DEVELOPMENT AND VALIDATION OF A HPTLC METHOD FOR SIMULTANEOUS DENSITOMETRIC ANALYSIS OF GLYCYRRHETIC ACID AND SOLASODINE IN HERBAL DRUG FORMULATION


Kinjal Patel, Chaitanya Bhatt

Abstract


Objective: To develop and validate a simple, precise, selective, and accurate high-performance thin layer chromatographic method for simultaneous densitometric analysis of glycyrrhetic acid and solasodine in the polyherbal formulation.

Methods: The method was developed using HPTLC silica gel GF254precoated aluminium plate as the stationary phase and Chloroform: Methanol (9:1 v/v) as the mobile phase. Quantization of glycyrrhetic acid was achieved by determining the area under the curve at 267 nm using CAMAG TLC Scanner and CATS 3 software. Since the structure of solasodine lacks conjugated double bond, it does not give any fluorescence either in the absorbance mode or reflectance mode hence solasodine was derivatized using 0.5%anisaldehyde sulphuric acids which gave a bluish spot as seen on TLC plate. These spots were scanned at 546 nm wavelength using CAMAG TLC Scanner and CATS 3 software.

Results: The retention factor for glycyrrhetic acid and solasodine were found to be 0.52±0.01, 0.40±0.01% w/w respectively. The developed HPTLC method was validated using parameters described in International Conference on Harmonization (ICH) guideline. The proposed method showed good linearity in the range of 400-2000 ng spot-1 for glycyrrhetic acid as well as for solasodine. The content of glycyrrhetic acid and solasodine in marketed polyherbal formulation were found to be 0.67%±0.8 and 0.10±0.35%w/w respectively.

Conclusion: The developed method can be used as quality control tool for the routine analysis of glycyrrhetic acid and solasodine in polyherbal formulation.


Keywords


Glycyrrhetic acid, Solasodine, HPTLC, Simultaneous estimation

| PDF | HTML |

References


Tolstikov GA, Baltina LA, Serdyuk NG. Glycyrrhetic acid: a review. Pharm Chem J 1998;32:402–12.

Ram A, Bhattacharya I, Das M, Ghosh B, Gangal SV. Evaluation of glycyrrhizin, a compound isolated from Glycyrrhiza glabra for the anti-asthmatic activity using a mouse model of asthma. Indian J Aerobiol 2001;14:42.

Subharani K. Chemical constituents and medicinal properties of Solanumxanthocarpum: a review. Int J Sci Res 2016;5:824-6.

Bector NP, Puri AS. Solanum Xanthocarpum (Kantakari) in chronic bronchitis, bronchial asthma and non-specific unproductive cough. J Assoc Physicians India 1971;19:741-4.

Rathee P, Rathee S, Ahuja D. Simultaneous quantification of glycyrrhetinic acid and apigenin using HPTLC from Glycyrrhiza glabra Linn. Eurasian J Anal Chem 2010;5:95-103.

Trivedi A, Mishra SH. A simple and rapid method for simultaneous estimation of glycyrrhetinic acid and piperine by HPTLC in the herbomineral formulation. J Adv Pharm Tech Res 2010;1:190-8.

Vampa G, Benvenuti S. Separation of 18α-and 18ß-glycyrrhetinic acid by high-performance thin-layer chromatographic densitometry. J Chromatography 1991;543:479-82.

Esmaeili S, Naghibi F, Mosaddegh M, Nader N. Determination of 18 ß-glycyrrhetinic acids in Glycyrrhiza glabra L. extract by HPLC. Iranian J Pharm Res 2006;5:137-41.

Zhou S, Zhang X, Duan X, Mei H. HPLC determination of glycyrrhizic acid andglycyrrhetinic acid in weiyanning granules. Biomed Res 2015;26:311-4.

Shanker K, Gupta S, Shrivastava P, Shrivastava SK, Sing SC, Gupta MM. Simultaneous determination of three steroidal glycoalkaloids in Solanum xanthocarpum by high-performance thin layer chromatography. J Pharma Biomed Anal 2011;54:497–502.

Trivedi P, Pundarikakshudu K. Novel TLC densitometric method for quantification of solasodine in various Solanum species, market samples and formulations. Chromatogr 2006;65:239–43.

Kittipongpatana N, Porter JR, Hock RS. An improved high performance liquid chromatographic method for the quantification of solasodine. Phytochem Anal 1999;10:26–31.

Trivedi PD, Patel BC, Rathnam SB, Pundarikakshudu K. A validated a liquid chromatographic method for estimation of solasodine in various Solanum species and market formulations. J AOAC Int 2006;89:1519-23.

ICH Harmonised Tripartite Guideline, Validation of Analytical Procedures: Text and Methodology Q2 (R1), Nov; 2005.




About this article

Title

DEVELOPMENT AND VALIDATION OF A HPTLC METHOD FOR SIMULTANEOUS DENSITOMETRIC ANALYSIS OF GLYCYRRHETIC ACID AND SOLASODINE IN HERBAL DRUG FORMULATION

Keywords

Glycyrrhetic acid, Solasodine, HPTLC, Simultaneous estimation

DOI

10.22159/ijcpr.2017v9i4.20752

Date

14-07-2017

Additional Links

Manuscript Submission

Journal

International Journal of Current Pharmaceutical Research
Vol 9, Issue 4, 2017 Page: 29-32

Online ISSN

0975-7066

Statistics

63 Views | 37 Downloads

Authors & Affiliations

Kinjal Patel
Department of Pharmacognosy, Shree Swaminarayan Sanskar Pharmacy College, Zundal Ghandhinagar, India

Chaitanya Bhatt
Department of Pharmacognosy, Shree Swaminarayan Sanskar Pharmacy College, Zundal Ghandhinagar, India


Refbacks

  • There are currently no refbacks.