EVALUATION OF ANTIEPILEPTIC ACTIVITY OF ETHANOLIC EXTRACT OF AZIMA TETRACANTHA ROOT IN MICE


Madhavi Eerike, Venu Gopala Rao Konda, Ruckmani Arunachalam, Umar Dawood

Abstract


Objective: To evaluate the antiepileptic activity of ethanolic extract of Azima tetracantha root (EEATR) against Maximal electroshock (MES) and Pentylene tetrazole (PTZ) induced seizures in mice.

Methods: 48 adult male mice were used and 4 groups with six in each were allocated to each model. 4 Groups are divided into control, standard and two test groups. Control group received normal saline, standard group, Sodium valproate-200 mg/kg and the two test groups received ethanolic extract of roots of Azima tetracantha (EEATR) 250 and 500 mg/kg respectively. Antiepileptic activity was assessed based on hind limb tonic extension duration, onset of convulsions and mortality. The results were compared with control and standard.

Results: In MES model EEATR reduced the duration of hind limb extension (HLE) and seizure protection was 50% and 66.6% with 250 and 500 mg/kg respectively. In PTZ model both the doses of EEATR delayed the onset of clonic phase and prevented death in 50% of animals in group treated with 500 mg/kg EEATR, similar to sodium valproate. Results were analyzed by ANOVA with p<0.05 considered as significant.

Conclusion: EEATR has shown anticonvulsant activity in both MES and PTZ models. 500 mg/kg of EEATR has better protection than 250 mg/kg against seizure in MES model and equally efficacious as sodium valproate standard in PTZ model.


Keywords


Antiepileptic activity, Maximal Electroshock (MES), Pentylene tetrazole (PTZ), Azima tetracantha root, Sodium Valproate

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About this article

Title

EVALUATION OF ANTIEPILEPTIC ACTIVITY OF ETHANOLIC EXTRACT OF AZIMA TETRACANTHA ROOT IN MICE

Keywords

Antiepileptic activity, Maximal Electroshock (MES), Pentylene tetrazole (PTZ), Azima tetracantha root, Sodium Valproate

DOI

10.22159/ijcpr.2016v8i4.15385

Date

18-10-2016

Additional Links

Manuscript Submission

Journal

International Journal of Current Pharmaceutical Research
Vol 8, Issue 4, 2016 Page: 76-79

Online ISSN

0975-7066

Statistics

75 Views | 68 Downloads

Authors & Affiliations

Madhavi Eerike
Dept of Pharmacology, Chettinad Hospital and Research Institute Kelambakkam, Kanchipuram (dt), Tamil Nadu 603103, India
India

Venu Gopala Rao Konda
Dept of Pharmacology, Chettinad Hospital and Research Institute Kelambakkam, Kanchipuram (dt), Tamil Nadu 603103, India
India

Ruckmani Arunachalam
Dept of Pharmacology, Chettinad Hospital and Research Institute Kelambakkam, Kanchipuram (dt), Tamil Nadu 603103, India
India

Umar Dawood
Dept of Pharmacology, Ragas Dental College, East Coast Road, Uthandi, Chennai 600096, Tamilnadu, India
India


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