PHYTOCHEMICAL EVALUATION OF PUNICA GRANATUM L. LEAF EXTRACT
Objective: This study was conducted to assess the phytochemical constituents in Punica granatum L. Leaf extracts (PGLE) using standard methods.
Methods: The leaf powder was extracted using solvents namely aqueous, hydroalcohol, ethanol, ethyl acetate and n-hexane. Qualitative and Quantitative phytochemical screenings of PGLE were assessed by standard methods.
Results: All the leaf extracts were positive for a wide range of bio-active compounds except n-hexane. The result has showed that the maximum amount of total phenols (394.16 mg/g DW of extract), total tannins (210.5 mg/g DW of extract), flavanoids (147.4 mg/g DW of extract) and total triterpenoids (112 mg/g DW of extract) were noted in ethanolic extract of P. granatum leaf (EPGL). The biological assay revealed that relevant amount of carbohydrate, protein, lipid and alkaloid in EPGL.
Conclusion: The findings of this study concluded that the EPGL had potential bioactive substances that may be used as pharmaceutical ingredients for formulation of new or prospective potent drug to cure wide range of metabolic diseases.
2. Lingarao M, Savithramma N. Phytochemical studies of svensonia hyderobadensis (Walp.) Moldâ€“arare medicinal plant: Der Pharm Lett 2011;3:51-5.
3. Sodipo OA, MA Akanji, FB Kolawole, Odutuga AA. Saponin is the active antifungal principle in Garcinia kola, heckle seed. Biosci Res Commun 1991;3:171-1.
4. Mc Mahon JB, Currens MJ, Gulakowski RJ, Buckheit RWJ, Lackman-Smith C, Hallock YF, et al. Michellamine B a novel plant alkaloid, inhibits human immunodeficiency virus-induced all killing by at least two distinct mechanisms. Antimicrobial Agents Chemother 1995;39:484-8.
5. Noble RI. The discovery of Vinca alkaloids chemotherapeutic agents against cancer: Biochem. Cell Biol 1990;68:1544â€“51.
6. Prasanth Tiwari, Bimlesh Kumar, Mandeep Kaur, Gurpreet Kaur, Harleen Kaur. Phytochemical screening and extraction: review. Int Pharm Sci 2011;1:98-106.
7. Das K, Tiwari RKS, Shrivastava DK. Techniques for evaluation of medicinal plant products as antimicrobial agent: current methods and future trends. J Med Plant Res 2010;4:104-11.
8. Egharevba HO, Kunle OF. Preliminary phytochemical and proximate analysis of the leaves of Piliostigma thionningii (Schumach.): milne-redhead. Ethnobot Leaflets 2010;14:570-7.
9. Naqvi SA, Khan MS, Vohora SB. Antibacterial, antifungal, and antihelminthic investigations on Indian medicinal plants: Fitoterapia 1991;62:221-8.
10. Jayaprakasha GK, Negi PS, Jena BS. Antimicrobial activities of pomegranates, in pomegranates; ancient roots to modern medicines, Eds., CRC Press: Boca Raton, FL, USA; 2006. p. 167-8.
11. Singh RP, Chidambara MKN, Jayaprakasha GK. Studies on the antioxidant activity of pomegranate (Punica granatum) peel and seed extracts using in vitro models: J Agric Food Chem 2002;50:81-6.
12. Sofowara A. Screening plants for bioactive agents. In: Medicinal plants and traditional medicinal in Africa. 2nd edition. Spectrum Book Ltd, Sunshine House, Ibadan Nigeria; 1993. p. 134-56.
13. Trease GE, Evans WC. Pharmacognosy. 15th ed. Sounders Publishers. London; 2002. p. 42-44, 22-229, 246-249, 304-306, 331-332, 391-393.
14. Singleton VL, Rossi JA. Colorimetry of total phenolic with phosphomolybdic acid-phosphotungstic acid reagents. Am J Enol Viticulture 1965;16:144-58.
15. Vishnoi NR. Advanced practical chemistry ghaziabadâ€“India; Yikas Publication House, Pvt Ltd; 1979. p. 447â€“9.
16. El-falleh W, Hannachi H, Tlili N, Yahia Y, Nasri N, Ferchichi A. Total phenolic contents and antioxidant activities of pomegranate peel, seed, leaf and flower. J Med Plants Res 2012;6:4724-30.
17. Peri C, C Pompei CJ. Estimation of different phenolic groups in vegetable extracts. Phytochemistry 1971;19:2187-9.
18. Samantha T, Shyamsundarachary R, Srinivas, P, Swamy NS. Quantification of total phenolic and total flavonoid contents in extracts of oroxylum indicum L. Kurtz. Asian J Pharm Clin Res 2012;5:177-9.
19. Patel AN, Bandawane DD, Mhetre NK. Pomegranate (Punica granatum Linn.) leaves attenuate disturbed homeostasis and hyperglycemia mediated hyperlipidemia and oxidative stress in streptozotocin induced diabetic rats. Eur J Integrative Med 2014;6:307-21.
20. Lu ZM, Gong Js, He Z, Xu HY, Dou WF, Shi JS, et al. Optimization of extraction of total triterpenoids from submergedly cultivated Antrodia Camphorata using response surface methodology. Nat Prod Res Dev 2011;23:946-51.
21. Hedge JE, Hofreiter BT. In: Methods in carbohydrates chemistry. Vol. Eds. Whistler RL, BeMiller JN. Academic Press: New York; 1962. p. 17:.420.
22. Lowry OH, Roseobrough NJ, Farr AL, Randall RJ. Protein measurement with folin phenols reagent. J Biol Chem 1957;93:265-75.
23. Cheung PCK, Leung AYH, Ang PO. Comparison of supercritical carbon dioxide and Soxhlet extraction of lipids from a brown seaweed, Sargassum hemiphyllum (Turn.) C: Ag J Agric Food Chem 1998;46:4228-4232.
24. Harborne JB. Phytochemical methods, London. Chapman and Hall, Ltd; 1973. p. 49-188.
25. Clark TE, Appleton CC, Drewes SE. A semi-quantitative approach to the selection of appropriate candidate plant molluscicidesâ€“a South African application. J Ethnopharmacol 1997;56:1-13.
26. Marston A, Maillard M, Hostettmann K. Search for antifungal, molluscicidal and larvicidal compounds from African medicinal plants. J Ethnopharmacol 1993;38:215-23.
27. Adewunmi CO, Adesina SK, Marquis VO. On the laboratory and field evaluation of the molluscicidal properties of tetrapleura tetraptera. Bull Anim Hlth Prod Afr 1982;30:89-94.
28. Pande G, Akoh CC. Antioxidant capacity and lipid characterization of six georgia-grown pomegranate cultivators. J Agric Food chem 2009;57:9427-36.
29. Hemayat Hossain, Tanzir Ahmed, Sariful Islam Howlader, Shubhra kanti Dey, Arpana Hira, Arif ahmed, et al. In vitro antioxidant potential from the leaves of punica granatum Linn. Grown in Bangladesh. Int J Pharm Phytopharmacol Res 2012;2:160-6.
30. Mir MA, Sawhney SS, Jassal MMS. Qualitative and quantitative analysis of phytochemicals of taraxacum officinale. Wudpecker J Pharm Pharmocol 2013;2:1-57.
31. Stevens JF, Hart HT, Hendriks H, Malingre TM. Alkaloids of some European and macaronesian diode and semepervivodeae (Crassulaceae). Phytochemistry 1992;31:3917-24.
32. Asha K, Rasika CT, Nirmala RD, Jyoti PS. Antioxidant potential from Stem Bark of Juglans regia L. Ann Biol Res 2011;2:176-80.
33. Igboko DO. Phytochemical studies on Garcinia Kola Heckel. M.Sc. Thesis. University of Nigeria, Nsukka; 1983. p. 202.
34. Maiduyi I. Biochemical and pharmacological studies of active principles of the seeds of Garcinia kola Heckel; MSc. Thesis, University of Nigeria, Nsukka; 1983. p. 108.
35. Guangyi Wang, Weiping Tang, Robert R, Bidigare. Terpenoids as therapeutic drugs and pharmaceutical agents: Natural Products; 2005. p. 197-227.
36. McGarvey DJ, Croteau R. Terpenoid metabolism. Plant Cell 1995;7:1015-26.
37. Kam PC, Liew A. Traditional Chinese herbal medicine and anaesthesia. Anaesthesia 2002;57:1083-9.
38. Ayitey-Smith E, Addae-Mensah I. Phytochemical, nutritional and medical properties of some leafy vegetables consumed by Edo people of Nigeria. W Afr J Pharmacol Drug Res 1977;4:7-8.