ANTIBACTERIAL ACTIVITY OF SYNTHESIZED SILVER NANOPARTICLES BY SIMAROUBAGLAUCA AGAINST PATHOGENIC BACTERIA


C. P. Chandrappa, N. Chandrasekar, M. Govindappa, Chaitra Shanbhag, Uttam Kumar Singh, Jayashri Masarghal

Abstract


Objective: The present study outline the plant-mediated synthesis of silver nanoparticles (AgNPs) using leaf extract Simaroubaglauca, which act as both reducing and stabilizing agent.

Methods: Formation of silver nanoparticles was confirmed by primarily by Ultraviolet/visible spectroscopy. X-ray diffraction studies revealed the crystallinity of the nanoparticles. The scanning electron microscopy was carried out to determine the mean particle size, as well as the morphology of the NPs and the composition of elements, was studied with Energy Dispersive X-ray analysis (EDS).

Results: The silver nanoparticles were spherical in shape with a mean size of 23 nm. The EDS showed strong optical absorption peak at 3keV and it was confirmed the formation of AgNPs. The synthesised AgNPs further utilized for the evaluation of antibacterial activity and shown significant antibacterial activity against Escherichia coli, Pseudomonas aeruginosa, Enterobacter and Klebsiella pneumonia at 50 µg/ml and 100µg/ml concentrations.

Conclusion: The synthesised silver nanoparticles have been characterised by UV-vis, SEM-EDAX and XRD to determine the sizes and shapes of the silver nanoparticles.


Keywords


Simaroubaglauca, Silver nanoparticles, Leaf extract, Bioreductant, Antibacterial

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About this article

Title

ANTIBACTERIAL ACTIVITY OF SYNTHESIZED SILVER NANOPARTICLES BY SIMAROUBAGLAUCA AGAINST PATHOGENIC BACTERIA

Keywords

Simaroubaglauca, Silver nanoparticles, Leaf extract, Bioreductant, Antibacterial

DOI

10.22159/ijcpr.2017v9i4.20629

Date

14-07-2017

Additional Links

Manuscript Submission

Journal

International Journal of Current Pharmaceutical Research
Vol 9, Issue 4, 2017 Page: 19-22

Online ISSN

0975-7066

Statistics

132 Views | 57 Downloads

Authors & Affiliations

C. P. Chandrappa
Department of Biotechnology, Shridevi Institute of Engineering and Technology, Sira Road, Tumkur 572106, Karnataka, India

N. Chandrasekar
Department of Chemistry, Shridevi Institute of Engineering and Technology, Sira Road, Tumkur 572106

M. Govindappa
Department of Chemistry, Shridevi Institute of Engineering and Technology, Sira Road, Tumkur 572106

Chaitra Shanbhag
Department of Biotechnology, Shridevi Institute of Engineering and Technology, Sira Road, Tumkur 572106, Karnataka, India

Uttam Kumar Singh
Department of Biotechnology, Shridevi Institute of Engineering and Technology, Sira Road, Tumkur 572106, Karnataka, India

Jayashri Masarghal
Department of Biotechnology, Shridevi Institute of Engineering and Technology, Sira Road, Tumkur 572106, Karnataka, India


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