ANTIOXIDANT MEDIATED DEFENSE ROLE OF ECLIPTAALBA HERBAL EXTRACT AGAINST CCL4 INDUCEDTOXIC HEPATITIS
Objective: The present study focuses on the antioxidant mediated protective effect of a popular medicinal herbEcliptaalba against CCl4induced oxidative stress in the liver of rats.
Methods: Rats divided into four groups were administered with CCl4 andCCl4 along with methanol extract of leaves of Eclipta herb (200 and 400 mg/kg b. wt) for three weeks. At the end of treatment, rats were anaesthetized and blood samples were collected for serum separation. Biochemical analysis such as alanineaminotransferase (ALT), aspartateaminotransferase (AST), alkaline phosphatase (ALP), lactate dehydrogenase (LDH) and total bilirubin (TB) were done in serum. Liver tissue was used for glutathione (GSH), lipid peroxidation (LPO), gluatathione peroxidase (GPx), glutathione Stransferase (GST), superoxide dismutase (SOD)and catalase (CAT) analysis. CCl4 administration caused significant elevation of the serum enzymes and total bilirubin content.
Results: Antioxidant enzymes were drastically inhibited with significant reduction of glutathione and increased lipid peroxidation in CCl4 treated rats. Ecliptaalbaextracts administrated rats, however did not show much changes in marker enzyme level. Antioxidant enzyme status was also not affected and found nearer to the control levels. Increased gluatathione level and reduced lipid peroxidation was also evident in Eclipta alba treated rats.
Conclusion: The findings show that Eclipta alba extract offers better protection against the free radical toxicity of CCl4.
2. Priya G, Chellaram C. In vivo hepatoprotective effect of Trianthemadecandra extracts on carbon tetrachloride induced Rats. J Chem Pharm Res 2011;3:154-8.
3. Bhargava, Seshadri TR. Chemistry of medicinalplants: Eclipta alba and Wedeliacalendulacea. J Res Indian Med 1974;9:9-15.
4. Cabre, Camps. Time courseof changes in lipid peroxidation and glutathione metabolism in rats withcarbon tetrachloride-induced cirrhosis. Clin Exp Pharmacol Physiol 2000;27:694-9.
5. Cassidy WM, Reynolds TB. Serum lactic dehydrogenase in the differential diagnosis of acute hepatocellular injury. J Clin Gasteroenterol 1994;19:118-21.
6. Ellman GL. Tissue sulfhydryl groups. Arch Biochem Biophys 1959;82:70-7.
7. Ferenci P, Dragosics B. Randomized controlled trial of silymarin treatment in patients with cirrhosis of the liver. J Hepatol 1989;9:105-13.
8. Habig WH, Pabst MJ, Jakoby WB. Glutathione-stransferases: the first enzymatic step in mercapturic acid formation. J Biol Chem 1974;249:7130â€“9.
9. Hagerstrand I. Distribution of alkaline phosphatase activity inhealthy and diseased human liver tissue. Acta Path Microbiol Scand A 1975;83:519-26.
10. Handa SS, Sharma A, Chakravarti KK. Natural products and plants as liver protective drugs. Fitotherapia 1986;57:307-12.
11. Hensley K, Robinson K, Gabbita SP, Salsman S, Floyd RA. Reactive oxygen species, cell signalling, and cell injury. Free Radical Biol Med 2000;28:1456-62.
12. Higuchi H, Gores GJ. Mechanisms of liver injury: an overview. Curr Mol Med 2003;3:483-90.
13. Kaplowitz N. Biochemical and cellular mechanisms of toxic liver injury. Semin Liver Dis 2002;22:137-44.
14. Kaplowitz, Ookhtens M. The regulation of hepatic glutathione. Annu Rev Pharmacol Pharmacol Toxicol 1985;25:715-44.
15. Kataki MS, Ahmad AZ, Avasthi D, Tomar B, Mehra P, Yadav RS, et al. In vitro antioxidant profile of Wedeliacalendulacea leaves. Pharmacologia 2012;3:75-83.
16. King EJ, Coxon RV. Determination of bilirubin with precipitation of the plasma proteins. J Clin Path 1950;3:248-59.
17. King PD, Perry MC. Hepatotoxicity of chemotherapy. The Oncologist 2001;6:162-76.
18. Loguercio C, Federico A. Oxidative stress in viral and alcoholic hepatitis. Free Radical Biol Med 2003;34:1-10.
19. McClements J, Decker EA. Lipid oxidation in oil-water emulsions: impact of molecular environment on chemical reactions in the heterogeneous food system. J Food Sci 2000;65:1270â€“82.
20. Meena AK, Rao MM, Meena RP, Panda P, Renu. Pharmacological and phytochemical evidence for the plants Wedeliagenusâ€“a review. Asian J Pharm Res 2011;1:7-12.
21. Mehendale HM. Role of hepatocellular regeneration and hepatocellular healing in the final outcome of liver injury: a two stage model of toxicity. Biochem Pharmacol 1991;42:1115-62.
22. Misra H, Fridovich I. The role of superoxide anion in the autooxidation of epinephrine and a simple assay for superoxide dismutase. J Biol Chem 1972;247:3170-5.
23. Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxidation in animal tissue by the thiobarbituric acid reaction. Anal Biochem 1979;95:351-8.
24. Rao KS, Recknagel RO. Early onset of lipoperoxidation inrat liver after carbon tetrachloride administration. Exp Mol Pathol 1968;9:271-8.
25. Recknagel RO, Glende EA Jr. Carbon tetrachloride hepatotoxicity: an example of lethal cleavage. CRC Crit Rev Toxic 1973:2:263-97.
26. Reitman S, Frankel S. A colourimetric method for the determination of serum glutamic oxaloacetic and glutamic pyruvic transaminases. Am J Clin Pathol 1957;28:56-63.
27. Rotruck JT, Pope AL, Gauther HE, Swanson AB, Hafeman DG, Hoekstna WG. Selenium: biochemical roles as a component of glutathione peroxidase. Science 1973;179:588-90.
28. Subrata De, Ravishankar B, Bhavsar GC. Plants with the hepatoprotective activity-a review. Indian Drugs 1994;30:355-63.
29. Takahara S, Hamilton BH, Nell JV, Ogubra TY, Nishimura ET. Hypocatalasemia: a new genetic carrier state. J Clin Invest 1960; 39:610â€“9.
30. Wagner H, Geyer B, Kiso Y, Hikino H, Rao GS. Coumestans as the main active principles of the liver drugs Eclipta alba and Wedelia calendulacea. Planta Med 1986;5:370-4.