• Prabu M. Department of Biology, Gandhigram Rural Institute-Deemed University, Gandhigram 624302 Dindigul (TN), India
  • Kumuthakalavalli R. Department of Biology, Gandhigram Rural Institute-Deemed University, Gandhigram 624302 Dindigul (TN), India



Pleurotus florida, Phenols, Flavonoids, Antidiabetic activity, Antidiabetic agent


Objective: The present investigation comprises, in vitro antidiabetic activity such as α-amylase and α-glucosidase inhibitory activities and in vivo antidiabetic activity of methanolic extract of Pleurotus florida.

Methods: The fruiting bodies of Pleurotus florida were obtained from Mushroom Unit, Department of Biology, Gandhigram Rural Institute-Deemed University, Gandhigram, Dindigul, Tamil Nadu, India. Sample preparation, qualitative phytochemical analysis, in vitro antidiabetic activities namely α-amylase and α-glucosidase inhibitory activity and in vivo antidiabetic activity namely evaluation of alloxan induced diabetic rats were carried out following the methods reported previously.

Results: In vitro and in vivo antidiabetic activity of P. florida exhibited significant results for its α-amylase (94.93±1.75 % at 1000 µg/ml) and α-glucosidase inhibitory activity (84.90±0.42 % at 1000 µg/ml) in a dose-dependent manner. The extract also showed significant antidiabetic activity on in vivo (p<0.05) at the tested dose level (200 mg/kg b. w) this was comparable to Glibenclamide, a standard antidiabetic drug.

Conclusion: The presence of phytochemicals namely phenols, flavonoids, saponins, tannins and terpenoids may be responsible for such antidiabetic activity. These results reveal that P. florida can be used as a potential antidiabetic agent.


Zimmet P, Alberti G, Shaw J. Global and societal implications of the diabetes epidemic. Nature 2001;414:782-7.

World Health Organization. Traditional Medicinal Strategy 2002-2005. WHO Publications; 2002. p. 1-6.

Li WL, Zheng HC, Bukuru J, Kimpe ND. Natural medicines used in the traditional Chinese medical system for therapy of diabetes mellitus. J Ethnopharmacol 2004;92:1-21.

Intekhab A, Barry G. Diabetes mellitus. Clin Dermatol 2006;24:237-46.

Berger W. The incidence of severe side effects during therapy with sulphonylureas and biguanide. Hormones Metabol Res 1985;17:111â€5.

Rang HP, Dale MM, Ritter JM. The endocrine system pharmacology. Longman Group Ltd. (UK); 1991.

Rao BK, Kesavulu MM, Apparao C. Evaluation of the antidiabetic effect of Momordica cynobalaria fruit in alloxan diabetic rats. J Fitoterapia 2003;74:7-13.

Cragg GM, Newmann DJ, Snader KM. Natural products in drug discovery and development. J Nat Prod 1997;60:52–60.

Wong SP, Naidu M, David P, Bakar R, Vikineswary S. Neurogenerative potential of lions mane mushroom, Hericium erinaceus (Bull.: Fr.) Pers., (Higher Basidiomycetes), in the treatment of peripheral nerve injury. Int J Med Mushrooms 2012;14:427–46.

Prakasam V. Current scenario of mushroom research in India. Indian Phytopath 2012;65:1-11.

Prabu M, Kumuthakalavalli R. Antioxidant activity of oyster mushroom (Pleurotus florida [Mont.] Singer) and milky mushroom (Calocybe indica P and C). Int J Curr Pharm Res 2016;8:48-51.

Ngai PH, Ng TB. A Hemolysin from the mushroom Pleurotus eryngii. Appl Microbiol Biotechnol 2006;75:1185-91.

Hu SH, Chia VC, Lien JL, Chen KS, Lee MY, Wang JC. Antihyperlipidaemic and antioxidant effects of extracts of Pleurotus citrinopileatus. J Agric Food Chem 2006;54:2103-10.

Zhang M, Cui SW, Cheung PCK, Wang Q. Antitumor polysaccharides from mushrooms: a review of their isolation process, structural characteristics, and antitumor activity. Trends Food Sci Technol 2007;18:4–19.

DeFronzo R, Ferrannini E, Alberti G, Zimmet P. International Textbook of Diabetes Mellitus. 4th ed. Wiley-Blackwell Publishers, Chichester, UK; 2015.

Packer L, Rfsen P, Tritschler HJ, King GL, Azzi A. Antioxidants in Diabetes Management. Marcel Dekker, New York; 2000.

Silva D, Rapior S, Hyde KD, Bahkali AH. Medicinal mushrooms in prevention and control of diabetes mellitus. Fungal Divers 2012;56:1–29.

Suffness M, Douros J. Drugs of plant origin. In: Methods in Cancer Research, Academic Press; New York. DeVita VT, Busch H. Eds; 1979;26:73–126.

Harborne JB. Phytochemical methods: a guide to modern techniques of plant analysis. Published by Chapman and Hall, an imprint of Thomson Science, London, UK; 1973.

Miller GL. Use of dinitro salicylic acid reagent for determination of reducing sugar. Ann Chem 1959;31:426–8.

Phuong TM, Lee D, Lee KH. Regression trees for regulatory element identification. Bioinformatics 2004;20:750–7.

Cheng A, Fantus I. Oral antihyperglycemic therapy for type 2 diabetes mellitus. Can Med Assoc J 2005;172:213–6.

LoPiparo E, Scheib H, Frei N, Williamson G, Grigorov M, Chou CJ. Flavonoids for controlling starch digestion: structural requirements for inhibiting human α-amylase. J Med Chem 2008;51:3555–61.

Sabitha V, Panneerselvam K, Ramachandran S. In vitro α-glucosidase and α-amylase enzyme, inhibitory effects in aqueous extracts of Abelmoschus esculentus (L.) moench. Asian Pac J Trop Biomed 2012;2:162–4.

Lobovitz H. α-glucosidase inhibitors. Endocrinol Metabol Clin North Am 1997;26:539–51.

Inzucchi SE. Oral antihyperglycemic therapy for type 2 diabetes. Sci Rev 2002;287:360–72.

Laar FA, Lucassen PL, Akkermans RP, Lisdonk EH, Rutten GE, Weel C. α-glucosidase inhibitors for patients with type 2 diabetes: results from a cochrane systematic review and meta-analysis. Diabetes Care 2005;28:166–75.

Thorat K, Patil L, Limaya D, Kadam V. In vitro models for antidiabetic activity assessment. Int J Res Pharm Biomed Sci 2012;3:730–2.

Jumepaeng T, Prachakool S, Luthria DL, Chanthai S. Determination of antioxidant capacity and α–amylase inhibitory activity of the essential oils from Citronella grass and Lemongrass. Int J Food Res 2013;20:481–5.

Bischoff H. Pharmacology of αglucosidase inhibitor. Eur J Clin Invest 1994;24:3-10.

Silva MCM, Grossi-de-Sa MF, Chrispeels MJ, Togawa RC, Neshich G. Analysis of structural and physicochemical parameters involved in the specificity of binding between α-amylases and their inhibitors. Protein Eng 2000;13:167-77.

Ali H, Houghton PJ, Soumyanath A. The α-amylase inhibitory activity of some Malaysian plants used to treat diabetes with particular reference to Phyllanthus amarus. J Ethnopharmacol 2006;107:449-55.

Davis SN, Granner DK. Insulin, oral hypoglycemic activity agents and the pharmacology of endocrine pancreas. In: Goodman and Gilman’s: The pharmacological basis of therapeutics. 11th ed. McGraw-Hill Medical Publication Division: New York. Brunton et al. Eds; 2001. p. 1706–7.

Boivin M, Flourie B, Rizza RA, Go VL, DeMagno EP. Gastrointestinal and metabolic effects of amylase inhibition in diabetics. Gastroenterology 1998;94:387-94.

Kim JS, Kwon CS, Son KH. Inhibition of alpha glucosidase and amylase by luteolin, a flavonoid. Biosci Biotechnol Biochem 2000;64:2458-61.

Lilliooja S, Mott DM, Spraul M, Ferraro R, Foley JE, Ravussin E. Insulin resistance and insulin secretory dysfunction as precursors of non-insulin-dependent diabetes. Prospective Study of Pima Indians. Engl J Med 1993;329:1988-92.

Rajeswari P, Krishnakumari S. The potent antihyperglycemic activity of Calocybe indica in streptozotocin-induced diabetic rat's antihyperglycemic activity of Calocybe indica. Int J Pharm Pharm Sci 2013;5:512–5.

Brownlee M. Biochemistry and molecular cell biology of diabetic complications. Nature 2001;414:813–20.

Evans JL, Goldfine ID, Maddux BA, Grodsky GM. Oxidative stress and stress-activated signalling pathways: a unifying hypothesis of type 2 diabetes. Endocr Rev 2002;23:599–622.

Jay D, Hitomi H, Griendling KK. Oxidative stress and diabetic cardiovascular complications. Free Radical Biol Med 2006;40:183–92.

Kowluru RA, Kowluru A, Kanwar M. Small molecular weight G-protein, H-Ras, and retinal endothelial cell apoptosis in diabetes. Mol Cell Biochem 2007;296:69–76.

Defronzo RA, Ferranini E. Insulin resistance: a multifaceted syndrome responsible for NIDDM, obesity, hypertension, dyslipidemia and atherosclerotic cardiovascular disease. Diabetes Care 1991;14:173–94.

Wi JE, Kim JK, Youn JH. Reduced glucose clearance as the major determinant of postabsorptive hyperglycemia in rats. Am J Physiol Endocrinol Metab 1998;274:257–64.

Krishna J, Pandey B, Pathak DB. Characterization of dichotomoceras in the oxfordian of kachchh. J Geol Soc India 2009;74:469–79.

Khan MA, Rahman MM, Tania M, Uddin MN, Ahmad S. Pleurotus sajor-caju and Pleurotus florida mushrooms improve some extent of the antioxidant systems in the liver of hypercholesterolemic rats. Open Nutraceuticals J 2011;4:20–4.

Prabu M, Kumuthakalavalli R. In vitro and in vivo antidiabetic activity of Calocybe indica P and C. In: 8th International Conference on Mushroom Biology and Mushroom Products. Organized by Mushroom Society of India; 2014. p. 433–7.

Johnny I, Okon J. Antidiabetic effect of Pleurotus ostreatus (Jacq ex Fr) Kumm, mushroom on alloxan induced diabetic rats. Indian J Pharm Biol Res 2013;1:31–6.



How to Cite

M., P., and K. R. “ANTIDIABETIC POTENTIAL OF THE OYSTER MUSHROOM PLEUROTUS FLORIDA (MONT.) SINGER”. International Journal of Current Pharmaceutical Research, vol. 9, no. 4, July 2017, pp. 51-54, doi:10.22159/ijcpr.2017v9i4.20765.



Original Article(s)