EFFECT OF RASA MEZHUGU ON FREUND'S ADJUVANT-INDUCED ARTHRITIS IN RATS
Keywords:Rasa Mezhugu, Palm Jaggary, Indomethacin and Rheumatoid Arthritis
Objective: Rheumatoid arthritis is a long-lastingÂ autoimmune disorderÂ that primarily affectsÂ joints. It typically results in warm, swollen, andÂ painfulÂ joints. Pain and stiffness often worsen the following rest. The goal of treatment is to reduce pain, decrease inflammation, and improve a person's overall functioning. In spite of tremendous development in the field of synthetic drugs during recent years, the side effects could not be avoided. Hence the current research focus is to develop less toxic drugs as early as feasible in the disease process.
Methods: A 90 d old Wistar male rat was used for the research and was divided into four groups treated with Saline, Palm Jaggary, Rasa Mezhugu and Indomethacin.
Results: Acute toxicity study, Sub Acute study, Anti-arthritic study and histological findings showed that the Rasa Mezhugu has an anti-arthritis effect.
Conclusion: Further the histopathology slide photos of bone, cerebrum, cardiac muscle, kidney, liver, pancreas, ovaries, lungs, testes, stomach and spleen proved the safety of Rasa Mezhugu in rats.
Henderson B, Pettipher ER, Higgs GA. Mediators of rheumatoid arthritis. Br Med Bull 1987;42:415-28.
Findlay DM, DR Haynes. Mechanisms of bone loss in rheumatoid arthritis. Mod Rheumatol 2005;15:232-40.
Atzeni F, P Sarzi-Puttini. Early rheumatoid arthritis. Reumatismo 2007;59:100-17.
Fairhurst AM, PK Wallace, AS Jawad, NJ Goulding. Rheumatoid peripheral blood phagocytes are primed for activation but have impaired Fc-mediated generation of reactive oxygen species. Arthritis Res Ther 2007;9:R29-R29.
Hazeena BV, Sadique J. Long-term effect of herbal drug Withania somnifera on adjuvant-induced arthritis in rats. Indian J Exp Biol 1988;26:877-82.
Hoffmann JC, C Herklotz, H Zeidler, B Bayer, H Rosenthal, J Westermann. Initiation and perpetuation of rat adjuvant arthritis is inhibited by the anti-CD2 monoclonal antibody (mAb) OX34. Ann Rheum Dis 1997;56:716-22.
Fereidoni M, An Ahmadiani, S Semnanian, M Javan. An accurate and simple method for measurement of paw edema. J Pharmacol Toxicol Methods 2000;43:11-4.
OECD Guideline for the testing of chemicals, Acute Oral Toxicity: Up-and-Down Procedure 425; 1998. p. 1-8.
OECD Guidelines for the testing of chemicals. Repeated Dose 28-Day oral toxicity study in rodents 407; 2008. p. 1-13.
Ghosh MN. Fundamentals of experimental pharmacology. 2nd Edn. Scientific book agency, Kolkatta; 1994. p. 153-8.
Hurley JV, DA Willoughby. Acute inflammation: a combined topographical and electron microscopic study of the mode of action of carrageenan. Pathology 1973;5:9-21.
The disease-modifying activity of SK and F 106615 in rat adjuvant-induced arthritis. Multiparameter analysis of disease magnetic resonance imaging and bone mineral density measurements. Arthritis Rheum 1996;39:504-14.
Chen XY, Li J, Cheng WM, Jiang H, Xie XF, Hu R. Effect of total flavonoids of Chrysanthemum Indicum on the apoptosis of synoviocytes in joint of adjuvant arthritis rats. Am J Chin Med 2008;36:695â€“704.
Abotsi WMK, E Woode, GK Ainooson, An Amo-Barimah, E Boakye-Gyasi. Antiarthritic and antioxidant effects of the leaf extract of ficus exasperata P. Beauv. (Moraceae). Pharmacogn Res 2010;2:89-97.
Agarwal SK, MB Brenner. The role of adhesion molecules in synovial inflammation. Curr Opin Rheumatol 2006;18:268-76.
Akaogi J, T Nozaki, M Satoh, H Yamada. The role of PGE2 and EP receptors in the pathogenesis of rheumatoid arthritis and as a novel therapeutic strategy. Endocr Metab Immune Disord Drug Targets 2006;6:383-94.