UV-VISIBLE SPECTROSCOPIC AND FTIR ANALYSIS OF SARCOSTEMMA BREVISTIGMA, WIGHT. AND ARN

S. M. Dhivya; K. Kalaichelvi

doi:10.22159/ijcpr.2017.v9i3.18890

S. M. Dhivya and Research Department of Botany, Vellalar College for Women (Autonomous), Thindal, Erode, Tamil Nadu, India 638012
K. Kalaichelvi PG and Research Department of Botany, Vellalar College for Women (Autonomous), Thindal, Erode, Tamil Nadu, India 638012

DOI https://doi.org/10.22159/ijcpr.2017.v9i3.18890

Abstract

Objective: Â The present study was carried out to characterize the bioactive constituents present in aerial parts of ethanolic extract of Sarcostemma brevistigma using UV-VIS and FTIR.

Methods: Â FTIR spectroscopy is an established timeâ€saving method to characterize and identify functional groups.

Results: Â The UV-VIS profile showed different peaks ranging from 200â€1044 nm with different absorption respectively. UV-VIS profile showed the peaks at 254.00 and 680.00 nm for flavonoid. FTIR spectra showed the peak at 3418.85 cm-1 for N-H group. FTIR analysis and UV-VIS analysis showed the presence of flavonoids and a phenolic compound. The FTIR spectra had amply evidenced the occurrence of OH group together with the Terpenoids, and Phenol. The FT-IR spectrum showed the presence of an amine (N-H), alkyl (C-H), nitrile (Câ‰¡N), halo formyl (C=O), alkenyl (C=C), hydroxyl (O-H), haloalkane (C-F), nitrates and carbonate compounds.

Conclusion: Â The results confirm the fact that this plant posseâ€™s important bioactive constituent, so further scientific investigation is needed.

Keywords: UV-Vis Spectrum, FTIR, Sarcostemma brevistigma, fingerprint and functional groups

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