ANTIBACTERIAL ACTIVITY OF GOLDEN BERRY (PHYSALIS PERUVIANA) EXTRACT AGAINST ESCHERICHIA COLISPP. ISOLATES FROM MEATS IN ECUADOR

  • FAVIAN BAYAS-MOREJON Universidad Estatal de Bolívar, Departamento de Investigación y Vinculación, Centro de Investigación y Desarrollo Biotecnológico, Facultad de Ciencias Agropecuarias, Recursos Naturales y del Ambiente, 020150, Guaranda (Ecuador)
  • ANGELICA TIGRE-LEON Universidad Estatal de Bolívar, Departamento de Investigación y Vinculación, Centro de Investigación y Desarrollo Biotecnológico, Facultad de Ciencias Agropecuarias, Recursos Naturales y del Ambiente, 020150, Guaranda (Ecuador)
  • MARCELO TAPIA-VERDEZOTO Universidad Estatal de Bolívar, Departamento de Investigación y Vinculación, Centro de Investigación y Desarrollo Biotecnológico, Facultad de Ciencias de la Salud y del Ser Humano, 020150, Guaranda (Ecuador)
  • FABIAN FLORES-RIBADENEIRA Universidad Estatal de Bolívar, Departamento de Investigación y Vinculación, Centro de Investigación y Desarrollo Biotecnológico, Facultad de Ciencias de la Salud y del Ser Humano, 020150, Guaranda (Ecuador)

Abstract

Objective: The resistance of pathogenic bacteria to multiple antimicrobials has become a real global threat in terms of food safety and public health. To Study of antibacterial activity of golden berry extracts against E. coli isolated from meats in Ecuador.


Methods: Two solvents (water and ethyl alcohol) were used as well as three parts of the Goldenberry (mature berries, semi-mature berries and leaves) for its transformation into extracts. The extracts obtained were used in tests of anti-E. coli activity, contrasting with Ampicillin and gentamicin by means of the disk difusión method.


Results: Extracts in ethyl alcohol from semi-ripe berries and leaves were more effective at inhibiting the development of E. coli, with A2B1 and A2B3 treatments being those with the best antibacterial effect with 35.3% and 50% respectively. These results were close to those obtained with the antibiotics under study.


Conclusion: The use of uvilla extracts could be a viable natural alternative to act against pathogens.

Keywords: Antibacterial activity, Goldenberry extracts, E. coli, Meats

References

1. Huang K, Yang C, Huang S, Chen C, Lu Y, Lin Y. Recent advances in antimicrobial polymers: a mini-review. Int J Mol Sci 2016;17:1578.
2. ThuVu T, Kim H, Tran V, Le Dang K, Nguyen H, Kim H, et al. In vitro antibacterial activity of selected medicinal plants traditionally used in Vietnam against human pathogenic bacteria. Complementary Altern Med 2016;16:32.
3. Chavan MD, Bansode D. Studies on the effect of guava leaves extract against selected enteric bacteria. Asian J Pharm Technol Innovation 2015;3:97-102.
4. Kushwaha S, Betsy A, Chawla P. Effect of ashwagandha (Withaniasomnifera) root powder supplementation in the treatment of hypertension. EthnoMed 2012;6:111-5.
5. Mostafa A, Al-Askar A, Almaary K, Dawoud T, Sholkamy E, Bakri M. Antimicrobial activity of some plant extracts against bacterial strains causing food poisoning diseases. Saudi J Biol Sci 2018;25:361–6.
6. Çakir O, Pekmez M, Çepni E, Candar B, Fidan K. Evaluation of biological activities of physalisperuviana ethanol extracts and expression of Bcl-2 genes in HeLacells. Food Sci Technol (Campinas) 2014;34:422-30.
7. Corrales Bernal A, Vergara AI, Rojano B, Yahia E, Maldonado M. Características nutricionales y antioxidantes de la uchuva colombiana (Physalys peruviana L.) en tres estadios de su maduración, Arch Latinoam Nutr 2015;65:254-62.
8. Göztok F, Zengin F. The antimicrobial activity of physalis peruviana L. Bitlis Eren Univ J Sci Technol 2013;3:15-7.
9. Yen C, Chiu C, Chang F, Chen J, Hwang C, Hseu Y, et al. 4betaHydroxywithanolide E from Physalis peruviana (goldenberry) inhibits growth of human lung cancer cells through DNA damage, apoptosis and G2/M arrest. BMC Cancer 2010;10:46.
10. Cho S, Hiott L, Barrett J, McMillan E, House S, Humayoun S, et al. Prevalence and characterization of escherichia coli isolated from the upper oconeewatershed in Northeast Georgia. PLoS ONE 2018;13:e0197005. Doi:10.1371/journal.pone.0197005
11. Franco P, Ramirez L, Orozco M, Lopez L. Determination of escherichia coli and identification of the o157: h7 serotype in pork ?s meat commercialized in the most important supermarkets in cartagena, colombia. Revista Lasallista de Investigacion 2013;10:91-100.
12. Adzitey F, Saba C, Gabriel A. Antibiotic susceptibility of Escherichia coli isolated from milk and hands of milkers in Nyankpala community of Ghana. Curr Res Dairy Sci 2016;8:6-11.
13. Affonso Scaletsky I. Enteropathogenic Escherichia coli. Intech Open 2018;1-18. Doi.org/10.5772/intechopen.82861
14. Farahmandfar M, Moori Bakhtiari N, Gooraninezhad S, Zarei M. Comparison of two methods for detection of E. coli O157H7 in unpasteurized milk. Iran J Microbiol 2016;8:282–7.
15. Ponce A, Roura S, Del Valle CE, Moreira M. Antimicrobial and antioxidant activities of edible coatings enriched with natural plant extracts: in vitro and in vivo studies. Postharvest Biol Technol 2008;49:294-300.
16. Areiza N, Maldonado M, Rojano B. Extracto acuoso de uchuva (Physalis peruviana): actividades antiproliferativa, apoptotica y antioxidante. Perspectivas en Nutricion Humana 2013;15:41-5.
17. Shokeen P, Bala M, Tandon V. Evaluation of the activity of 16 medicinal plants against neisseria gonorrhoeae. Int J Antimicrob Agents 2009;33:86-91.
18. Devi WR, Singh S, Singh C. Antioxidant and anti-dermatophytic properties leaf and stem bark of Xylosma longifoliumclos. BMC Complement Altern Med 2013;13:155.
19. Settani L, Randazzo W, Palazzolo E, Moschetti M, Aleo A, Guarrasi V, et al. Sensorial variations of antimicrobial activity and chemical composition of essential oil sex tracted from three citrus limon L. Burm. cultivars. Nat Prod Res 2014;28:383-91.
20. Nair R, Kalariya K, Chanda S. Antibacterial activity of some selected Indian Medicinal Flora Turkish Journal. Int J Curr Microbiol Appl Sci 2015;6:1146-53.
21. Yilmaztekin M. Analys is of volatile components of cape gooseberry (Physalis peruviana L.) grown in Turkey by HSSPME and GC-MS. Sci World J 2014. https://doi.org/ 10.1155/2014/796097
22. Ramadan M, El-Ghorab A, Ghanem K. Volatile compounds, antioxidants, and anticancer activities of cape gooseberry fruit (Physalis peruviana L.): an in vitro study. J Arab Soc Med Res 2015;10:56-64.
Statistics
82 Views | 71 Downloads
Citatons
How to Cite
BAYAS-MOREJON, F., A. TIGRE-LEON, M. TAPIA-VERDEZOTO, and F. FLORES-RIBADENEIRA. “ANTIBACTERIAL ACTIVITY OF GOLDEN BERRY (PHYSALIS PERUVIANA) EXTRACT AGAINST ESCHERICHIA COLISPP. ISOLATES FROM MEATS IN ECUADOR”. International Journal of Current Pharmaceutical Research, Vol. 12, no. 2, Mar. 2020, pp. 115-8, doi:10.22159/ijcpr.2020v12i2.37506.
Section
Original Article(s)