ANTIPROLIFERATIVE AND ANTIOXIDANT ACTIVITY OF LEAVES EXTRACTS OF MORINGA OLEIFERA


Burli Sanganna, Havagiray R. Chitme, Khanvilkar Vrunda, Mohsin J. Jamadar

Abstract


Objective: The aim of the study was to investigate the ethanolic and aqueous extract of leaves of Moringa oleifera for phytochemical constituents, antiproliferative and antioxidant activity.

Methods: The ethanolic extract of leaves of Moringa oleifera, belonging to the family Moringaceae was prepared by using soxhlet apparatus and aqueous extract was prepared by using maceration process. The extract was evaluated for its phytochemical constituents. The antiproliferative effects of both extracts were checked by using MTT ([3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide]) assay on HT-29 colon cell line and the antioxidant activity were checked by using DPPH (2,2-diphenyl-1-picrylhydrazyl) assay. In antiproliferative and antioxidant activity the 5-FU (5-fluro uracil) and Ascorbic acid used as a standard drug for present results conclusion respectively.

Results: The results obtained in MTT assay shown that ethanolic extract of Moringa oleifera had a more potent antiproliferative effect (growth inhibition of 62.25% at 100 μg/ml) on HT-29 colon cell line as compared to aqueous extract (% growth inhibition of 27.86 at 100 μg/ml) of Moringa oleifera. The ethanolic extract of Moringa oleifera shown more potent antioxidant activity (% inhibition of ethanolic 75.57 at 100 μg/ml) than aqueous extract (38.16 at 100 μg/ml) of Moringa oleifera. The activity shown by the extract is concentration dependent.

Conclusion: In the present study we have investigated that the effect of ethanolic and aqueous leaves extracts of Moringa oleifera possess antiproliferative and antioxidant properties.

Keywords


Antiproliferative, Antioxidant, MTT assay, DPPH assay, HT-29 colon cell line

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About this article

Title

ANTIPROLIFERATIVE AND ANTIOXIDANT ACTIVITY OF LEAVES EXTRACTS OF MORINGA OLEIFERA

Keywords

Antiproliferative, Antioxidant, MTT assay, DPPH assay, HT-29 colon cell line

DOI

10.22159/ijcpr.2016v8i4.15278

Date

18-10-2016

Additional Links

Manuscript Submission

Journal

International Journal of Current Pharmaceutical Research
Vol 8, Issue 4, 2016 Page: 54-56

Online ISSN

0975-7066

Statistics

44 Views | 145 Downloads

Authors & Affiliations

Burli Sanganna
Department of Pharmacology, Tatyasaheb Kore College of Pharmacy, Warananagar 416113

Havagiray R. Chitme
Departments of Pharmacy, Oman Medical College Oman Medical College, Bausher Campus, Muscat-Oman

Khanvilkar Vrunda
Department of Pharmacology, Tatyasaheb Kore College of Pharmacy, Warananagar 416113

Mohsin J. Jamadar
Appasaheb Birnale College of Pharmacy, Sangli Depatment of Quality Assurance


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