AMELIORATIVE ROLE OF SILYMARIN AGAINST ASPARTAME–INDUCED BIOCHEMICAL CHANGES, OXIDATIVE STRESS, INFLAMMATORY EFFECT AND GENOTOXICITY IN MALE ALBINO RATS

  • SABHA E. ELBALLAT Zoology Department, Faculty of Science, Zagazig University, Egypt
  • AL-SHIMAA M. ABAS Biochemistry Division, Chemistry Department, Faculty of Science, Zagazig University, Egypt

Abstract

Objective: Aspartame (ASP) is one of the most common artificial sweeteners. It has been recorded to be safe by World Health Organization. However, numerous publications have concluded that ASP is a genotoxic and carcinogenic sweetener.


Methods: The current study aims to examine the effect of ASP consumption (250 mg/kg body weight/day for 90 d) on some biochemical parameters, oxidative/antioxidative status in different tissues, Tumor Necrosis Factor-α (TNF-α), chromosomal aberration (CA) frequency and mitotic index (MI) percentage in addition to the possible ameliorative role of silymarin (50 mg/kg body weight/day for 90 d) against ASP-induced toxicity in male albino rats.


Results: The present results have confirmed that ASP is able to induce significant increase in the blood glucose level, liver, kidney and lipid function tests, Malondialdehyde (MDA) level, serum TNF-α level, frequency of CA and MI%. Meanwhile, Glutathione reduced level (GSH), Glutathione–S-transferase (GST) and catalase activity (CAT) were decreased by ASPadministration. Recovery group showed slight enhancement in all parameters but remained significant as compared to the control group. Co-administration of ASP with silymarin showed greater improvement than the recovery group.


Conclusion: Silymarin have an ameliorative role against biochemical oxidative stress, inflammatory changes in blood and different tissues, chromosomal aberrations and MI% induced by ASP administration.

Keywords: Aspartame, Silymarin, Inflammatory marker, Oxidative/anti-oxidative status, Chromosomal aberrations

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ELBALLAT, S. E., and A.-S. M. ABAS. “AMELIORATIVE ROLE OF SILYMARIN AGAINST ASPARTAME–INDUCED BIOCHEMICAL CHANGES, OXIDATIVE STRESS, INFLAMMATORY EFFECT AND GENOTOXICITY IN MALE ALBINO RATS”. International Journal of Current Pharmaceutical Research, Vol. 12, no. 1, Jan. 2020, pp. 41-55, doi:10.22159/ijcpr.2020v12i1.36831.
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