PHYTOCHEMICAL SCREENING AND RENAL EFFECTS OF ETHANOLIC EXTRACT OF ERIOBOTRYA JAPONICA FRUITS AND SEEDS IN ALLOXAN INDUCED DIABETIC RATS
Objective: The main aim of the present study was to evaluate the phytochemical constituents, and renal effects of the ethanolic extract of the fruits and seeds of Eriobotrya japonica, commonly called as Loquat in Kashmir. So far, no such study was carried in Kashmir.
Methods: Phytochemical screening involved the standard procedures. Diabetes was induced in albino rats using alloxan monohydrate (120 mg/kg b. w) given i. p prepared in sterile saline. Rats were divided into 8 groups of 6 rats in each group. The ethanolic extract of Eriobotrya japonica fruits were given in doses of 50 mg/kg, 100 mg/kg, and 200 mg/kg b. w. whereas the ethanolic extract of Eriobotrya japonica deeds were given in doses of 100 and 200 mg/kg b.w. The renal effects were determined by estimating the kidney function tests i.e., serum urea levels, serum creatinine levels and serum total proteins. The effects were compared with the normal control rats.
Results: Phytochemical screening revealed the presence of some useful phytoconstituents. alkaloids, glycosides and flavonoids in both extracts. The renal effects showed changes in alloxan-induced diabetic rats when treated with the ethanolic extract of the fruit and seeds of Eriobotrya japonica.
Conclusion: The ethanolic extract of E. J units and seeds have some useful Phytonstihut and there are some renal changes by the ethanolic extraction of E. B. F and S when admi to alloxaun inderced diabetic rats.Â
2. King H. Diabetes mellitus: a growing international health care problem. Int Diab Monitor 1997;9:1-6.
3. Grover JK, Yadav S, Vats. Medicinal plants of India with anti-diabetic potential. J Ethnopharmacol 2002;81:81-100.
4. Rafiullah MRM, Siddiqui AW, Mir SR, Ali M, Pillai KK, Singh S. Antidiabetic activity of some Indian medicinal plants. Pharm Biol 2006;44:2.
5. Rastogi RP, Mehrotra BN. Compendium of Indian Medicinal Plants. Vol. V. New Delhi, India Publications and Information Directorate (CSIR); 1995. p. 405.
6. Rastogi RP, Mehrotra BN. Compendium of Indian Medicinal Plants. Vol. I. New Delhi, India, Publications and Informationâ€™s Directorate (CSIR); 1999. p. 326, 398.
7. Rastogi RP, Mehrotra BN. Compendium of India Medicinal Plants. Vol. II. New Delhi Publications and Information Directorate (CSIR); 1990. p. 398.
8. Rastogi RP, Mehrotra BN. Compendium of Indian Medicinal Plants. Vol. II. New Delhi Publications and Information Directorate (CSIR); 1991. p. 660.
9. Chopra RN, Nayar SL, Chopra IC. Glossary of Indian medicinal plants. CSIR Pub, New Delhi; 1966. p. 104.
10. Chopra RN, Chopra IC. A review of work on Indian medical plants, ICMR, New Delhi; 1955.
11. Baljinder S, Seena G, Dharmendra K, Vikas G, Bansal P. Pharmacological potentral of eriobotrya japonica-an overview. Int Res J Pharm 2010;1:95-9.
12. Chen J, Li WL, Wu JL, Ren BR, Zhang HQ. Hypoglycemic effects of a sesquiterpene glycoside isolated from leaves of loquat (Eriobotrya japonica (Thumb,) Lindl. Phytomedicine 2008;15:98-102.
13. El-Hossary GA, Fathy MM, Kassem HA. Phytochemical and biological investigations of Eriobotrya japonica growing in Egypt. Bull Faculty Pharm 2000;38:129-42.
14. Huang Y, Li J, Cao Q, Yu SC, Lv XW, Jin Y, et al. Anti-oxidative effect of triterpene acids of Eriobotrya japonica (Thumb). Lindl. Leaf in chronic bronchitis rats. Life Sci 2006;78:2749-57.
15. Ito H, Kobayashi E, Takamasto Y, Li SH, Hatano T, Sakagami H, et al. Polyphenols from Eriobotrya japonica and their cytotoxicity against human oral tumour cell lines. Chem Pharm Bull (Tokyo) 2000;48:687-93.
16. Yokoto J, Takuma D, Hamada A, Onogawa M, Yoshioka S, Kusenose M, et al. Scavenging of reactive oxygen species by Eriobotrya Japonica seed extract. Biol Pharm Bull 2006;29:467-71.
17. Harborne JB. Phytochemical methods, Chapman and Hall Ltd., London; 1973. p. 49-188.
18. Rafia Rasool, Bashir A Ganai, Seema Akbar, Azra Kamili. Phytochemical screening of Prunella vulgaris L. an important medicinal plant of kashmir pak. J Pharm Sci 2010;23:399-402.
19. Trease GE, Evans WC. Pharmacognosy. 11th edn. Brailliar Tiridel Can. Macmillian Publishers; 1989.
20. Varley H. Practical Clinical Biochemistry New Delhi CBS. Publishers and Distributors V edition; 1980. p. 1, 457.
21. Zender R, Jacot P. A kinetic method for analysis of creatinine using the DSA-50. Anal Lett 1972;5:143-52.
22. Lowry HD, Rosenbroguh NT, Farr AL, Randel RJ. Protein measurement with the folin phenol reagent. J Biol Chem 1951;193:265-75.
23. Trinder P. Determination of glucose in blood using glucose oxidase with an alternative oxygen acceptor. Ann Clin Biochem 1966;6:24-5.
24. Trinder P. Glucose oxidase method. Ann Clin Biochem 1969;6:20-4.
25. Michael JF. Diabetes treatment. Part 1. Diet and Exercise. Clin Diabetes 2007;25:105-9.
26. Pradeep R, Mohan V. The changing scenario of the diabetes epidemic: implications for India. Indian J Med Res 2002;116:121-32.