ISOLATION AND IDENTIFICATION OF A FLAVONE FROM FRUIT PULP OF FERONIA LIMONIA


Jayashree V Hanchinalmath, Ramesh Londonkar

Abstract


Phenolic constituents are the principle bioactivity compounds exist in Feronia limonia; not much of the phenolic compounds are reported from the plant previously. The present paper describes the isolation, identification of flavonoid from medicinal plant Feronia limonia. Fruit pulp was defatted using petroleum ether and further extracted with methanol by Soxhlet extraction. Shinoda test and ferric chloride tests were carried out for preliminary confirmation of flavonoids; thin layer chromatography was employed for the separation of flavonoids. Isolated flavonoid was purified using silica gel column chromatography and further subjected to spectral characterization using FT-IR, 1H-NMR and LC-MS studies. Pure compound was identified as luteolin having molecular formula 2-(3,4-dihydroxyphenyl)-5,7-dihydroxy-4H-chromen-4-one, chemical formula C15H10O7 and molecular mass 283.35 by comparing their retention time (RT) and MS spectrum values with those that had been identified and the published data. With the help of the obtained phytochemical and spectral data, structure of the flavonoid was drawn using the software Chem Draw ultra version 12. Moreover, the compound 2-(3,4-dihydroxyphenyl)-5,7-dihydroxy-4H-chromen-4-one has been isolated for the first time from the plant Feronia limonia.

Keywords: Flavonoid, Luteolin, Fruit pulp, Feronia limonia, Methanolic extract.


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About this article

Title

ISOLATION AND IDENTIFICATION OF A FLAVONE FROM FRUIT PULP OF FERONIA LIMONIA

Date

07-10-2014

Additional Links

Manuscript Submission

Journal

International Journal of Current Pharmaceutical Research
Vol 6, Issue 4 (Oct-Dec 2014) Page: 28-31

Online ISSN

0975-7066

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230 Views | 370 Downloads

Authors & Affiliations

Jayashree V Hanchinalmath

Ramesh Londonkar


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