BIOCHEMICAL CHANGES OF VARIOUS METABOLITES IN CITRULLUS COLOCYNTHIS (LINN) SCHRAD
Citrullus colocynthis (Bitter apple) is a medicinally important plant. It is also known as colocynth. It belongs to family cucurbitaceae. In the present set of investigations, biochemical changes of various cellular metabolites/enzymes were observed from in vivo (leaf, stem, fruit and root) and in vitro (callus and differentiating callus) of Citrullus colocynthis. Total soluble sugar content was observed maximum in differentiating callus while minimum amount was observed in callus. Maximum amount of reducing sugar was observed in callus while minimum amount was observed in root sample. Starch was observed maximum in root while minimum amount was observed in callus. Î±-amylase activity was maximum in callus and minimum in root sample. More Î±-amylase activity could be correlated to less starch contents and vice-versa.
Keywords: Primary metabolites, Total soluble sugar, Reducing sugar, Starch, Î±- amylase, Citrullus colocynthis.
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