives in the context of relaxing control measures. Nat Sci Res 2013;21:3-12.
4. Dok-Go H, Lee KH, Kim HJ, Lee EH, Song JLY, Lee YH, et al. Neuroprotective effects of ant oxidative flavonoids, quercetin, -dihydro quercetin and quercetin 3-methyl ether, isolated from Opuntia ficus-indica var. saboten. Brain Res 2003;965:130â€“6.
5. Emad MA, Amna SK, Nazlina I. Antibacterial activity of oleo gum resins of commiphora molmol and Boswellia papyrifera against methicillin-resistant Staphylococcus aureus (MRSA). Sci Res Essays 2009;4:351-6.
6. Galati EM, Mondello MR, Giuffrida D, Dugo G, Miceli N, Pergolizzi S, et al. Chemical characterization and boil effects of Sicilian Opuntia ficus indica (L.) Mill. fruit juice: antioxidant and antiulcerogenic activity. J Agric Food Chem 2003;51:4903â€“8.
7. Galati EM, Montello M, Lauriano ER, Traviano MF, Galluzzo M, Miceli N. Opuntia ficus-indica (L.) Miller fruit juice protects liver from carbon tetrachloride-induced injury. Phytother Res 2005;19:796-800.
8. Hassan F, El-Razek A, Hassan AA. Nutritional value and hypoglycemic effect of cactus prickly pear (Opuntia Ficus-Indica) fruit juice in alloxan-induced diabetic rats. Aust J Basic Appl Sci 2011;5:356-77.
9. Kumar GS, Jayaveera KN, Ashok Kumar CK, Umachigi PS, Swamy BMV, Kishore kumar DV. Antimicrobial effects of Indian medicinal plants against acne-inducing bacteria. Trop J Pharm Res 2007;6:717-23.
10. Kuti J. Antioxidant compounds from four Opuntia cactus pear fruit varieties. Food Chem 2004;85:527-33.
11. Pesavento G, Calonico C, Bilia AR, Barnabei M, Calesini F, Addona R. Antibacterial activity of Oregano, Rosmarinus and thymus essential oils against Staphylococcus aureus and Listeria monocytogenes in beef meatballs. Food Control 2015;54:188-99.
12. Sanchez E, GarcÃa S, Heredia N. Extracts of edible and medicinal plants damage membranes of Vibrio cholerae. Appl Environ Microbiol 2010;76:6888-94.
13. Sathyabama S, Jayasurya Kinsley S, Sankaranarayanan S, Bama P. Antibacterial activity of different phytochemical extracts from the leaves of T. procumbens identification and mode of action of terpenoid compound as antibacterial. Int J Pharm Pharm Sci 2011;4:557-64.