• Madhaiyan Prabu Department of Biology, Gandhigram Rural Institute-Deemed University, Gandhigram 624302 Dindigul (TN), India
  • Renganathan Kumuthakalavalli Department of Biology, Gandhigram Rural Institute-Deemed University, Gandhigram 624302 Dindigul (TN), India


Objective: To evaluate the antioxidant activity of tropical edible mushrooms namely Pleurotus florida and Calocybe indica.

Methods: Antioxidant potential was evaluated by using various antioxidant assays such as DPPH free radical scavenging, hydroxyl radical scavenging, nitric oxide radical scavenging, and superoxide radical scavenging activities as well as lipid peroxidation inhibiting assay, reducing power assay, ferric reducing antioxidant power (FRAP), metal chelating activity, phospho-molybdenum reduction assay and anti-haemolytic activity.

Results: The results obtained from this antioxidant study strongly suggest that Pleurotus florida and Calocybe indica have significant antioxidant activity.

Conclusion: Edible mushrooms Pleurotus florida and Calocybe indica are having significant antioxidant activity, could serve as easily accessible natural food rich in antioxidant which may enhance the immune system against oxidative damage and may be utilized as the potential sources of therapeutic agents.

Keywords: Antioxidant, Pleurotus florida, Calocybe indica, Tropical edible mushrooms, Therapeutic agents


1. Wasser SP. Medicinal mushrooms as a source of antitumour and immunomodulating polysaccharides. Appl Microbiol Biotechnol 2002;60:258–74.
2. Royse DJ. Forward to the Fifth International Conference on mushroom biology and mushroom products. Acta Edulis Fungi 2005;12:1–2.
3. Prabu M, Kumuthakalavalli R. Nutritional and phytochemical studies on Pleurotus florida (Mont.) Singer and Calocybe indica P and C. World J Pharm Res 2014;3:4907–13.
4. Wong SP, Naidu M, David P, Bakar R, Vikineswary S. Neurogenerative potential of Lion's mane mushroom, Hericium erinaceus (Bull.: Fr.) Pers., (Higher Basidiomycetes), in the treatment of peripheral nerve injury. Int J Med Mushrooms 2012;14:427–46.
5. Suffness M, Douros J. Drugs of plant origin. In: DeVita VT, Busch H. editors. Methods in Cancer Research, Academic Press: New York; 1979;26:73–126.
6. Blois MS. Antioxidant determinations by the use of a stable free radical. Nature 1958;26:1199–200.
7. Klein BP, Perry AK. Ascorbic acid and Vitamin A activity in selected vegetables from different geographical areas of the United States. J Food Sci 1982;47:941–5.
8. Sreejayan N, Rao MNA. Nitric oxide scavenging activity Curcuminoids. J Pharm Pharmacol 1997;47:105–7.
9. McCord JM, Fridovich I. Superoxide dismutase: an enzymatic function for erythrocuprein. J Biol Chem 1969;244:6049.
10. Ohkawa H, Ohishi N, Yogi K. Assay of lipid peroxides in animal tissue by a thiobarbituric reaction. Ann Biochem 1979;95:351–8.
11. Oyaizu M. Studies on products of browning reaction prepared from glucose amine. Jpn J Nutr 1986;44:307–15.
12. Benzie IF, Strain JJ. The ferric reducing ability of plasma as a measure of "antioxidant power"-The FRAP assay. Ann Biochem 1996;239:70–6.
13. Dinis TCP, Madeira VMC, Almeida LM. The action of phenolic derivatives (acetaminophen, salicylate, and 5-aminosalicylate) as inhibitors of membrane lipid peroxidation and as peroxyl radical scavengers. Arch Biochem Biophys 1994;315:161–9.
14. Prieto P, Pineda M, Aguilar M. Spectrophotometric quantitative of antioxidant capacity through the formation of a phospho- molybdenum complex: specific application to the determination of vitamin E. Ann Biochem 1999;269:337–41.
15. Naim M, Gestetner BA, Birk Y. Antioxidative and antihemolytic activities of soybean isoflavones. J Agric Food Chem 1976;24:1174–7.
16. Kasuga A, Aoyaga Y, Sugahara T. Antioxidant activity of fungus Suillus bovines (L: Fr.) O. Kuntze. J Food Sci 1995;60:1113–5.
17. Elmastasa M, Isildaka O, Turkekulb I, Temura N. Determination of antioxidant activity and antioxidant compound in wild edible mushrooms. J Food Compost Anal 2007;20:337–45.
18. Gezer K, Duru ME, Kivrak I, Turkoglu A, Mercan N, Turkoglu H, et al. Free radical scavenging capacity and antimicrobial activity of wild edible mushroom from Turkey. Afr J Biotechnol 2006;5:1924–8.
19. Banerjee S, Sanjay KR, Chethan S, Malleshi NG. Finger millet (Eleusine coracana) polyphenols: Investigation of their antioxidant capacity and antimicrobial activity. Afr J Food Sci 2012;6:362−74.
20. Gutteridge MC. Reactivity of hydroxyl and hydroxyl-like radicals discriminated by the release of thiobarbituric acid reactive material from deoxy sugars, nucleosides, and benzoate. J Biochem 1984;224:761–7.
21. Spencer D, James EK, Ellis GJ, Shaw JE, Sprent JI. Interactions between rhizobia and potato tissue. J Exp Bot 1994;45:1475–82.
22. Halliwell B, Gutteridge JMC. Free radicals in biology and medicine. 3rd Edn. Oxford: Clarendon Press; 1999. p. 936.
23. Manian R, Anusuya N, Siddhuraju P, Manian S. The antioxidant activity and free radical scavenging potential of two different solvent extracts of Camellia sinensis (L.) O. Kuntz, Ficus bengalensis L. and Ficus racemosa L. Food Chem 2008;107:1000–7.
24. Menaga D, Rajakumar S, Ayyasamy PM. The free radical scavenging activity of methanolic extract of Pleurotus florida mushroom. Int J Pharm Pharm Sci 2013;5:601–6.
25. Baskar R, Lavanya R, Mayilvizhi S, Rajasekaran P. Free radical scavenging activity of antitumour polysaccharide fractions isolated from Ganoderma lucidum (Fr.) P. Karst. Nat Prod Radiance 2008;7:320–5.
26. Ilaentic A, Moncada S, Rosa DM. Modulation of adjuvant arthritis by endogenous nitric oxide. Br J Pharmacol 1993;110:701–6.
27. Sainani GS, Manika JS, Sainani RG. Oxidative stress: a key factor in the pathogenesis of chronic diseases. Nucl Med 1997;1:1.
28. Lata H, Ahuja GK. The role of free radicals in health and disease. Indian J Physiol Allied Sci 2000;57:124.
29. Khatri DK, Manohar K, Juvekar A. Preliminary phytochemical and antioxidant evaluation of a polyherbal formulation (Madhumi). Int J Phytopharmacol 2013;4:322–8.
30. Kokila N, Radha R, Jayshree N. In vitro antioxidant and antiarthritic activity of polyherbal formulation. J Pharmacogn Herb Formula 2013;13:10–5.
31. Karthika C, Chitra M, Radhika K. Protective activity of Shorea robusta leaf against oxidative stress in rats. Int J Pharm Sci Res 2013;4:4754–7.
32. Ruch RT, Cheng SJ, Klaunig JE. Spin trapping of superoxide and hydroxyl radicals. Method Enzymol 1984;105:198–209.
33. Siddhuraju P, Becker K. The antioxidant and free radical scavenging activities of processed cowpea (Vigna unguiculata L.) seed extracts. Food Chem 2007;101:10–9.
34. Braugghler JM, Duncan CA, Chase LR. The involvement of iron in lipid peroxidation, the importance of ferrous to ferric iron ion initiation. J Biol Biochem 1986;261:10282–9.
35. Khanam S, Shivprasad HN, Devi K. In vitro antioxidant screening models: a review. Indian J Pharm Educ Res 2004;38:180–3.
36. Selvi S, Umadevi P, Devipriya D, Chinnaswamy P. In vitro antioxidant and anti lipid peroxidative potential of Calocybe indica. J Nat Rem 2010;10:27–31.
37. Meir S, Kanner J, Akiri B, Hadas SP. Determination and involvement of aqueous reducing compounds in oxidative defense systems of various senescing leaves. J Agric Food Chem 1995;43:1813–9.
38. Oliveira MT, Rego AC, Macedo TRA, Oliveira R. Drugs of abuse induces apoptotic features in PC12 cells. Ann N Y Acad Sci 2003;1010:667–70.
39. Zou Y, Lu Y, Wei D. Antioxidant activity of a flavonoid-rich extract of Hypericum perforatum L. in vitro. J Agric Food Chem 2004;52:5032–9.
40. Chung KH, Hart CC, Al-Bassam S, Avery A, Taylor J, Patel PD, et al. Polycistronic RNA polymerase II expression vectors for RNA interference based on BIC/miR-155. Nucleic Acids Res 2006;34:1–14.
41. Nabavi SM, Ebrahimzadeh MA, Nabavi SF, Jafari M. Free radical scavenging activity and antioxidant capacity of Eryngium caucasicum Trautv and Froripia subpinnata. Pharmacol 2008;3:19–25.
42. Kekuda TRP, Vinayaka KS, Swathi D, Suchitha Y, Venugopal TM, Mallikarjun N. Mineral composition, total phenol content and antioxidant activity of a Macrolichen Everniastrum cirrhatum (Fr.) Hale (Parmeliaceae). E J Chem 2011;8:1886–94.
43. Yildirima NC, Turkoglub S, Yildirima S, Inceb OK. Antioxidant properties of wild edible mushroom Pleurotus eryngii collected from the tunnel province of Turkey. Digest J Nanomater Biostructures 2012;7:1647–54.
44. Ema A, Sathish Kumar M, Jeyanthi LR, Sindhu S, Anbarasi P, Sagadevan, et al. Evaluation of the antiproliferative effect of Grewia hirsuta on HepG2 cell lines. J Acad Ind Res 2013;2:1–5.
45. Goodman KT, Scott PM. Risk assessment of mycotoxin ochratoxin A. Biomed Environ Sci 1989;2:179–48.
46. Ebrahimzadeh MA, Pourmorad F, Bekhradnia AR. Iron chelating activity screening, phenol and flavonoid content of some medicinal plants from Iran. Afr J Biotechnol 2008;7:3188–92.
47. Devjani C, Verma RJ. Ameliorative effect of Emblica officinalis aqueous extract against ochratoxin induced lipid peroxidation in the kidney and liver of mice. Int J Occupational Med Environ Health 2010;23:1–11.
How to site this article
• Madhaiyan Prabu, Renganathan Kumuthakalavalli. Antioxidant activity of oyster mushroom (Pleurotus Florida [Mont.] Singer) and milky mushroom (Calocybe Indica P and C). Int J Curr Pharm Res 2016;8(3):??
487 Views | 1753 Downloads
How to Cite
Prabu, M., and R. Kumuthakalavalli. “ANTIOXIDANT ACTIVITY OF OYSTER MUSHROOM (PLEUROTUS FLORIDA [MONT.] SINGER) AND MILKY MUSHROOM (CALOCYBE INDICA P AND C)”. International Journal of Current Pharmaceutical Research, Vol. 8, no. 3, July 2016, pp. 48-51,
Original Article(s)