EXPERIMENTAL EVALUATION OF ANTIDEPRESSANT AND ANTIANXIETY ACTIVITIES OF AQUEOUS LEAF EXTRACTS OF SENNA ALATA (L.) ROXB. USING IN VITRO ANIMAL MODELS


Archana Pamulaparthi, Vamshi Ramana Prathap, Mahitha Banala, Rama Swamy Nanna

Abstract


Objective: In the present investigation antidepressant and antianxiety activities of aqueous leaf extracts of Senna alata (200 mg/Kg) were carried out to establish the species as a potent natural antidepressant and anxiolytic drug.

Methods: Antidepressant activity was carried out using forced swim test and tail suspension test. In both these tests, the animals were subjected to external stress that results in alteration of the behavior of animal due to fear.

The antianxiety activity of aqueous leaf extracts of S. alata has been studied by the elevated plus-maze test in rats. The mean number of entries and the time spent in the open arm after 45 min of the administration of test drug was noted to determine the antianxiety effect of the test drug.

Results: For antidepressant activity administration of test drug (200 mg/Kg) showed a significant decrease in the time spent by the animal in state of depression in both the assays which clearly indicates that the aqueous leaf extracts of S. alata exhibited a strong antidepressant activity similar to that of the control drug (Imipramine).

In the antianxiety activity, administration of aqueous leaf extract (200 mg/Kg) of S. alata significantly increased the mean number of entries (2.25±0.98) in the open arm and the time spent in open arm (2.23±0.04) compared to the control group. The activity of the extract was slightly greater than standard drug Diazepam.

Conclusion: From the above-presented results, it can be concluded that administration of Senna alata aqueous leaf extracts (200 mg/Kg) showed a considerable decrease in both antidepressant and antianxiety activities in all the test animals and can be used in the replacement of commercially available synthetic drugs in the near future.

Keywords


Antianxiety activity, Antidepressant activity, Aqueous leaf extracts, Senna alata

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References


WHO. The World Health Report. Mental health: New understanding new hope. WHO, Geneva; 2001.

Reynolds EH. Brain and mind: a challenge for WHO. Lancet 2003;361:1924–5.

Mamedov N. Adaptogenic, geriatric, stimulant and antidepressant plants of russian far East. J Cell Mol Biol 2005;4:71-5.

Umadevi P, Murugan S, Suganthi SJ, Subakanmani S. Evaluation of antidepressant-like activity of Cucurbita pepo seed extracts in rats. Int J Curr Pharm Res 2011;3:108-13.

Batool F, Kamal A, Sattar M, Shah AH, Ahmed SD, Saify ZS, et al. Evaluation of antidepressant-like effects of aqueous extract of sea Buckthorn (Hippophae rhamnoides Linn Ssp. Turkestanica) fruits in experimental models of depression. Pakistan J Bot 2011;43:1595-9.

Sharma MC, Sharma S, Kohli DV. Some plant and extracts used in the pharmacologically activity of anxiolytics, antidepressant, analgesic, and anti-inflammatory activity. Digest J Nonmaterial Biostructures 2010;5:223-7.

Moinuddin G, Devi K, Satish H, Khajuria DK. Comparative pharmacological evaluation of Ocimum sanctum and imipramine for antidepressant activity. Lat Am J Pharm 2010;30:435-9.

Saba Shafeen, Srinath reddy T, Arafath S, Nagarjuna S, Padmanabha reddy Y. Evaluation of the antianxiety and antidepressant activity of Cassia occidentalis leaves. Asian J Pharm Clin Res 2012;5:47-50.

Kumar R, Murugananthan G, Nandakumar K, Talwar S. Isolation of anxiolytic principle from ethanolic root extract of Cardiospermum halicacabum. Phytomedicine 2011;18:219-23.

Zhang ZJ. Therapeutic effects of herbal extracts and constituents in animal models of psychiatric disorders. Life Sci 2004;75:1659-99.

Bhadoriya BU, Yadov A, Aggarwal N, Jaswal D, Yadov IK, Singh HP, et al. Hypnotic effect of essential oil and methanolic extract of fruits of Zanthoxylum budrunga W. Int J Pharm Tech Res 2009;4:1494-8.

Badgujar VB, Surana SJ. Anxiolytic effects of Dolichandrone falcata Seem., Bignoniaceae, stem-bark in elevated plus-maze and marble burying test on mice. Rev Bras Farmacogn 2010;20:773-80.

Bum EN, Ngoupaye GT, Talla E, Dimo TG, Nkantchoua CN, Pelanken MM, et al. The anticonvulsant and sedative properties of stems of Cissus quadrangularis in mice. Afr J Pharm Pharmacol 2008;2:42-7.

Sandeep Goyal, Suresh Kumar. Anti-anxiety activity studies of various extracts of Pulsatilla nigricans stoerck. Int J Pharm Sci Drug Res 2010;2:291-3.

Akindele AJ, Adeyemi OO. Anxiolytic and sedative effects of Byrsocarpus coccineus schum and Thonn. (Connaraceae) extract. Int J Appl Res Nat Prod 2010;3:28-36.

Palanichamy S, Nagarajan S, Devasagayam M. Effect of Cassia alata leaf extract on hyperglycemic rats. J Ethnopharmacol 1988;22:81-90.

Crockett CO, Guede-Guina F, Pugh D, Vangah-Manda M, Robinson TJ, Olubadewo JO, et al. Cassia alata and the preclinical search for therapeutic agents for the treatment of opportunistic infections in AIDS patients. Cell Mol Biol 1992;38:799-803.

Damodaran S, Venkataraman S. A study on the therapeutic efficacy of Cassia alata Linn. Leaf extract against Pityriasis versicolor. J Ehtnopharmacol 1994;42:19-23.

Steru L, Chermat R, Thierry B, Simon P. The tail suspension test: a new method for screening antidepressants in mice. Psychopharmacology (Berl.) 1985;85:367–70.

Porsolt RD, Bertin A, Jalfre M. Behavioural despair in mice: a primary screening test for antidepressants. Arch Int Pharmacodyn Ther 1977;229:327-36.

Kulkarni SK, Reddy DS. Animal behavioral models for testing anti-anxiety agents. Methods Find Exp Clin Pharmacol 1996;18:219-30.

Borsini F, Meli A. Is the forced swimming test a suitable model for revealing antidepressant activity? Psychopharmacology 1988;94:147–60.

Vikas Gupta, Parveen Bansal, Pawan Kumar, Richa Shri. Anxiolytic and antidepressant activities of different extracts from Citrus paradisi var. Duncan. Asian J Pharm Clin Res 2010;3:98-100.

Vimal Kant Sharma, Nagendra Singh Chauhan, Santram Lodhi, AK Singhai. Antidepressant activity of Zizyphus xylopyrus. Int J Phytomed 2009;1:12-7.

Youdim KA, Qaiser MZ, Begley DJ, Rice-Evans CA, Abbott NJ. Corrigendum to flavonoid permeability across an in situ model of the blood–brain barrier. Free Radical Biol Med 2004;36:592-604.

Jung JW, Ahn WY, Oh HR, Lee BR, Lee KJ, Kim SY, et al. Anxiolytic effect of the aqueous extract of Uncaria rhynchophyll. J Ethnopharmacol 2006;108:193-7.

Graeff FG, Guimares FS, De Andrade TG, Deakin JF. The role of 5-HT in stress, anxiety and depression. Pharmacol Biochem Behav 1996;54:129–41.

Paladini AC, Marder M, Viola H, Wolfman C, Wasowaki C, Medina JH. Flavonoids and the central nervous system: from forgotten factors to potent anxiolytic compounds. J Pharm Pharmacol 1999;51:519-26.

Medina JH, Viola H, Wolfman C, Marder M, Wasowski C, Clavo D, et al. Neuroactive flavonoids: new ligands for the benzodiazepine receptors. Phytomedicine 1997;5:235-43.

Wolfman C, Viola H, Paladini A, Dajas F, Medina JH. Possible anxiolytic effects of chrysin, a central benzodiazepine receptor ligand isolated from Passiflora coerulea. Pharmacol Biochem Behav 1994;47:1-4.

Bhattacharya SK, Satyan KS. Experimental methods for evaluation of psychotropic agents in rodents: I-anti-anxiety agents. Indian J Exp Biol 1997;35:565-75.




About this article

Title

EXPERIMENTAL EVALUATION OF ANTIDEPRESSANT AND ANTIANXIETY ACTIVITIES OF AQUEOUS LEAF EXTRACTS OF SENNA ALATA (L.) ROXB. USING IN VITRO ANIMAL MODELS

Keywords

Antianxiety activity, Antidepressant activity, Aqueous leaf extracts, Senna alata

DOI

10.22159/ijcpr.2016v8i4.15280

Date

18-10-2016

Additional Links

Manuscript Submission

Journal

International Journal of Current Pharmaceutical Research
Vol 8, Issue 4, 2016 Page: 60-63

Online ISSN

0975-7066

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80 Views | 114 Downloads

Authors & Affiliations

Archana Pamulaparthi
Plant Biotechnology Research Group, Department of Biotechnology, Kakatiya University, Warangal 506009, TS, India
India

Vamshi Ramana Prathap
Jawaharlal Nehru Technological University, Hyderabad 500085, TS, India

Mahitha Banala
Plant Biotechnology Research Group, Department of Biotechnology, Kakatiya University, Warangal 506009, TS, India

Rama Swamy Nanna
Plant Biotechnology Research Group, Department of Biotechnology, Kakatiya University, Warangal 506009, TS, India
India


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