• Jay Vyas Faculty of Science, Pacific Academy of Higher Education and Research University, Udaipur (Rajasthan) India 313001
  • Shafkat Rana P. G. Department of Botany, Shri Govind Guru Govt. College, Banswara (Rajasthan) India 327001


Objective: The objective of this present study were to the biogenic synthesis of selenium nanoparticles using Aloe vera extract and check it’s antioxidant potential by ABTS, DPPH and FRAP assays.

Methods: In this study we investigated the clove of Aloe vera, which is used for the synthesis of selenium nanoparticles were characterized by using UV-Visible (UV-VIS) spectrophotometer, Transmission electron microscopy (TEM), Fourier transform spectroscopy (FTIR) and Energy dispersive X-Ray spectroscopy (EDAX) and ABTS, DPPH and FRAP assays for checked it’s antioxidant potential.

Results: The present study was carried out to synthesis of Selenium nanoparticles using extract of Aloe vera. UV-Vis Spectra at 350 nm with Aloe vera extract and observed as hollow and spherical particles in size ranging 7-48 nm which is found more stable more than two months. EDAX analysis was carried out to check the presoak of Selenium in nanoparticles. Results of EDAX, confirmed its present. TEM and SEAD represented addition evidence of formation of nanoparticles whereas SEAD indicates the particles were crystalline in nature. FT-IR analysis was carried out to identify the possible bio molecules and Aloe vera extract-metal ions interaction responsible for formation and stabilization of selenium nanoparticles. FRAP, ABTS and DPPH assay results sequester that Selenium nanoparticles prepared using Aloe vera extract possess more activity than extract alone.

Conclusion: The bio molecules of Aloe veraextract acted as stabilizing as well as capping agent leading to the formation of Selenium nanoparticles. Selenite has been proven to have antioxidant activity and is being used as chemoprevention agent in cancer diagnosis but same time it is toxic also. Elemental Selenium i.e. Selenium nanoparticles are less toxic form of selenium.


Keywords: Biogenic synthesis, Selenium nanoparticles, Aloe vera, Antioxidant potential


1. P Gopinath, Sonit Kumar Gogoi, Pallab Sanpui, Anumita Paul, Arun Chatopadhyay, Siddharth Shankar Ghosh. Signaling gene cascade in silver nanoparticle induced apoptosis. Colloids Surf B 2010;77:240–5.
2. CB Murray, CR Kagan, MG Bawendi. Synthesis and characterization of monodisperse nanocrystals and close-packed nanocrystal assemblies. Annual Rev Mater Sci 2000;30:545-610.
3. P Ayyub, R Chandra, P Taneja, AK Sharma, R Pinto. Synthesis of nanocrystalline material by sputtering and laser ablation at low temperatures. Appl Phys 2001;73:67-73.
4. Kumar Suranjit Prasad, Hirnee Patel, Tirtha Patel, Khusbu Patel, Kaliaperumal Selvaraj. Biosynthesis of Se nanoparticles and its effect on UV-induced DNA damage. Colloids Surf B 2013;103:261–6.
5. Prathna TC, Lazar Mathew, N Chandrasekaran, Ashok M Raichur, Amitava Mukherjee. Biomimetic synthesis of nanoparticles: science, technology and applicability. Biomimetics-Learning from nature; 2010. p. 1-20.
6. Siavas Irarani. Green synthesis of metal nanoparticles using plants. Green Chem 2011;13:2638-50.
7. CH Ramamurthy, KS Sampath, P Arun kumar, M Suresh Kumar, V Sujatha, K Prem kumar, et al. Green synthesis and characterization of selenium nanoparticles and its augmented cytotoxicity with doxorubicin on cancer cells. Bioprocess Biosyst Eng 2013;36:1131–9.
8. Mojtaba Shakibaie, Ahmad Reza Shahverdi, Mohammad Ali Faramarzi, Gholam Reza Hassanzadeh, Hamid Reza Rahimi, Omid Sabzevari. Acute and subacute toxicity of novel biogenic selenium nanoparticles in mice. Pharm Biol 2012;51:58–63.
9. Parisa Jafari Fesharaki, Pardis Nazari, Mojtaba Shakibaie, Sassan Rezaie, Maryam Banoee, Mohammad Abdollahi, et al. Biosynthesis of selenium nanoparticles using Klebsiella pneumonia and their recovery by a simple sterilization process. Brazilian J Microbiol 2010;41:461-6.
10. Deepa B, Ganesan V. Biogenic synthesis and characterization of selenium nanoparticles using the flower of bougainvillea spectabilis willd. Int J Sci Res 2013;4:690-5.
11. Kumar Suranjit Prasad, Tapan Amin, Saloni Katuva, Madhu Kumari, Kaliaperumal Selvaraj. Synthesis of water soluble CdS nanoparticles and study of their DNA damage activity. Arabian J Chem 2014. Doi:10.1016/j.arabjc.2014.05.033.
12. Garima Sharma, Ashish Ranjan Sharma, Riju Bhavesh, Jongbong Park, Bilguun Ganbold, Ju-Suk Nam, et al. Biomolecule-mediated synthesis of selenium nanoparticles using dried vitis vinifera (Raisin) extract. Molecules 2014;19:2761-70.
13. Dejian Huang, Boxin Ou, Ronald L Prior. The chemistry behind antioxidant capacity assays. J Agric Food Chem 2005;53:1841–56.
14. NS Rajurkar, Kunda Gaikwad. Evaluation of phytochemicals, antioxidant activity and elemental content of adiantum capillus veneris leaves. J Chem Pharm Res 2012;4:365-74.
15. Baby Joseph, S Justin Raj. Pharmacognostic and phytochemical properties of Aloe vera linn-an overview. Int J Pharm Sci Rev Res 2010;4:106-10.
16. Marino B Arnao, Antonio Cano, Manuel Acosta. The hydrophilic and lipophilic contribution to total antioxidant activity. Food Chem 2001;73:239–44.
17. W Brand-Williams, ME Cuvelier, C Berset. Use of free radical method to evaluate antioxidant activity. Lebensm Wiss Technol 1995;28:25–30.
18. Iris FF Benzie, JJ Strain. The ferric reducing ability of plasma (FRAP) as a measure of ‘‘antioxidant power’’: the FRAP assay. Anal Biochem 1996;239:70–6.
19. Deendayal Mandal, Mark E Bolander, Debabrata Mukhopadhyay, Gobinda Sarkar, Priyabrata Mukherjee. The use of microorganisms for the formation of metal nanoparticles and their application. Appl Microbial Biotechnol 2006;69:485-92.
20. Kavita KS, Syed Baker, Rakshith D, Kavitha HU, Yashwantha Rao HC, Harini BP, et al. Plants as green source towards synthesis of nanoparticles. Int Res J Biol Sci 2013;2:66-76.
21. Maria I Gil, Francisco A, Tomas-Barberan FA, Hess-Pierce B, Kader AA. Antioxidant capacities, phenolic compounds, carotenoids, and vitamin C contents of nectarine, peach, and plum cultivars from California. J Agric Food Chem 2002;50:4976–82.
22. Nidhi Singh, Prasenjit Saha, Karthik Rajkumar, Jayanthi Abraham. Biosynthesis of silver and selenium nanoparticles by Bacillus sp. JAPSK2 and evaluation of antimicrobial activity. Pharm Lett 2014;6:175-81.
23. Shreya Medda, Amita Hajra, Uttiya Deym, Paulomi Bose, Naba Kumar Mondal. Biosynthesis of silver nanoparticles from aloe vera leaf extract and antifungal activity against Rhizopus sp. and Aspergillus sp. Appl Nanosci 2014;5:875–80.
24. Maryam Zahin, Farrukh Aqil, Iqbal Ahmad. The in vitro antioxidant activity and total phenolic content of four Indian medical plants. Int J Pharm Pharm Sci 2009;1:88-95.
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How to Cite
Vyas, J., and S. Rana. “ANTIOXIDANT ACTIVITY AND BIOGENIC SYNTHESIS OF SELENIUM NANOPARTICLES USING THE LEAF EXTRACT OF ALOE VERA”. International Journal of Current Pharmaceutical Research, Vol. 9, no. 4, July 2017, pp. 147-52, doi:10.22159/ijcpr.2017v9i4.20981.
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