UV-VISIBLE SPECTROSCOPIC AND FTIR ANALYSIS OF SARCOSTEMMA BREVISTIGMA, WIGHT. AND ARN
Abstract
Objective: Â The present study was carried out to characterize the bioactive constituents present in aerial parts of ethanolic extract of Sarcostemma brevistigma using UV-VIS and FTIR.
Methods:  FTIR spectroscopy is an established timeâ€saving method to characterize and identify functional groups.
Results:  The UV-VIS profile showed different peaks ranging from 200â€1044 nm with different absorption respectively. UV-VIS profile showed the peaks at 254.00 and 680.00 nm for flavonoid. FTIR spectra showed the peak at 3418.85 cm-1 for N-H group. FTIR analysis and UV-VIS analysis showed the presence of flavonoids and a phenolic compound. The FTIR spectra had amply evidenced the occurrence of OH group together with the Terpenoids, and Phenol. The FT-IR spectrum showed the presence of an amine (N-H), alkyl (C-H), nitrile (C≡N), halo formyl (C=O), alkenyl (C=C), hydroxyl (O-H), haloalkane (C-F), nitrates and carbonate compounds.
Conclusion: Â The results confirm the fact that this plant posse's important bioactive constituent, so further scientific investigation is needed.
References
2. Garro LC. Intercultural variation folk medicinal knowledge: a comparison between curers and noncurers. American Anthropologist 1986;88:351â€70.
3. Aysal P, Ambrus AD, Lehotay SJ, Cannavan A. Validation of an efficient method for the determination of pesticide residues in fruits and vegetables using ethyl acetate for extraction. J Environ Sci Heal 2007;42:481-90.
4. Grube M, Muter O, Strikauska S, Gavare M, Limane B. Application of FTâ€IR spectroscopy for control of the medium composition during the biodegradation of nitro aromatic compounds. J Indian Microbiol Biotechnol 2008;35:1545–9.
5. Gunasekaran S. UV-VIS spectroscopic analysis of blood serum. Asian J Microbiol Biotech Environ Sci 2003;5:581-2.
6. Dahiya K, Verma M, Dhankhar R., Singh V, Ghalaut PS, Seth S. Alteration of ischemia modified albumin and nitric oxide levels in hypothyroidism. Clin Lab 2014;60:969-72.
7. Ibrahim M, Hameed AJ, Jalbout A. Molecular spectroscopic study of river nile sediment in the greater cairo region. Appl Spectroscopy 2008;62:306-11.
8. Komal Kumar J, Devi Prasad AG. Identification and comparison of biomolecules in medicinal plants of Tephrosia tinctoria and Atylosia albicans by using FTIR. Romanian J Biophys 2011;21:63-71.
9. Hashimoto A, Kameoka T. Applications of infrared spectroscopy to biochemical, food, and agricultural processes. Appl Spectrosc Rev 2008;43:416-51.
10. Sahu N, Saxena J. Phytochemical analysis of Bougainvillea glabra, Choisy. By FTIR and UV-VIS Spectroscopic analysis. Int J Pharm Sci Rev Res 2013;21:196-8.
11. Skoog A, Holler EJ, Crouch SR. Principles of instrumental analysis. 6 Edition; 2007. p. 1039.
12. Starlin T, Arul Raj C, Ragavendran P, Gopalakrishnan VK. Phytochemical screening, functional groups and element analysis of Tylophora pauciflora Wight and Arn. Int Res J Pharm 2012;3:180-3.
13. Maobe MAG, Nyarango RM. Fourier transformer infra-red spectrophotometer analysis of Warburgia ugandensis medicinal herb used for the treatment of Diabetes, Malaria and Pneumonia in Kisii Region, Southwest Kenya. Global J Pharmacol 2013;7:61-8.