ANTI-INFLAMMATORY ACTIVITY OF MANILKARA ZAPOTA LEAF EXTRACT


Kamalakararao Konuku, Krishna Chaithanya Karri, Velliyur Kanniappan Gopalakrishnan, Zenebe Hagos, Haftom Kebede, Tentu Kasi Naidu, Patricia Ponce Noyola, John Dogulas Palleti, Govinda Rao Duddukuri Rao Duddukuri

Abstract


Objective: Manilkara zapota is a medicinal plant which is native to Mexico and Central America, and widely distributed in India. Various parts of this plant are traditionally used for treatment of several diseases, including inflammation-associated ailments. The main aim of the present study is to evaluate the anti-inflammatory potential of ethyl acetate and methanolic extracts of M. zapota leaf.

Methods: In vitro secretary phospholipase A2 (PLA2) and 5-Lipoxygenase (5-LOX) assays and In vivo studies using carrageenan induced rat paw edema model were performed to assess the anti-inflammatory activity of M. zapota leaf extracts.

Results: In vitro studies suggest that M. zapota leaf extracts exhibited significant SPLA2 and 5-LOX inhibitory activities. In in vivo studies M. zapota leaf extracts showed dose dependent inhibition of carrageenan induced paw edema in rats. The anti-inflammatory activity of ethyl acetate leaf extract was superior to methanolic extract.

Conclusion: This study concluded that ethyl acetate leaf extract of M. zapotaexhibited significant anti-inflammatory activity and warranted further investigation to isolate and identify the components.

 


Keywords


Manilkara zapota, COX-2, Phospholipase A2, TNF-α, IL-1β,IL-6, IFN-γ

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About this article

Title

ANTI-INFLAMMATORY ACTIVITY OF MANILKARA ZAPOTA LEAF EXTRACT

Keywords

Manilkara zapota, COX-2, Phospholipase A2, TNF-α, IL-1β,IL-6, IFN-γ

DOI

10.22159/ijcpr.2017v9i4.20977

Date

14-07-2017

Additional Links

Manuscript Submission

Journal

International Journal of Current Pharmaceutical Research
Vol 9, Issue 4, 2017 Page: 130-134

Online ISSN

0975-7066

Statistics

80 Views | 41 Downloads

Authors & Affiliations

Kamalakararao Konuku
Department of Biochemistry, Aditya Degree and PG College, Kakinada, Andhra Pradesh, India

Krishna Chaithanya Karri
Department of Chemistry, College of Natural and Computational Sciences Aksum University, Axum, Ethiopia

Velliyur Kanniappan Gopalakrishnan
Department of Chemistry, College of Natural and Computational Sciences Aksum University, Axum, Ethiopia

Zenebe Hagos
Department of Chemistry, College of Natural and Computational Sciences Aksum University, Axum, Ethiopia

Haftom Kebede
Department of Biology, College of Natural and Computational Sciences Aksum University, Axum, Ethiopia

Tentu Kasi Naidu
Department of Medical Laboratory Sciences, School of Health Sciences, Dilla University, Dilla, Ethiopia

Patricia Ponce Noyola
Department of Life Sciences, University of Guanajuato, Mexico

John Dogulas Palleti
Department of Life Sciences, University of Guanajuato, Mexico

Govinda Rao Duddukuri Rao Duddukuri
Dept of Biochemistry and Molecular Biology, School of Biological Science, Central University of Kerala, India


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