PRELIMINARY PHYTOCHEMICAL SCREENING AND ANTI-INFLAMMATORY EFFECT OF THE AQUEOUS EXTRACT OF TABERNAEMONTANA DIVARICATA FLOWER IN WISTER RATS


Deepankar Garga, Trishna Das

Abstract


Objective: To determine the phytochemical analysis and anti-inflammatory effect of the aqueous extract of Tabernaemontanadivaricata flower in Wister Rats.

Methods: The aqueous extract of fresh flowers of the plant Tabernaemontanadivaricata (family-Apocyanaceae) were prepared and analyzed for phytochemical constituents using standard methods. The anti-inflammatory activity of the plant extracts was examined with the help of rat models by taking diclofenac as a standard drug against T. divericata oil extract by using carrageenan induced rat paw edema method with the help of the instrument plethesmometer.

Results: The present experiment shows the phytochemical analysis, anti-inflammatory activity of the aqueous extract of fresh flowers of the plant Tabernaemontanadivaricata (family-Apocyanaceae). Various phytochemical analysis revealed the presence of saponins, flavonoids, glycosides, steroids, proteins, phenols, amino acids and tannins. The oil extract of the plant showed much effective anti-inflammatory activity against the standard drug.

Conclusion: It can be concluded aqueous extract of the flower of the plant Tabernaemontanadivaricata (family-Apocyanaceae) contain the high presence of phytochemicals. This extract was found to possess promising antimicrobial activity when compared with the standards.


Keywords


Tabernaemontanadivaricata, Phytochemicals, Rat paw edema, Anti-inflammatory

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References


Doe C, Bentley R, Behm DJ, Lafferty R, Stavenger R, Jung D, et al. Novel rho kinase inhibitors with anti-inflammatory and vasodilatory activities. J Pharmacol Exp Ther 2007;320:89-98.

Akerele O. Nature‘s medicinal bounty: don‘t throw it away. World Health Forum 1993;14:390–5.

Calixto JB. Efficacy, safety, quality control, marketing and regulatory guidelines for herbal medicines (phytotherapeutic agents). Braz J Med Biol Res 2000;33:179-89.

Menkin V. Biochemical mechanisms in inflammation. Br Med J 1960;1:1521.

Warrier PK, Nambiar VPK, Ramankutty C. Indian medicinal plants. Vol. II. Madras: Orient Longman Ltd; 1996. p. 232.

Pratchayasakul W, Pongchaidecha A, Chattipakorn N, Chattipakorn S. Ethnobotany and ethnopharmacology of Tabernaemontanadivaricata. Indian J Med Res 2008;127:317-35.

Evans WC. Trease and Evans. Pharmacognosy. 15th ed. Toronto: Harcourt Pub Ltd; 2002. p. 1-40.

Kandati. In vitro and in vivo anti-inflammatory activity of Andrographisserpyllifolia. Int Curr Pharm J 2012;1:199-204.

Khandelwal KR. Practical pharmacognosy techniques and experiments. India: Nirali Prakashan; 2010. p. 12-30, 32.

CK Kokate. Practical Pharmacognosy. Vallabh Prakashan. 4th edn. 14. Delhi; 2005. p. 178-82.

JB Harborne. Phytochemical methods. 3rd ed. London: Chapman and Hall; 1998.

Qamruzzamaa, Javed Akhtar Ansarib. Analgesic and anti-inflammatory effect of ethanolic extract of Tabernaemontanadivaricata L. flowers in rats. Scholar Res Library 2012;4:1518-22.

Arawwawala M, Thabrew I, Arambewela L, Handunnetti S. Anti-inflammatory activity of Trichosanthescucumerina Linn. in rats. J Ethnopharmacol 2010;131:538-43.

Ramachandran S, Rajinikanth B, Rajasekaran A, Manisenthil Kumar KT. Evaluation of anti-inflammatory and analgesic potential of methanol extract of Tectonagrandis flowers. Asian Pac J Trop Biomed 2011;1 Suppl:S155-S158.




About this article

Title

PRELIMINARY PHYTOCHEMICAL SCREENING AND ANTI-INFLAMMATORY EFFECT OF THE AQUEOUS EXTRACT OF TABERNAEMONTANA DIVARICATA FLOWER IN WISTER RATS

Keywords

Tabernaemontanadivaricata, Phytochemicals, Rat paw edema, Anti-inflammatory

DOI

10.22159/ijcpr.2017v9i5.22128

Date

21-09-2017

Additional Links

Manuscript Submission

Journal

International Journal of Current Pharmaceutical Research
Vol 9, Issue 5 (Aug-Sep), 2017 Page: 9-12

Online ISSN

0975-7066

Statistics

46 Views | 19 Downloads

Authors & Affiliations

Deepankar Garga
Girijananda Chowdhury Institute of Pharmaceutical Science, Azara, Guwahati 781017
India

Trishna Das
Girijananda Chowdhury Institute of Pharmaceutical Science, Azara, Guwahati 781017
India


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