STUDY ON IMMUNOSTIMULATORY PROPERTY OF FEW SEAWEEDS INJECTED INTRAPERITONEALLY


D. Radhika, A. Mohaideen

Abstract


Objective: Three type of seaweed was chosen for the study namely Gracilaria corticata, Ulva lactuca and Stocheospermum marginatum and was extracted with ethanol.

Methods: The fishes [Common carp (Cyprinus carpio) (weight 10±5g)] were divided into five groups. They were infected with A. hydrophila (1.6 × 104 CFU/fish). The infected three groups were injected with seaweed extract (0.20 mg) suspended in saline solution. One group was injected only saline control the other one group was injected with ciprofloxacin (0.20 mg) (standard) suspended in saline. The experiment was carried out for 28 d. Every seven days interval the fishes were injected with seaweed extract and blood parameters of RBC, WBC, HB, Ht, MCV, MCH, and MCHC were recorded.

Results: The MCHC value of Gracilaria corticata was recorded in 28th day (29.28±1.15) g/l. In Stocheospermum marginatum, the MCHC value was found in (27.19±1.62) g/l on 28th day. The Ulva lactuca had a MCHC value of (26.80±2) g/l on 28th day.

Conclusion: From this study, we can understand that the fish injected with seaweed extracts as good Immunostimulants properties. 

 


Keywords


Seaweed, Common carp, Bacterial infection, Aeromonas hydrophila Blood parameter, RBC, WBC,HB, Ht, MCV, MCH, MCHC

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References


Siddhanata SK, Ramavat, Kalpana Mody, Chauhan VD. The biomedical potential of marine algae. J Sea Res Utilization 1991;15:149-57.

Vairappan CS, Daitoh M, Suzuki M, Abe T, Masuda M. Antibacterial halogenated metabites from the Malysian Laurencia species. Phytochemicals 2001;58:291-7.

Naylor R, Burke M. Aquaculture and ocean resources: raising tigers of the sea. Ann Rev Environ Resources 2005;30:185-218.

Grisez L, Ollevier F. Vibrio (Listonella) anguillarum infection in marine fish larviculture. In: Lavens P, Jaspers E, Roelande I. editors. 91-Fish and crustacean larviculture symposium, European Aquaculture Society, Gent, Special Publication; 1995. p. 497.

Abdul Kader, Mydeen KP, Haniffa MA. Evaluation of Antibacterial activity of medicinal plants on fish pathogen Aeromonas hydrophila. J Res Biol 2011;1:1-5.

Hernández Serrano P. Responsible use of antibiotics in aquaculture. FAO Fisheries Technical; 2005. p. 109.

Newman DJ, Cragg GM, Snader KM. Natural products as a source of new drugs over the period 1981-2002. J Natl Prod 2003;66:1022-37.

Oh KB, Lee JH, Chung SC, Shin J, Shin HJ, Kim HK. Antimicrobial activities of the bromophenols from the red alga Odonthalia corymbifera and some synthetic derivatives. Bioorg Med Chem Lett 2008;18:104-8.

Yokoyama HO. Studies on the origin, development and seasonal variations in the blood cells of the perch, Percaflavescens. PhD Thesis, University of Wisconsin, Madison, Wisconsin; 1947. p. 144.

Christensen GM, Faindt JT, Poeschi BA. Cells and certain physiological properties of brook trout (Salvelinusfontinalis) blood. J Fish Biol 1978;12:51-60.

Kumar S, NP Sahu, AK Pal, D Choudhury, S Yengkokpam, SC Mukherjee. Fish Shellfish Immunol 2005;19:331-44.

Tagboto S, Townson S. Antiparasitic properties of medicinal plants and other naturally occurring products. Advances in Parasitology. Academic Press; 2001. p. 199–295.

Zahir AA, Rahuman AA, Kamaraj C, Bagavan A, Elango G, Sangaran A, et al. Laboratory determination of efficacy of indigenous plant extracts for parasites control. Parasitol Res 2009;105:453–61.

Zheng D, Han L, Huang XS, Yu SS, Liang XT. Natural products in clinical trials: antiparasitic, antiviral and neurological drugs. Yao Xue Xue Bao 2007;42:576–82.

Alghazeer R, Whida F, Abduelrhman E, Gammoudi F, Naili M. In vitro antibacterial activity of alkaloid extracts from green, red and brown macroalgae from the western coast of Libya. Afr J Biotechnol 2007;12:7086–91.

Al-Saif, SS A, Abdel-Raouf N, El-Wazanani HA, Aref IA. Antibacterial substances from marine algae isolated from Jeddah coast of Red Sea, Saudi Arabia. Saudi J Biol Sci 2014;21:57–64.

Mendes M, Pereira R, Sousa-Pinto I, Carvalho AP, Gomes AM. Antimicrobial activity and lipid profile of seaweed extracts from the North Portuguese Coast. Int Food Res J 2013; 20:3337–45.

Dubber D, Harder T. Extracts of ceramium rubrum, mastocarpus stellatus and laminaria digitata inhibit the growth of marine fish pathogenic bacteria at ecologically realistic concentrations. Aquaculture 2008;274:196–200.

Banaee M, Mirvagefei AR, Rafei GR, Amiri BM. Effect of sub-lethal diazinon concentrations on blood plasma biochemistry. Int J Environ Res 2008;2:189-98.

Radhika D, Mohaideen A. Effect of utilization of seaweeds as an immunostimulant in Aeromonas hydrophila Infected Cyprinus carpio. World J Pharm Pharm Sci 2016;5:851-7.




About this article

Title

STUDY ON IMMUNOSTIMULATORY PROPERTY OF FEW SEAWEEDS INJECTED INTRAPERITONEALLY

Keywords

Seaweed, Common carp, Bacterial infection, Aeromonas hydrophila Blood parameter, RBC, WBC,HB, Ht, MCV, MCH, MCHC

DOI

10.22159/ijcpr.2017v9i4.20978

Date

14-07-2017

Additional Links

Manuscript Submission

Journal

International Journal of Current Pharmaceutical Research
Vol 9, Issue 4, 2017 Page: 135-139

Online ISSN

0975-7066

Statistics

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Authors & Affiliations

D. Radhika
Dept of Zoology and Research Centre, V. O. Chidambaram College, Thoothukudi

A. Mohaideen
Dept of Zoology and Research Centre, V. O. Chidambaram College, Thoothukudi


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