PHYTOCHEMICAL ANALYSIS OF SOME SELECTED VARIETIES OF PIPER BETLE L.
Keywords:GC-MS, Eugenol, Hybrid variety 1, Hybrid variety 2, Jaipur Bangla, Karpoori, Local variety, Sirugamani
Objective: To Analyse the major phytochemical components in selected varieties of P. betle leaves using GC-MS.
Methods: P. betle leaves were shade dried and pulverized to powder in a mechanical grinder. The powder was successively extracted with ethanol (40-60 Â°C). The extracts were concentrated under reduced pressure in a rotary evaporator. The ethanolic extracts of the plant leaves were used for GC-MS analysis.
Results: In the present study ten chemicals were identified and compared from the P. betle varieties, the major component being Eugenol, which is responsible for the flavour and aroma of the leaves.
Conclusion: Variety Karpoori possesses the highest content of Eugenol and could be used as a promising variety in the pharmaceutical industry. The components of essential oil can also serve as a determinant to distinguish different varieties of betle vine cultivars.
Gamble JB. Flora of the presidency of madras II. Botanical Survey of India, Howrah, India; 1957.
Satish AB, DR Verma, RV Gavankar, C Nikhil, V Didwana, A Trikannad. Phytochemistry, pharmacological profile and therapeutic uses of Piper betle linn.-an overview. Res Rev: J Pharmacogn Phytochem 2013;1:10-9.
Bajpai V, D Sharma, B Kumar, KP Madhusudanan. Profiling of Piper betle Linn. cultivars by direct analysis in real time mass spectrometric technique. Biomed Chromatogr 2010;24:1283-6.
Bissa S, D Songara, A Bohra. Traditions in oral hygiene: chewing of betle (Piper betle L.) leaves. Curr Sci 2007;92:26-8.
Kanjwani DG, TP Marathe, SV Chiplunkar, SS Sathaye. Evaluation of immunomodulatory activity of methanolic extract of Piper betle. Scandinavian J Immunol 2008;67:589-93.
Sugumaran M, M Poornima, S Venkatraman, M Lakshmi, S. Sethuvani. Chemical composition and antimicrobial activity of sirugamani variety of Piper betle linn leaf oil. J Pharm Res 2011;4:3424-6.
Kumar N, P Mishra, A Dube, S Battacharya, K Dikshit, S Ranade. Piper betle Linn.-A maligned pan asiatic plant with an array of pharmacological activities and prospects for drug discovery. Curr Sci 2010;99:922-32.
Palaniappan G, A Sengottiyan, T Saravanan. Betle leaf: the green gold of India: facts for you. EFY enterprises PVT Ltd., New Delhi. India; 2012. p. 21â€“4.
Gupta PC, CS Ray. Epidemiology of betle quid usage. Ann Acad Med Singapore 2004;33:31-6.
Williams SA. Betle quid chewing: a community perspective. In: Betle-Quid Chewing Among Bangladeshi Community in the United Kingdom, London. R Bedi, P Jones. Eds. Centre for Transcultural Oral Health; 1995. p. 11-25.
Gopalakrishnan S, E Vadivel. GC-MS analysis of some bioactive constituents of Mussaenda frondosa Linn. Int J Pharma Bosci 2011;2:313-20.
Elizabeth Thomas A, T Aneesh, Della Grace Thomas,
R Anandan. GC-MS analysis of phytochemical compounds present in the rhizomes of Nervilia aragoana gaud. Asian J Pharm Clin Res 2013;6:68-74.
Chandra Vikash, Tripathi Shalini, NK Verma, DP Singh, SK Chaudhary, Roshan Asha. Piper Betle: phytochemistry traditional use and pharmacological activity-a review. IJPRD 2012;4:216-23.
Santhanam G, S Nagarajan. Wound healing activity of Curcuma aromatica and Piper betle. Fitoterapia 1990;61:458-9.
Rathee JS, BS Patro, S Mula, S Gamre, S Chattopadhyay. Antioxidant activity of Piper betle leaf extract and its constituents. J Agric Food Chem 2006;54:9046-54.
Mazura MP, N Hashim, RM Ali, Kiong, L Sui. Evaluation of Piper betle on platelet activating factor (PAF) receptor binding activities. Malaysian J Sci 2007;26:79-83.
Nalina T, ZHA Rahim. The crude aqueous extract of Piper betle L. and its antibacterial effect towards Streptococcus mutans. Am J Biotechnol Biochem 2007;3:10-5.
Pradhan D, KA Suri, DK Pradhan, P Biswasroy. Golden heart of nature: Piper betle L. Ixoreal biomed. Pvt. Ltd., APIIC, Green Industrial Park, Jadcherla, Mahaboobnagar, Hyderabad, Andhra Pradesh. India. J Pharmacogn Phytochem 2013;1:6.
Rawat AKS, RD Tripathi, AJ Khan, VR Balasubrahmanyam. Essential oil components as markers for identification of Piper betle L. cultivars. Biochem Systematic Ecol 1989;17:35-8.