STUDIES ON NUTRITIONAL PROFILE AND ANTIOXIDANT POTENTIAL OF DIFFERENT DIOSCOREA SP WITH PLECTRANTHUS ROTUNDIFOLIUS
Objective: Protection from oxidative damage to the tissues is provided by natural or synthetic antioxidants. Because of the multiple mechanisms of actions and presence of various phytochemicals, plant-based antioxidants are preferred. Dioscorea is well known as a traditional edible tuber. The present study was undertaken to explore and highlights the nutritional benefits and to evaluate the in vitro antioxidant and free radical scavenging capacity of ethanolic extract of three different underutilised Dioscorea species (D. alata, D. pentaphylla and D. oppositifolia) with a common cultivated crop, Plectranthus rotundifolius.
Methods: Different processing methods are adopted to assess their influence on nutritive value and antioxidant properties. Tubers were studied for proximate composition using standard analytical methods. Mineral elements were analysed using ICP-MS (Inductively Coupled Plasma-Mass Spectrometer). Antioxidant potential of raw and processed samples was determined using a range of in vitro models.
Results: The presence of essential minerals such as Na, K, Ca, P, Zn and Mn were observed. High radical scavenging activity was observed in the tubers of Dioscorea. Results indicated that phenolic compounds are the significant contributors to the antioxidant activity.
Conclusion: Current study suggests that Dioscorea might be a promising natural antioxidant and could be very useful health food, as they enhance the antioxidant defence system. It also proposes that underutilized tubers can efficiently contribute to the nutritional requirement and food security.Â
2. Pedralli G, Carmo CAS, Cereda M, Puiatti M. Uso de nomes populares para as espÃ©cies de Araceae e Dioscoreaceae no Brasil. Hortic Bras 2002;20:530-2.
3. Pedralli G. Flora ilustrada catarinense. FundaÃ§Ã£o Cultural de ItajaÃ. Santa Catarina; 2004.
4. Nassar N. Cassava in South America, Brazilâ€™s contribution and the lesson to be learned from India. Genet Mol Res 2006b;5:688-95.
5. Nindjin C, OtokorÃ© D, Hauser S, Tschannen A, Farah Z, Girardin O. Determination of relevant sensory properties of pounded yams (Dioscorea spp.) using a locally based descriptive analysis methodology. Food Quality Pref 2007;18:450-9.
6. Hou WC, Mei-Hsien Lee, Chen HJ, Wen-Lee Liang, Han CH, Yen-Wenn Liu, et al. Antioxidant activities of dioscorin, the storage protein of yam (Dioscorea batatas Decne) tuber. J Agric Food Chem 2001;49:4956âˆ’60.
7. Lu YL, Cho-Yun Chia, Liu Y W, Wen-Chi Hou. Biological activities and applications of dioscorins, the major tuber storage proteins of yam. Afr J Tradit Complementary Altern Med 2012;2:41â€“6.
8. Hsu FL, Lin YH, Lee MH, Lin CL, Hou WC. Both dioscorin, the tuber storage protein of yam (Dioscorea alata cv. Tainong Nr 1), and its peptic hydrolysates exhibited angiotensin converting enzyme inhibitory activities. J Agric Food Chem 2002;50:6109â€“13.
9. Kay DE. Root Crops. TPI Crop and Product Digest, Overseas Development Agency, London; 1973.
10. Bayani Uttara, Ajay V Singh, Paolo Zamboni, Mahajan RT. Oxidative stress and neurodegenerative diseases: a review of upstream and downstream antioxidant therapeutic options. Curr Neuropharmacol 2009;7:65â€“74.
11. Young S, Woodside JVJ. Antioxidants in health and disease. Clin Pathol 2001;54:176â€“86.
12. Badarinath AV, Rao KM, Chetty CMS, Ramkanth S, Rajan TVS, Gnanaprakash K. A review on in vitro antioxidant methods: comparisons, correlations and considerations. Int J PharmTech Res 2010;2:1276-8.
13. Rizvi, Kanti Bhooshan Pandey, Syed Ibrahim. Plant polyphenols as dietary antioxidants in human health and disease. Oxid Med Cell Longevity 2009;5:270-8.
14. Ali SS, Kasoju N, Luthra A, Singh A, Sharanabasava H, Sahu A, et al. Indian medicinal herbs as sources of antioxidants. Food Res Inter 2008;41:1-15.
15. Ajith TA, Janardhanan KK. Antioxidant and antihepatotoxic activities of Phellinus rimosus (Berk) pilat. J Ethnopharmacol 2002;81:387-91.
16. Anupam G, Bidus KD, Soroj KC, Goutam C. Antibacterial potentiality and phytochemical analysis of mature leaves of Polyalthialongifolia (Magnoliales: Annonaceae). South Pacific J Natural Sci 2008;26:68-72.
17. Anand PK, Policegoudra RS, Aradhya SM. Chemical composition and antioxidant activity of Sapota (Achrassapotalinn.) fruit. J Food Biochem 2007;31:399â€“414.
18. Magbagbeola JAO, Adetoso JA, Owolabi OA. Neglected and underutilised species (NUS): a panacea for community-focused development to poverty alleviation/poverty reduction in Nigeria. J Economics Int Finance 2010;2:208â€“11.
19. Janick J. Editor. Padulosi S, Eyzaquirre P, Hodgkin T. Challenges and Strategies in Promoting Conservation and Use of Neglected and Underutilized Crop Species. ASHS Press, Alexandria, Va, USA; 1999.
20. Johns T, Eyzaguirre PB. Symposium on â€œwild gathered plants: basic nutrition, health and survivalâ€ Linking biodiversity, diet and health in policy and practice,â€ Proc Nutr Soc 2006;65:182â€“9.
21. Ghane SG, Lokhande VH, Ahire ML, Nikam TD. Indigofera glandulosa Wendl. (Barbada) a potential source of nutritious food: underutilized and neglected legume in India. Genet Resour Crop Evol 2010;57:147â€“53.
22. Frison E, Omont H, Padulosi S. GFAR and international cooperation on commodity chains. synthesis paper for presentation to the GFAR-2000 Conference, Dresden, Germany; 2000. p. 21-23.
23. FAO (Food and Agriculture Organization of the United Nations). Production indices and Pop STAT. FAO, Statistics Division; 2009. Available from: http://faostat.fao.org/default.aspx. [Last accessed on 08 Dec 2009]
24. AOAC. Official Methods of Analysis. 14th ed. Washington DC: Association of Official Analytical Chemists; 1990.
25. Siddhuraju P, Vijayakumari K, Janardhanan K. Chemical composition and nutritional evaluation of an underexploited legume, Acacia nilotica (L.) Del. Food Chem 1996;57:385-91.
26. Yu L, Haley S, Perret J, Harris M, Wilson J, Qian M. Free radical scavenging properties of wheat extracts. J Agric Food Chem 2002;50:1619-24.
27. Makkar HPS, Francis G, Becker K. Bioactivity of phytochemicals in some lesser-known plants and their effects and potential applications in livestock and aquaculture production systems. Animal 2007;1:1371-91.
28. Siddhuraju P, Becker K. Antioxidant properties of various solvent extracts of total phenolic constituents from three different agroclimatic origins of Drumstick tree (Moringa olifera Lam.) leaves. J Agric Food Chem 2003;51:2144-55.
29. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans E. Antioxidant activity applying an improved ABTS radical cation decolonization assay. Free Radical Biol Med 1999;26:1231-7.
30. Blois MS. Antioxidant determination by the use of a stable free radical. Nat 1958;181:1199-200.
31. Brand-Williams W, Cuvelier ME, Berset C. Use of a free radical method to evaluate antioxidant activity. LWT-Food Sci Technol 1995;28:25-30.
32. Pulido R, Bravo L, Saura-Calixto F. Antioxidant activity of dietary polyphenols as determined by a modified ferric reducing/antioxidant power assay. J Agric Food Chem 2000;48:3396-402.
33. Dinis TCP, Madeira VMC, Almeida LM. The action of phenolic derivatives (acetaminophen and as peroxyl radical scavengers. Arch Biochem Biophys 1994;315:161-9.
34. Udensi EA, Oselebe HO, Iweala OO. The investigation of chemical composition and functional properties of water yam (Dioscorea alata): effect of varietal differences. Pak J Nutr 2008;7:342-4.
35. Iheanacho K, Ubebani AC. Nutritional composition of some leafy vegetable consumed in Imo State. JASEM 2009;13:35-8.
36. Kwenin WKJ, Wolli M, Dzomeku BM. Assessing the nutritional value of some African indigenous green leafy vegetables in Ghana. J Anim Plant Sci 2011;10:1300-5.
37. Afoakwa EO, Sefa-Dedeh S. Chemical composition and quality changes occurring in Dioscorea dumetorum pax tubers after harvest. Food Chem 2001;75:85â€“91.
38. Ramirez-Moreno E, Cordoba-Diaz D, de Cortes Sanchez-Mata M, Diez-Marques C, Goni I. Effect of boiling on nutritional, antioxidant and physicochemical characteristics in Cladodes (Opuntia ficus indica). LWT-Food Sci Tech 2013;51:296-302.
39. Huang CC, Mei-Chen Lin, Chiun CR Wang. Changes in morphological, thermal and pasting properties of yam (Dioscorea alata) starch during growth. Carbohydrate Polymers 2006;64:524-31.
40. Oko AO, Famurewa AC. Estimation of nutritional and starch characteristics of Dioscorea alata (water yam) varieties commonly cultivated in the South-Eastern Nigeria. Br J Appl Sci Tech 2015;6:145-52.
41. Wanasundera PKJPD, Ravindran G. Nutritional assessment of yam (Dioscorea alata) tubers. Plant Foods Human Nutr 1994;46:33â€“9.
42. Gupta UC, Gupta SC. Sources and deficiency diseases of mineral nutrients in human health and nutrition: a review. Pedosphere 2014;24:13-38.
43. Demigne C, Sabboh H, Reme SC, Meneton P. Protective effects of high dietary potassium in nutritional and metabolic aspects. J Nutr 2004;134:2903-6.
44. Tapia MI, Sanchez-Morgado JR, Garcia-Parra J, Ramirez R, Hernandez T, Gonzalez-Gomez D. Comparative study of the nutritional and bioactive compounds content of four walnut (Juglans regia L.) cultivars. J Food Compost Anal 2013;31:232-7.
45. Lewu MN, Adebola PO, Afolayan AJ. Comparative assessment of the nutritional value of commercially available cocoyam and potato tubers in South Africa. J Food Quality 2010b;33:461-76.
46. Caidan R, Cairang L, Liu B, Suo Y. Amino acid, fatty acid and mineral compositions of fruit, stem, leaf and root of Rubus amabilis from the Quighai-Tibetan Plateau. J Food Compost Anal 2014;33:26-31.
47. Akpanyung EO. The proximate and mineral composition of bouillon cubes produced in Nigeria. Pak J Nutr 2005;4:327-9.
48. Bhaskaran P. Immunobiology of mild nutrient deficiency. Br J Nutr 2001;85:75-80.
49. Geissler CA, Powers HJ. Human nutrition. Elsevier, Churchill Livingstone 2005;11:236-43.
50. Anyoola PB, Adeyeye A, Onawumi OO. Trace element and a major evaluation of Spondias mombin, Veronia amygdalina and Momordia charantia leave. Pak J Nutr 2010;9:755-8.
51. Njoku PC, Ohia CC. Spectrophotometric estimation studies of mineral nutrient in three cocoyam cultivars. Pak J Nutr 2007;6:616-9.
52. Kenny O, Smyth TJ, Hewage CM, Brunton NP. Antioxidant properties and quantitative UPLC-MS analysis of phenolic compounds from extracts of Fenugreek (Trigonella foenum-graecum) seeds and Bitter melon (Momordica charantia) fruit. Food Chem 2013;141:4295-302.
53. Crozier A, Kamiya Y, Bishop G, Yokota T. Biosynthesis of hormones and elicitor molecules. In: Buchanan BB, Gruissem W, Jones RL. editors. Biochemistry and molecular biology of plants. American Society of Plant Physiologists, Rockville; 2000. p. 850-929.
54. Valdez LB, Alvarex S, Lores AS, Schoopfer F, Carreras MC, Poderoso JJ, et al. Reactions of peroxynitrite in the mitochondrial matrix. Free Radical Biol Med 2000;29:349-56.
55. Sowndhararajan K, Sun Chul Kang. Free radical scavenging activity from different extracts of leaves of Bauhinia vahlii Wight and Arn. Saudi J Biol Sci 2013;20:319-25.
56. Saidu AN, Mann A, Onuegbu CD. Phytochemical screening and hypoglycemic effect of aqueous Blighia sapida root bark extract on normoglycemic albino rats. Br J Pharm Res 2012;2:89-97.
57. Sasikumar JM, Maheshu V, Aseervatham GSB, Darsini DTP. In vitro antioxidant activity of Hedyotis corymbosa (L) Lam aerial plants. Indian J Biochem Biophy 2010;47:49-52.
58. Kumari M, Jain S. Tannins: an antinutrient with positive effect to manage diabetes. Res J Recent Sci 2012;1:70-3.
59. Serrano J, Pupponen-Pimia R, Dauer A, Aura AM, Saura-Calixto F. Tannins: current knowledge of food sources, intake, bioavailability and biological effects. Mol Nutr Food Res 2009;53:S310-S329.
60. Bravo L. Polyphenols: chemistry, dietary sources, metabolism, and nutritional significance. Nutr Rev 1994;56:317-33.
61. Manach C, Morand C, Gil-Izquierdo A. Polyphenols: food sources and bioavailability. Am J Clin Nutr 2004;79:727-47.
62. Mithraja MJ, Johnson Marimuthu Antonisamy, Mony Mahesh, Paul ZM, Solomon Jeeva. Phytochemical studies on Azolla pinnata R. Br., Marsilea minuta L. and Salvinia molesta Mitch. Asian Pacific J Trop Biomed 2011;1:S26-S29.
63. Espley R, Martens S. Health properties of apple and pear, in bioactives in fruit: health benefits and functional foods. Skinner M, Hunter D. editors. John Wiley and Sons Ltd, Oxford, UK; 2013. p. 542.
64. Nithiyanantham S, Selvakumar S, Siddhuraju P. Total phenolic content and antioxidant activity of two different solvent extracts from raw and processed legumes, Cicer arietinum L. and Pisum sativum L. J Food Compost Anal 2012;27:52-60.
65. Eklund PC, Langvik OK, Warna JP, Salmi TO, Willfor SM, Sjoholm RE. Chemical studies on antioxidant mechanisms and free radical scavenging properties of lignans. Org Biomol Chem 2005;3:3336-47.
66. Fauconneau B, Waffo-Teguo P, Huguet F, Barrier L, Decendit A, Merillon JM. Comparative study of radical scavenger and antioxidant properties of phenolic compounds from Vitis vinifera cell cultures using in vitro tests. Life Sci 1997;61:2103-10.
67. Adefegha SA, Oboh G. Cooking enhances the antioxidant properties of some tropical green leafy vegetables by steam cooking. J Food Process Pres 2011;35:615-22.
68. Miller JK, Brzezinska-Slebodzinska E, Madsen FC. Oxidative stress, antioxidants and animal function. J Diary Sci 1993;76:2812-23.
69. Pokorny J, Yanishlieva N, Gordon M. Antioxidants in food practical applications. Wood head publishing Ltd, Abington hall, Abington; 2001. p. 73.
70. Oboh G, Raddatz H, Henle T. Antioxidant properties of polar and non-polar extracts of some tropical green leafy vegetables. J Sci Food Agric 2008;88:2486â€“92.
71. Marghitas LA, Stanciu OG, Dezmirean DS, Bobis O, Popescu O, Bogdanov S, et al. In vitro antioxidant capacity of honeybee-collected pollen of selected floral origin harvested from Romania. Food Chem 2009;115:878-83.
72. Oueslatia S, Trabelsia N, Boulaabaa M, Legaultb J, Abdellya C, Ksouri R. Evaluation of antioxidant activities of the edible and medicinal Suaeda species and related phenolic compounds. Ind Crops Prod 2012;36:513-8.
73. Shakirin FH, Prasad KN, Ismail A, Yuon LC, Azlan A. Antioxidant capacity of underutilized Malaysian Canarium odontophyllum (dabai) Miq. Fruit J Food Compost Anal 2010;23:777-81.
74. Chung YC, Chang CT, Chao WW, Lin CF, Chou ST. Antioxidative activity and safety of the 50% ethanolic extract from red bean fermented by Bacillus subtilis IMR-NK1. J Agric Food Chem 2002;50:2454.
75. Afolabi C, Akinmoladun EO, Ibukun, Emmanuel Afor BL, Akinrinlola TR, Onibon AO, et al. Chemical constituents and antioxidant activity of alstoniaboonei. Afr J Biotech 2007;6:1197-201.
76. Gorinstein S, Jastrzebski Z, Leontowicz H, Leontowicz M, Namiesnik J, Najmanc K. Comparative control of the bioactivity of some frequently consumed vegetables subjected to different processing conditions. Food Control 2009;20:407-13.
77. Dini I, Tenore GC, Dini A. Effect of industrial and domestic processing on antioxidant properties of pumpkin pulp. LWT-Food Sci Technol 2013;53:382-5.
78. Wu SJ, Ng LT. Antioxidant and free radical scavenging activities of wild bitter melon (Momordica charantia linn. var. abbreviate Ser.) in Taiwan. LWT-Food Sci Technol 2008;41:323-30.
79. Loizzo MR, Tundis R, Bonesi M, Menichini F, Mastellone V, Avallone L. Radical scavenging, antioxidant and metal chelating activities of Annona cherimola Mill. (Cherimoya) peel and pulp in relation to their total phenolic and total flavonoid contents. J Food Compost Anal 2012;25:179-84.
80. Khanam UKS, Oba S, Yanase E, Murakami Y. Phenolic acids, flvonoids and total antioxidant capacity of selected leafy vegetables. J Funct Food 2012;4:979-87.