• Km. Rubab Anjum
  • K. Sivakumari
  • K. Ashok
  • S. Rajesh


Objective: To study and to explore the fungal species present in indoor toilet environment.

Methods: Lactophenol cotton blue staining.

Results: 5 fungi isolates belonging to 5 genus were identified namely Aspergillus niger, Aspergillus fumigates, Aspergillus flavus, Aspergillus terreus and Rhizopus oryzae.

Conclusion: In order to improve the quality of indoor toilet environment regular cleaning and disinfection should be done daily.

Keywords: Lactophenol cotton blue staining and Aspergillus species


1. Samet JM, Spengler JD. Indoor environments and health: Moving into the 21st centuary. Am J Public Health 2003;93:1489-93.
2. Jarvis WR, Jarvis AA, Chinn RY. National prevalence of methicillin-resistant Staphylocossus aureus in inpatients at the United States health care facilities. Am J Infection Control 2012;40:194-200.
3. Bush RK. Aerobiology of pollen and fungal allergens. J Allergy Clin Immunol 1989;84:1120-4.
4. Hoppe PR. Indoor climate. Experientia 1993;49:775-9.
5. Stark PC. Fungal levels in the home and allergic rhinitis by 5 y of age. Environ Health Perspectives 2005;113:1405-9.
6. Larson EL, Morton HE. Disinfection, sterilization and preservation. Alcohols. Ed. Block SS. Lea and Febiger, Philadelphia; 1991. p. 91.
7. Dacarro C, Picco AM, Grisoli R, Redolfi M. Determination of aerial microbiological contaminations in scholastic sports environment. J Appl Microbiol 2003;95:904-12.
8. Newson R, Strachan D, Corden J, Millington W. Fungal and other spore counts as predictors of admission for asthma in the Trent region. Occup Environ Med 2000;57:786-92.
9. Ross MA, Curtis L, Scheff PA, Hryhorczyk DO, Ramakrishnan V, Wadden RA, et al. Association of asthma symptoms and severity with indoor bioaerosols. Allergy 2000;55:705-11.
10. Gorny RL, Dutkiewicz J. Bacterial and fungal aerosols in indoor environment in Central and Eastern European countries. Ann Agric Environ Med 2002;9:17-23.
11. Cox CS, Waters CM. Bioaerosols handbook. Lewis Publishers, New York; 1995.
12. Muszyński A, Czerwińska A, Chruślińska I. Ocena jakości powietrza wewnętrznego pod względem mikrobiologicznym wpomieszczeniach dydaktycznych Wydziału Inżynierii Środowiska Politechniki Warszawskiej. Warsaw; 1992.
13. Lugauskas A, Krikštaponis A. Microscopic fungi found in libraries of Vilnius and factors affecting their development. Indoor Built Environ 2004;13:169-82.
14. Cheesbrough M. District laboratory practice in Tropical countries. Part 2 Cambridge Low price Editions; 2000. p. 3-8.
15. DeHoog GS, Guarro J, Gene J, Figueras MJ. Atlas of clinical fungi. Ed. 2. Centraal¬bureall voor Schiielcultures/Universitat Rovira i Virgili; 2000.
16. Trudeau W, Fernandes-Caldas E. Identifying and measuring indoor air biologic agents. J Allergy Clin Immunol 1994;94: 393–400.
17. Topbas M, Tosun I, Can G, Kaklikkaya N, Aydin F. Identification and seasonal distribution of airborne fungi in urban outdoor air in an eastern Black Sea Turkish town. Turk J Med Sci 2006;36:31–6.
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How to Cite
Anjum, K. R., K. Sivakumari, K. Ashok, and S. Rajesh. “A PILOT INVESTIGATION INTO ASSOCIATIONS BETWEEN VARIOUS INDOOR AIRBORNE FUNGAL PARTICLES IN TRIPLICANE PUBLIC TOILET OF CHENNAI, TAMIL NADU”. International Journal of Current Pharmaceutical Research, Vol. 9, no. 3, May 2017, pp. 50-53, doi:10.22159/ijcpr.2017.v9i3.18891.
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