DEVELOPMENT AND VALIDATION OF UV SPECTROSCOPIC METHOD FOR ESTIMATION OF LAMIVUDINE IN TABLET DOSAGE FORM


Kalpesh V. Sonar, Prabodh Sapkale, Anil Jadhav, Tushar Deshmukh, Swapnil Patil, Pallavi Murkute

Abstract


Objective: To develop and validate simple, rapid, linear, accurate, precise and economical UV Spectroscopic method for estimation of Lamivudine in tablet dosage form.

Methods: The drug is freely soluble in analytical grade water. The drug was identified in terms of solubility studies and on the basis of melting point done on melting point apparatus of Equiptronics. It showed absorption maxima were determined in analytical grade water. The drug obeyed the Beer’s law and showed a good correlation of concentration with absorption which reflects in linearity. The UV spectroscopic method was developed for estimation of lamivudine in tablet dosage form and also validated as per ICH guidelines.

Results: The drug is freely soluble in analytical grade water, slightly soluble in methanol and practically insoluble in acetone. So, the analytical grade water is used as a diluent in the method. The melting point of lamivudine was found to be 160-161˚C (uncorrected). It showed absorption maxima 268 nm in analytical grade water. On the basis of the absorption spectrum, the working concentration was set on 10µg/ml (PPM). The linearity was observed between 6-14 μg/ml (PPM). The results of the analysis were validated by recovery studies. The recovery was found to be 98.7, 101 and 99.2% for three levels respectively. The % RSD for precision was found to be 0.62%.

Conclusion: A simple, rapid, linear, accurate, precise and economical UV Spectroscopic method has been developed for estimation of Lamivudine in tablet dosage form. The method could be considered for the determination of Lamivudine in quality control laboratories.


Keywords


Lamivudine, UV Spectrophotometer, Melting Point, Assay Method, Validation, Accuracy, Linearity, Ruggedness, Precision

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About this article

Title

DEVELOPMENT AND VALIDATION OF UV SPECTROSCOPIC METHOD FOR ESTIMATION OF LAMIVUDINE IN TABLET DOSAGE FORM

Keywords

Lamivudine, UV Spectrophotometer, Melting Point, Assay Method, Validation, Accuracy, Linearity, Ruggedness, Precision

DOI

10.22159/ijcpr.2017v9i6.23658

Date

14-11-2017

Additional Links

Manuscript Submission

Journal

International Journal of Current Pharmaceutical Research
Vol 9, Issue 6 (Oct-Nov), 2017 Page: 86-89

Online ISSN

0975-7066

Statistics

14 Views | 6 Downloads

Authors & Affiliations

Kalpesh V. Sonar
Department of Pharmaceutical Chemistry, Arunamai College of Pharmacy, North Maharashtra University, Mamurabad, Jalgaon (MH), India 425001

Prabodh Sapkale
Department of Pharmaceutical Chemistry, Arunamai College of Pharmacy, North Maharashtra University, Mamurabad, Jalgaon (MH), India 425001

Anil Jadhav
Department of Pharmaceutical Chemistry, Sandip Institute of Pharmaceutical Sciences, Pune University, Nasik (MH) India 422231

Tushar Deshmukh
Department of Pharmaceutical Chemistry, Arunamai College of Pharmacy, North Maharashtra University, Mamurabad, Jalgaon (MH), India 425001

Swapnil Patil
Department of Pharmaceutical Chemistry, Arunamai College of Pharmacy, North Maharashtra University, Mamurabad, Jalgaon (MH), India 425001

Pallavi Murkute
Department of Pharmaceutical Chemistry, Arunamai College of Pharmacy, North Maharashtra University, Mamurabad, Jalgaon (MH), India 425001


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