IN VITRO EVALUATION OF ANTIMICROBIAL ACTIVITY OF MICRO PROPAGATED CALLUS OF CURUULIGO ORCHIODES (BLACK MUSILLI) AGAR WELL DIFFUSION AND MINIMUM INHIBITORY CONCENTRATION (MIC)


Rajanikanth Garapati, N. Ramesh

Abstract


Objective: In vitro investigated the potential of methanol extracts of micro-propagated C. orchiodes in the antimicrobial property against the three gram-negative bacteria, two gram-positive and one fungal filament.

Methods: The micro propagated callus methanol extract was examined against Escherichia coli, Proteus vulgaris, Salmonella typhimurium, Pseudomonas aeruginosa, Staphylococcus aureus, Bacillus cereus and Candida albicans. The zone of inhibitions are determined at 10 mg/ml concentration of methanol extracts of callus on agar well plate and MIC against tested microorganism.

Results: The highest antibacterial activity recorded in Staphylococcus aureus Bacillus cereus and followed by Candida albicans. Antibacterial activity of leaf extracts of A. reticulata was also significant against the tested microorganisms Escherichia coli, Salmonella typhi, Proteus vulgaris, Pseudomonas aeruginosa compared to ciprofloxacin.

Conclusion: Based on the above observations, these extracts were further evaluated for their effect on microorganisms causing infections like typhoid fever, urinary tract infections, septicemia, toxic shock syndrome, skin infection, nosocomial infection, arthritis and diarrhoea. The results also suggest that these plants serve a therapeutic purpose in the treatment bacterial infections.


Keywords


Antimicrobial activity, Curculigo orchioides, Micro propagted callus extract, Agar well diffusion method, Minimum inhibitory concentration (MIC).

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References


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About this article

Title

IN VITRO EVALUATION OF ANTIMICROBIAL ACTIVITY OF MICRO PROPAGATED CALLUS OF CURUULIGO ORCHIODES (BLACK MUSILLI) AGAR WELL DIFFUSION AND MINIMUM INHIBITORY CONCENTRATION (MIC)

Keywords

Antimicrobial activity, Curculigo orchioides, Micro propagted callus extract, Agar well diffusion method, Minimum inhibitory concentration (MIC).

DOI

10.22159/ijcpr.2017.v9i3.19581

Date

05-05-2017

Additional Links

Manuscript Submission

Journal

International Journal of Current Pharmaceutical Research
Vol 9, Issue 3, 2017 Page: 75-79

Online ISSN

0975-7066

Statistics

19 Views | 16 Downloads

Authors & Affiliations

Rajanikanth Garapati
Department of PG and Research Biotechnology, J. J. College of Arts and Science, Pudukkottai, Tamil Nadu 622001

N. Ramesh
Department of PG and Research Biotechnology, J. J. College of Arts and Science, Pudukkottai, Tamil Nadu 622001


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