PHYTOCHEMICAL ANALYSIS AND ANTIBACTERIAL ACTIVITY OF IPOMOEA INDICA PLANT LEAF EXTRACTS
Keywords:Ipomoea indica, Phytochemical screening, Antibacterial activity, Disc diffusion
Objective: The objective of this study was to phytochemical analysis and antibacterial activity of the Ipomoea indica plant leaves extracts.
Methods: The crude powder of the plant leaf samples of I. indica was extracted using methanol and aqueous solvents and subjected to qualitative phytochemical analysis. The antibacterial activity of the aqueous and methanol extracts was tested at different for concentrations. The extract was tested against bacterial strains like Staphylococcus aureus, Enterococcus faecalis, Salmonella typhi, and Escherichia coli.
Results: The phytochemical screening revealed the presence of alkaloids, flavonoids, terpenoids, glycoside, reducing sugars, saponins, steroids and tannins in the solvent extract. The antibacterial activity was performed by using the disc diffusion method, where the largest zone of inhibition was obtained with aqueous extract 75Âµ concentration against Salmonella typhi (26 mm). The antibacterial activity was compared with the standard antibiotics like Streptomycin.
Conclusion: This study suggests that the aqueous extract of the I. indica leaves possesses bioactive compounds with antibacterial activity against the bacterial strains, which can be used to formulate new antibacterial drugs for disease control.
Kumari SS, Rao SVS, Misra S, Murty US. Antifungal activity of Turbinaria conoides and evaluation for the effective concentration against the infection of Beauveria bassiana in silkworm larvae. Res J Microbiol 2011;6:115-23.
Rao GB, Umamaheshwara Rao P, Sambasiva Rao E, Mallikarjuna Rao T, Pranitha VS. Evaluation of in vitro antibacterial activity and anti-inflammatory activity for different extracts of Rauwolfia tetraphylla L. root bark. Asian Pacific J Trop Biomed 2012;2:818â€’21.
Padmaa MP, Leena JP, Angelin ST. Genius salacia: a comprehensive review. J Nat Remed 2010;8:116-31.
Vander Waaij D, Nord CE. The fluoroquinolone antibacterials: past, present and future perspectives. Int J Antimicrob Agents 2000;16:191-7.
Krishnaiah D, Devi T, Bono A, Sarbatly R. Studies on phytochemical constituents of six Malaysian medicinal plants. J Med Plants Res 2009;3:67-72.
Krishnaiah D, Sarbatly R, Bono A. Phytochemical antioxidants for health and medicineâ€“a move towards nature. Biotechnol Mol Biol Rev 2007;1:97-104.
Edeoga HO, Okwu DE, Mbaebie BO. Phytochemical constituents of some Nigerian medicinal plants. Afr J Biotech 2005;4:685-8.
Okunade AL. Review ageratum conyzoides L. (Asteraceae). Fitoterapia. 2002;73:1-16
Porwal Omji, Saxena Rubha, Nanjan Moola Joghee. Antioxidant activity of Ipomoea leari. J Drug Delivery Ther 2012;2:79-85.