• Doke Suraj Yashwant Department of Quality Assurance, D. S. T. S. Mandal’s College of Pharmacy, Solapur 413004
  • Chavare Pranav Mohan Department of Quality Assurance, D. S. T. S. Mandal’s College of Pharmacy, Solapur 413004
  • Belvotagi Venkatrao Adavirao Department of Quality Assurance, D. S. T. S. Mandal’s College of Pharmacy, Solapur 413004


Objective: A large number of farmers are growing different vegetables and supplying them to the Solapur market. The majority of these farms are located on the banks of the nullahâ€, whose water is used for irrigation. From, a farm field in Degaon, a village on the Solapur–Mangalvedha road and on the bank of ‘nullah' two fruit vegetables viz., Ladies Finger (Bhendi) and Cluster Beans (Gawar) are evaluated in the study. The purpose of this study was to analyse the presence of selected toxic non-essential heavy metals i.e. Arsenic (As), Cadmium (Cd), Chromium (Cr), Lead (Pb) and Mercury (Hg) in the two vegetables.

Methods: Atomic Absorption Spectrophotometer (AAS) was used for determining the concentration of heavy metals. Optimised wet acid digestion procedure was employed to solubilize the metals from the vegetable samples.

Results: The following concentrations (mg/kg) of the non-essential heavy metals were found in the edible parts of the Ladies Finger and Cluster Beans, respectively: Ladies Finger: As (16.07), Hg (16.77), Pb (1.148), Cr (17.14), Cd (0.704 mg/kg) and Cluster Beans: As (18.76), Hg (21.86), Pb (1.675), Cr (17.14), Cd (0.888 mg/kg). The study revealed the presence of all the chosen NEHMs in the vegetables. Cadmium levels in both Cluster Beans and Ladies Finger were below the maximum acceptable limit and hence fit for human consumption as per FSSAI standards As (1.1), Pb (1.0), Cd (1.5), Hg (1.0 ppm). Though unsafe as per WHO/FAO standards As (0.1), Pb (0.5), Cd (0.2), Cr (1.2), Hg (0.05 ppm).

Conclusion: The other three NEHMs levels were approximately 15 times more than the permitted levels and hence might pose danger on human consumption.


Keywords: AAS, Cluster Beans, Grey water, Ladies Finger, Non-essential heavy metals, WHO/FAO, FSSAI


1. Morel A. Greywater treatment on a household level in developing countries–a state of the art review. Semester work of barba raimhof and joëlle mühlemann; 2005. p. 1-98.
2. Lone AH, Lal EP, Thakur S. Accumulation of heavy metals on soil and vegetable crops grown on sewage and tube well water irrigation. Sci Res Essays 2013;8:2187-93.
3. Deribachew B, Made M, Nigussie-Dechassa R, Taddesse AM. Selected heavy metals in some vegetables produced through wastewater irrigation and their toxicological implications in eastern Ethiopia. Afr J Food Agric Nutr Dev 2015;15:10013-32.
4. Akan JC, Kolo BG, Yikala BS, Ogugbuaja VO. Determination of some heavy metals in vegetable samples from biu local government Area, Borno State, North Eastern Nigeria. Int J Environ Monit Anal 2013;1:40-6.
5. Elbagermi MA, Edwards HGM, Alajtal AI. Monitoring of heavy metal content in fruits and vegetables collected from production and market sites in the misurata Area of Libya. Int Scholarly Res Network (ISRN) Anal Chem 2012:1-6.
6. Joint FAO/WHO Workshop on Fruit and Vegetables for Health (Kobe, Japan) Fruit and vegetables for health: Report of a Joint FAO/WHO Workshop, Kobe, Japan; 2004.
7. Ogunkunle ATJ, Bello OS, Ojofeitimi OS. Determination of heavy metal contamination of street-vended fruits and vegetables in Lagos state, Nigeria. Int Food Res J 2014;21:2115-20.
8. Hough RL, Breward N, Young SD. Assessing potential risk of heavy metal exposure from consumption of home-produced vegetables by Urban Populations. Environ Health Perspectives 2004;112:215-21.
9. Doherty VF, Sogbanmu TO, Kanife UC, Wright O. Heavy metals in vegetables collected from selected farm and market sites in Lagos, Nigeria, Global. Adv Res J Environ Sci Toxicol 2012;1:137-42.
10. Ramteke S, Sahu BL, Dahariya NS, Patel KS, Blazhev B, Matini L. Heavy metal contamination of vegetables. J Environ Protection 2016;7:996-1004.
11. Labhade KR. Assessment of heavy metal contamination in vegetables grown in and Around Nashik City, Maharashtra State, India. IOSR J Appl Chem 2013;5:9-14.
12. Tchounwou PB, Yedjou CG, Patlolla AK, Sutton DJ. Heavy metals toxicity and the environment. NIH Public Access 2012;101:133–64.
13. Gemede HF, Ratta N, Haki GD, Woldegiorgis AZ, Beyene F. Nutritional quality and health benefits of okra (Abelmoschus esculentus): a review. J Food Process Technol 2015;6:458.
14. [Last accessed on 29 Mar 2017]
15.,75.8489531,194m/data. [Last accessed on 01 Apr 2017]
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How to Cite
Yashwant, D. S., C. P. Mohan, and B. V. Adavirao. “GREY WATER FARMING OF LADIES FINGER AND CLUSTER BEANS-INDUCES DIETARY TOXICITY?”. International Journal of Current Pharmaceutical Research, Vol. 9, no. 4, July 2017, pp. 104-8, doi:10.22159/ijcpr.2017v9i4.20969.
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