THE CURATIVE EFFECT OF METHYLSULFONYLMETHANE ON AN EXPERIMENTAL MODEL OF ULCERATIVE COLITIS IN RATS
Objective: Ulcerative colitis is a chronic inflammatory bowel disease of unknown etiology that affects the mucosa and submucosa of the colon and rectum. It is associated with oxidative stress, neutrophil infiltration and production of inflammatory mediators. In the present study, the anti-inflammatory and antioxidant effects of Methylsulfonylmethane (MSM) in treatment the acetic acid (A) induced model for UC in rats were examined.
Methods: Ulcerative colitis was induced by intra colonic administration of 2 ml of 4% acetic acid in adult male Wistar rats which were divided into 3 groups: normal control (N), acetic acid (A) control, and MSM (1000 mg/kg). Rats received treatment for six consecutive days. On day 7, the rats were sacrificed, the colon was removed, the body weight, colon weight/length ratio, tissue glutathione (GSH) concentration, and macroscopic evaluations, were performed.
Results: The colon weight/length ratio was decreased significantly (P<0.05). The glutathione (GSH) concentration was increased significantly (P<0.05). The macroscopic parameters were decreased, but it didn’t reduce significantly in the MSM treated groups compared to group A. Contrariwise the parameters of group A. The levels of body weight were increased, and colon weight/length ratio was decreased significantly (P<0.05). The glutathione (GSH) concentration was increased significantly (P<0.05). The macroscopic parameters were decreased significantly in the MSM treated group, compared to the acetic acid-treated group.
Conclusions: Methylsulfonylmethane is an effective, safety natural product with little side effects, has a good curative effect in a dose (1000 mg/kg) on experimental Ulcerative colitis induced by acetic acid.
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