• S. NAVEENTAJ Department of Pharmacy, Sri Padmavati Mahila Visvavidyalayam, [Women’s University], Tirupati 517502, Andhra Pradesh [A. P.], India
  • Y. INDIRA MUZIB Department of Pharmacy, Sri Padmavati Mahila Visvavidyalayam, [Women’s University], Tirupati 517502, Andhra Pradesh [A. P.], India




Cubosomes, Amphiphilic, Drug payloads, Anti-cancer


Cubosomes are novel biocompatible drug delivery system and have honeycombed (cavernous) structures whose diameter size range from 10–500 nm. They appear like dots, which are likely to be spherical structures. Each dot corresponds to the presence of a pore containing aqueous cubic phase in the lipid water system. Cubosomes posse’s great significance in the field of cosmeceuticals and Pharmaceuticals due to its unique features and become an attractive choice of vehicle for in vivo drug delivery due to their low cost, safety, efficacy and versatility for controlled release application and functionalization. Cubosomes have a very simple method of preparation; biodegradability of selected lipids has the capability to encapsulate hydrophobic and hydrophilic substances. Cubosomes are considered to be versatile systems, and prepared cubosomes can be administrated by different ways such as oral, percutaneous and parenteral routes. On the whole, cubosomes offer high consequence in nano-based drug preparations for melanoma (skin cancer) treatment, targeted drug delivery systems and comprise a wide range of applications in many areas and are characterized by various parameters. Consequently, cubosomes are in progress forward of awareness in the Pharmaceutical division. This review article mainly focuses on the methods of preparation, advantages, and applications of cubosomes.


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1. Andersson S, Jacob M, Ladin S, Larsson K. Structure of the cubosomes-a closed lipid bilayer aggregate. Zeitschrift Fur Kristallographie 1995;210:315-8.
2. Schwarz US, G Gompper. Bicontinous surfaces in self assembling amphiphilic systems. Lect Notes Phys 2002;600: 107-51.
3. Saurabh Bansal, Chandan Prasad Kashyap, Geeta Aggarwal. A comparative review on vesicular drug delivery system and stability issues. Int J Res Pharma Chem 2012;2:704-13.
4. Yosra SRE, Samar ME, Doaa AA, Ossama YA. Novel piperine-loaded tween-integrated monoolein cubosomes as brain-targeted oral nanomedicine in Alzheimer’s disease: pharmaceutical,biological and toxicological studies. Int J Nanomed 2015;10:5459-73.
5. Ruchi S, Gurvinder K, Deepak NK. Fluconazole loaded cubosomal vesicles for topical delivery. Int J Drug Dev Res 2015;7:32-41.
6. Sindhumol PG, Thomas M, Mohancharan PS. Phytosomes: a novel dosage form for enhancement of bioavailability of botanicals and nutraceuticals. Int J Pharm Pharm Sci 2010;4:10-4.
7. Schreier H, J Bouwstra. Liposomes and niosomes as topical drug carriers: dermal and transdermal delivery. J Controlled Release 1994;30:1-15.
8. Drummond CJ, Fong C. Surfactant self-assembly objects as novel drug delivery vehicles. Curr Opin Colloid Interface Sci 2000;4:449-56.s
9. Karami Z, Hamidi M. Cubosomes: remarkable drug delivery potential. Drug Discovery Today 2016;21:789–801.
10. Luzzati V, Tardieu A, Gulik Krzywicki T, Rivas E, Reiss Husson F. Structure of cubic phases of lipid-water systems. Nature 1968;220:485-8.
11. Fontell K, Mandell L, Ekwall P. Some isotropic mesophases in the systems containing amphiphilic compounds. Acta Chem Scanda 1968;22:3209-23.
12. Bouwstra JA, Honeywell-Nguyen PL. Vesicles as a tool for transdermal and drug delivery system Drug Discovery Today Drug Delivery Nanotech 2005;2:67-74.
13. Gustafsson J, Ljusberg Wahren H, Almgren M, Larsson K. Submicron particles of reversed lipid phases in water stabilized by a nonionic amphiphilic polymer. Langmuir 1997;13:6964-71.
14. Uchegbu FI, Vyas PS. Nonionic surfactant based vesicles in drug delivery. Int J Pharm 1998;7:33.
15. Jens Birk lauridsen. Food emulsifiers: surface activity, edibility manufacture, composition and application. J Am Oil Chem Soc 1976;53:400-7.
16. Larsson K. Cubic liquid-water phases: structures and biomembrane aspects. J Phys Chem 1989;93:7301-14.
17. Bhosale RR, Osmani RA, Harkare BR, Ghodake PP. Cubosomes. the inimitable nanoparticle drug carriers. Sch Acad J Pharm 2013;2:481-6.
18. Hyde ST, Andersson S, Ericsson B, Larsson K. A cubic structure consisting of a lipid bilayer forming an infinite periodic minimal surface of the gyroid type in the glycerol monooleate water system. Z Kristallogr 1984;168:213-9.
19. Clogston J, Craciun G, Hart DJ, Caffrey M. Controlling release from the lipidic cubic phase by selective alkylation. J Controlled Release 2005;102:441–61.
20. Nielsen L, Schubert L, Hansen J. Bioadhesive drug delivery systems. Characterisation of mucoadhesive properties of systems based on glyceryl mono-oleate and glyceryl monolinoleate. Eur J Pharm Sci 1998;6:231-9.
21. Wu H, Li J, Zhang Q, Yan X, Guo L, Gao X, et al. A novel small odorranalectin–bearing cubosomes: preparation, brain delivery and pharmacodynamic study on amyloid-b25-35-treated rats following intranasal administration. Eur J Pharma Bio Pharm 2012;80:368-78.
22. Almgren M, Edwards K, Gustafsson J. Cryotransmission electron microscopy of thin vitrified samples. Curr Opin Colloid Interface Sci 1996;1:270-8.
23. Lynch ML, Spicer PT. Cubic liquid crystalline composition and methods of preparation. USA Patent App; 2002.
24. Radiman SC Toprakcioglu, T Mcleish. Rheological study of ternary cubic phases. Langmuir 1994;10:61-7.
25. Spicer PT, Small WB, Lynch ML, Burns JL. Dry powder precursors of cubic liquid crystalline nanoparticles (cubosomes). J Nanopart Res 2002;4:297–311.
26. Patrick TS, Spicer L, William BS, Matthew LL, Janet LB. Dry powder precursors of Cubic liquid crystalline nanoparticles (cubosomes). J Nanopart Res 2002;4:297-311.
27. Gustafsson J, Ljusberg Wahren H, Almgren M, Larsson K. Cubic lipid water phase dispersed into submicron particles. Langmuir 1996;12:4611-13.
28. Spicer PT, Small WB, Lynch ML, Burns JL. Dry powder precursors of Soft cubic liquid crystalline nanoparticles (cubosomes). J Nanoparticles Res 2002;4:297-311.
29. MahaKa Khalifa. Miconazole nitrate based cubsosome hydrogels for topical application. Int J Drug Delivery 2015;7:1-12.
30. Cordero JA, Alarcon L, Escribano E, Obach R, Domenech J. A comparative study of the transdermal penetration of a series of non-steroidal anti-inflammatory drugs. J Pharm Sci 1997;86:503-8.
31. Muller RH, Petersen RD, Hommoss A, Pardeike J. Nanostructred lipid carriers (NLC) in cosmetic dermal products. Adv Drug Delivery Rev 2007;59:522-30.
32. Komal Tilekar, Prashant Khade, Sujit Kakade, Sachin Kotwal, Ravindra Patil. Cubosomes-a drug delivery system. IJPCBS 2014;4:812-24.
33. Geraghty PB, Attwood D, Collett JH, Sharma H, Dandiker Y. An investigation of the parameters influencing the bioadhesive properties of Myverol 18-99/water gels. Biomaterials 1997;18:63-7.
34. Murthy SK. Nanoparticles in modern medicine: state of the art and future challenges. Int J Nanomed 2007;2:129-41.
35. YR Hundekar, JK Saboji, SM Patil, BK Nanjwade. Preparation and evaluation of diclofenac sodium cubosomes for percutaneous administration. World J Pharm Pharm Sci 2014;3:523–39.
36. Gandhi A, Maji P. Self-emulsifying drug delivery system: an approach for enhancement of bioavailability of poorly water soluble drugs. Int J Pharm Integrated Life Sci 2013;1:1-16.
37. Anbarasan BA, Fatima Grace XA, Shanmuganathan SA. An overview of cubosomes. Smart drug delivery system. Sri Ramachandra J Med 2015;8:1-4.
38. Ljusberg Wahren H, Engstrom S, Gustafsson A. Bioadhesive properties of the monoolein-water system, Pharm Tech Eur 1995;11:14-7.
39. Yosra SRE, Samar ME, Doaa AA, Ossama YA. Novel piperine-loaded tween-integrated monoolein cubosomes as brain-targeted oral nanomedicine in Alzheimer’s disease: pharmaceutical, biological and toxicological studies. Int J Nanomed 2015;10:5459-73.
40. Deepak P, Dharmesh S. Cubosomes: a sustained drug delivery carrier. Asian J Res Pharm Sci 2011;1:59-62.
41. Bei D, Meng J, Youan BC. Engineering nanomedicines for improved melanoma therapy: progress and promises. Nanomedicine (Lond) 2010;5:1385-99.
42. Afriat I, Biatry B. Use of cubic gel particles as agents against pollutants, especially in a cosmetic composition. Eur Pat Appl (L'Oreal, Fr.). Ep; 2001.
43. Rizwan SB. Bicontinuous cubic liquid crystals as sustained delivery systems for peptides and proteins. Expert Opin Drug Delivery 2010;7:1133-44.
44. Omar SM, Ismail A, Hassanin KD, Dawoud SH. Formulation and evaluation of cubosomes as skin retentive system for topical delivery of clotrimazole. J Adv Pharm Res 2019;3:68-82.
45. Damani NC. Sustained release applications for treating periodontal disease. Eur Patent EP 0429224; 1991.
46. Engstrom S, Ljusberg Wahren H, Gustafsson A. Bioadhesive properties of the monoolein-water system. Pharm Tech Eur 1995;11:14-7.
47. Engstrom S, Lindman B, Larsson K. Method of preparing controlled release preparations for biologically active materials and resulting composition, US patent, 5151272; 1992.
48. Boyd BJ. Characterisation of drug release from cubosomes using the pressure ultrfiltration method. Int J Pharm 2003;260:239-47.
49. Engstorm S. Drug delivery from cubic and other lipid-water phases. Lipid Techonol 1990;2:42-5.
50. Sadhale Y, Shan JC. Glyceryl monooleate cubic phase gel as chemical stability enhancer of cefazolin and cefuroxime. Pharm Dev Technol 1998;3:549-56.



How to Cite

NAVEENTAJ, S., and Y. I. MUZIB. “A REVIEW ON LIQUID CRYSTALLINE NANOPARTICLES (CUBOSOMES): EMERGING NANOPARTICULATE DRUG CARRIER”. International Journal of Current Pharmaceutical Research, vol. 12, no. 1, Jan. 2020, pp. 5-9, doi:10.22159/ijcpr.2020v12i1.36820.



Review Article(s)