STUDIES ON IN VITRO EVALUATION OF ANTIBACTERIAL ACTIVITIES OF EUCALYPTUS GLOBULUS LABILL LEAF
Keywords:Agar well diffusion, Antibacterial activity, Eucalyptus globulus leaf, n-Hexane, Methanol, Streptomycin
Objective: The objective of the present investigation was to evaluate the antibacterial potential of n-hexane and methanolic leaf extracts of Eucalyptus globulus Labill.
Methods: The antibacterial potential of the leaf extracts were tested against some human pathogenic bacteria causing several diseases such as Staphylococcus aureus, Vibrio cholerae, Pseudomonas aeruginosa, Shigella flexneri, Salmonella typhi, Klebsiella pneumoniae, Salmonella paratyphi, Bacillus subtilis, Micrococcus luteus, Salmonella typhimurium, Escherichia coli, Bacillus circulans, Streptococcus mitis, Enterococcus faecalis by using agar well diffusion method. The concentration of test plant extracts was 20 mg/ml. The inhibitory activity of the leaf extracts was compared with streptomycin as reference antibiotic (RA). The concentration of (RA) was 0.5 mg/ml.
Results: The result of the study revealed that n-hexane extract of Eucalyptus globulus Labill. Leaf was highly effective against Micrococcus luteus (19.66Â±0.94 mm) and least effective against Shigella flexneri (12.33Â±2.05 mm) whereas the methanolic extract had high inhibition effect against Vibrio cholerae (17.66Â±1.24 mm) and least against Pseudomonas aeruginosa (10 mm). The zone of inhibition of reference antibiotics ranged between 28.33Â±0.94 mm (Streptococcus mitis) to 21.66Â±3.09 mm (Escherichia coli).Conclusion: The leaf extract of E. globulus may be useful as an alternative antimicrobial agent in natural medicine for the treatment of numerous infectious diseases.
Cowan MM. Plant products as antimicrobial agents. Clin Microbiol Rev 1999;12:564-82.
Belaiche P. TraitÃ© de phytothÃ©rapie et d'aromathÃ©rapie. Tome I. Maloine SA. Editeur. Paris; 1979. p. 125-7.
Abu-Shanab B, Adwan G, Abu-Safiya D, Jarrar N, Adwan K. Antibacterial activities of some plant extracts utilized in popular medicine in palestine. Turk J Biol 2004;28:99-102.
Adwan G, Abu-Shanab B, Adwan K, Abu-Shanab F. Antibacterial effects of nutraceutical plants growing in palestine on pseudomonas aeruginosa. Turk J Biol 2006;30:239-42.
Al-Bayati FA. Synergistic antibacterial activity between Thymus vulgaris and Pimpinella anisum essential oils and methanol extracts. J Ethnopharmacol 2008;116:403-6.
Ody P. The Complete Medicinal Herbal. Dorling Kindersley. London; 1994. p. 61.
Musyimi DM, Ogur JA. Comparative assessment of the antifungal activity of extracts from Eucalyptus globulus and Eucalyptus citriodora. Res J Phytochem 2008;2:35â€“43.
Baratta MT, Dorman HJ, Deans SG, Figueiredo AC, Barroso JG, Ruperto G. Antimicrobial and antioxidant properties of some commercial essential oils. Flavour Fragrance J 1998;13:235â€“44.
Gray AM, Flatt PR. Antihyperglycemic actions of Eucalyptus globulus (Eucalyptus) are associated with pancreatic and extrapancreatic effects in mice. J Nutr 1998;128:2319â€“23.
Lee KG, Shibamoto T. Inhibition of malonaldehyde formation from blood plasma oxidation by aroma extracts and aroma components isolated from clove and eucalyptus. Food Chem Toxicol 2001;39:1199â€“204.
Mota VDS, Turrini RNT, Poveda VDB. Antimicrobial activity of Eucalyptus globulus oil, xylitol and papain: a pilot study. Rev Esc Enferm USP 2015;49:215-9.
Saxena HO, Brahmam M. The Flora of Orissa. Vol. I-IV. Orissa Forest Development Corporation. Bhubaneswar; 1994-1996.
Khatoon A, Jethi S, Nayak SK, Sahoo S, Mohapatra A, Satapathy KB. Evaluation of in vitro antibacterial and antioxidant activities of Melia azedarach L. bark. IOSR. J Pharm Biol Sci 2014;9:14-7.
Sahoo S, Nayak D, Kumar GS, Jayakumari S. Antimicrobial investigation of Ammania baccifra Linn. against some urinary and gastro-intestinal tract infection-causing pathogens. Indian Drugs 2012;49:44-8.
DamjanoviÄ‡-Vratnica B, Äakov T, Å ukoviÄ‡ D, DamjanoviÄ‡ J. Antimicrobial effect of essential oil isolated from Eucalyptus globulus Labill. from Montenegro. Czech J Food Sci 2011;29:277â€“84.
Ghalem BR, Mohamed B. Antibacterial activity of leaf essential oils of Eucalyptus globulus and Eucalyptus camaldulensis. Afr J Pharm Pharmacol 2008;2:211-5.
Takahashi T, Kokubo R, Sakaino M. Antimicrobial activities of eucalyptus leaf extracts and flavonoids from Eucalyptus maculate. Lett Appl Microbiol 2004;39:60â€“4.
Enciso-DÃaz OJ, MÃ©ndez-GutiÃ©rrez A, De JesÃºs LH, Sharma A, Villarreal ML, Taketa AC. Antibacterial activity of Bougainvillea glabra, Eucalyptus globulus, Gnaphalium attenuatum, and propolis collected in mexico. Pharmacol Pharm 2012;3:433-8.