• Anindita Banerjee Department of Physiology, Serampore College, Hooghly, West Bengal, India
  • Bithin Maji Department of Physiology, Serampore College, Hooghly, West Bengal, India
  • Sandip Mukherjee Department of Physiology, Serampore College, Hooghly, West Bengal, India
  • Kausik Chaudhuri Plant Chemistry Department, Botanical Survey of India, Howrah, India
  • Tapan Seal Plant Chemistry Department, Botanical Survey of India, Howrah, India


Objective: The aim of the present study was to evaluate the alpha (α)-amylase and alpha (α)-glucosidase inhibitory activities and in vitro antioxidant activities of the 80 % aqueous ethanol extracts of Tinosporasinensis Lour (Merr.).

Methods: The 80% aq. ethanol extract of the plant was prepared. The plant extract was examined for its antioxidant activity by using free radical 1,1-diphenyl-2-picryl hydrazyl (DPPH) scavenging method, ABTS radical scavenging ability, reducing power capacity, estimation of total phenolic content, flavonoid content and flavonol content. Different concentrations (2, 4, 8,10and 15 μg/ml) of the extract was subjected to α-amylase inhibitory and α-glucosidase inhibitory activities and IC50were calculated.

Results: The study revealed that the different concentrations of the plant extract possessed a very good amount of total phenolics, flavonoid and flavonol and exhibited potent radical scavenging activity using DPPH and ABTS as a substrate. The ethanol extracts exhibited significant α-amylase and α-glucosidase inhibitory activities with an IC50 value1.093µg and 1.04µg dry extract respectively and well compared with standard acarbose drug.

Conclusion: Thus, it could be concluded that due to the presence of antioxidant components the plant extracts could be used for the treatment of hyperglycemia, diabetes and the related condition of oxidative stress. This knowledge will be useful in finding more potent components from the natural resources for the clinical development of antidiabetic therapeutics.

Keywords: Tinosporasinensis, 80% aq. ethanol extract, Antioxidant activities, Nil, Nil


1. Keerthana G, Kalaivani MK, Sumathy A. In vitro alpha-amylase inhibitory and antioxidant activities of an ethanolic leaf extract of Croton bonplandianum. Asian J Pharm Clin Res 2013;6:32-6.
2. Groop L, Forsblom C, Lehtovirta M. Characterization of the prediabetic state. Am J Hypertens 1997;10:172-80.
3. Perfetti R, Barnett PS, Mathur R, Egan JM. Novel therapeutic strategies for the treatment of type 2 diabetes. Diabetes Metab Rev 1998;14:207-25.
4. Alexander R. Maltodextrins: production, properties and applications. In: Schenk F, Hebeda R. ed. Starch hydrolysis products; worldwide technology: production and applications, New York; 1992. p. 62–122.
5. Dineshkumar B, Mitra A, Manjunatha M. A comparative study of alpha-amylase inhibitory activities of common antidiabetic plants of Kharagpur 1 block. Int J Green Pharm 2010;4:115-21.
6. Cheng AYY, FantusI G. Oral antihyperglycemic therapy for type 2 diabetes mellitus. Canadian Med Assoc J 2005;172:213–26.
7. Grover JK, Yadav S, Vats V. Medicinal plants of India with anti-diabetic potential. J Ethnopharmacol 2002;81:81–100.
8. Mukherjee PK, Maiti K, Mukherjee K, Houghton PJ. Leads from Indian medicinal plants with hypoglycemic potentials. J Ethnopharmacol 2006;106:1–28.
9. Sunil K, Rashmi Kumar D. Evaluation of antidiabetic activity of Euphorbia hirta Linn. in streptozotocin-induced diabetic mice. Indian J Nat Prod Resour 2010;1:200-3.
10. Rathi Sre PR, Sheila T, Kandasamy M. Phytochemical screening and "in vitro" antioxidant activity of methanolic root extract of Erythrinaindica. Asian Pac J Trop Biomed 2012;2:1696-700.
11. Udayan PS. Tinosporasinensis (Lour.) Merr. From sickupara, kollohills forest, namakkal district tamilnadu. Zoo’s Print J 2004;19:1622-3.
12. Singleton V L, Rossi JA. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. Am J Enol Vitic 1965;16:144-58.
13. Ordonez AAL, Gomez JG, Vattuone MA, Isla MI. Antioxidant activities of Sechiumedule (Jacq.) swart extracts. Food Chem 2006;97:452-8.
14. Kumaran A, Karunakaran RJ. Antioxidant and free radical scavenging activity of an aqueous extract of Coleus aromaticus. Food Chem 2006;97:109-14.
15. Oyaizu M. Studies on the product on browning reaction prepared from glucose amine. Japan J Nutr 1986;44:307-15.
16. Blois MS. Antioxidant determination by the use of-of a stable free radical. Nature 1958;181:1199-200.
17. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Bio Med 1999;26:1231-7.
18. Ali H, Houghton PJ, Soumyanath A. α-amylase inhibitory activity of some Malaysian plants used to treat diabetes; with particular reference to phyllanthusamarus. J Ethnopharmacol 2006;107:449-55.
19. Kim JS, Hyun TK, Kim MJ. The inhibitory effects of ethanol extract from sorghum, foxtail millet and proso millet on 𝛼-glucosidase and 𝛼-amylase activities. Food Chem 2011;124:1647–51.
20. Shai LJ, Masoko P, Mokgotho MP, Magano SR, Mogale MA, Boaduo N, et al. Yeast alpha-glucosidase inhibitory and antioxidant activities of six medicinal plants collected in Phalaborwa. South Afr J Bot 2010;76:465-70.
21. Chandrashekharaiah KS, Menaka N Bolaki, Gupta Shivani Sanjay, Aanchal Bathija, Murthy VK, Narayanaswamy M, Swamy NR. Anti-oxidant and anti-hyperglycemic properties of methanolic extracts of medicinal plants. Biosci Biotech Res Asia 2013;10:607-12.
22. Florence OJ, Adeolu AA, Anthony JA. Comparison of the nutritive value, antioxidant and antibacterial activities of Sonchus asper and Sonchus oleraceus. Rec Nat Prod 2011;5:29-42.
23. Pourmorad F, Hosseinimehr SJ, Shahabimajd N. Antioxidant activity, phenol and flavonoid contents of some selected Iranian medicinal plants. Afr J Biotechnol 2006;5:1142-5.
24. Koleva II, Van Beek TA, Linssen JPH, Groot AD, Evstatieva LN. Screening of plant extracts for antioxidant activity: a comparative study on three testing methods. Phytochem Anal 2002;13:8-17.
25. Paloma Michelle de Sales, Paula Monteiro de Souza, Luiz Alberto Simeoni, Pérola de Oliveira Magalhães, Dâmaris Silveira. α-amylaseinhibitors: a review of raw material and isolated compounds from a plant source. J Pharm Pharm Sci 2012;15:141–83.
26. Kunyanga CN, Imungi JK, Okoth MW, Biesalski HK, Vadivel V. Total phenolic content, antioxidant and antidiabetic properties of methanolic extract of raw and traditionally processed Kenyan indigenous food ingredients. LWT-Food Sci Technol 2012;45:269-76.
27. Mai TT, Thu NN, Tien PG, Van Chuyen N. Alpha-glucosidase inhibitory and antioxidant activities of Vietnamese edible plants and their relationships with polyphenol contents. J Nutr Sci Vitaminol (Tokyo) 2007;53:267–76.
28. Ramkumar KM, Thayumanavan B, Palvannan T, Rajaguru P. Inhibitory effect of Gymnema Montana leaves on α-glucosidase activity and α-amylase activity and their relationship with polyphenolic content. Med Chem Res 2010;19:948-61.
29. Zhenhua Yin, Wei Zhang, Fajin Feng, Yong Zhang, Wenyi Kang. α-Glucosidase inhibitors isolated from medicinal plants. Food Sci Human Wellness 2014;3:136-74.
30. Manikandan R, Vijaya A, Muthumani GD. Phytochemical and in vitro anti-diabetic activity of methanolic extract of Psidiumguajavaleaves. Int J Curr Microbiol Appl Sci 2013;2:15-9.
538 Views | 2752 Downloads
How to Cite
Banerjee, A., B. Maji, S. Mukherjee, K. Chaudhuri, and T. Seal. “IN VITRO ANTI-DIABETIC AND ANTI-OXIDANT ACTIVITIES OF ETHANOL EXTRACT OF TINOSPORA SINENSIS”. International Journal of Current Pharmaceutical Research, Vol. 9, no. 2, Mar. 2017, pp. 42-47, doi:10.22159/ijcpr.2017v9i2.17379.
Original Article(s)