PHARMACOLOGICAL STUDIES ON PLANTS BELONGING TO THE FAMILY LEGUMINOSAE
Objective: The study is an attempt to quantitatively measure the presence of Anti-Parkinsons drug Levodopa in members of the family Leguminosae.
Methods: Reverse phase HPLC, Phytochemical analysis carried out using Bioinformatic tools (ChemMine and Cytoscape). The dried seed samples of Mucuna pruriens (Linn.), Cassia fistula (Linn.), Lathyrus odoratus (Linn.), Glycine max (L. Merr.), and Mimosa pudica (L) were selected for the study of Levodopa, and compared with Tecoma stans from the family Bignoniaceae., and antimicrobial activity was conducted on selected bacteria.
Results: Mucuna pruriens showed the presence of Levodopa in quantifiable higher quantity i.e., 705.5985 ????g/20 ml, whereas other plant samples contained very less quantity. The antimicrobial activity of the selected plants on E. coli and Bacillus showed positive results.
Conclusion: The study proves the presence of Anti-Parkinsons drug Levodopa in Mucuna pruriens, whereas absent in the other plants. The selected plants prove the anti-bacterial activity on the selected bacteria.
2. G DeMaagd, A Philip. Parkinson’s disease and its management part 1: disease entity, risk factors, pathophysiology, clinical presentation, and diagnosis. P T 2015;40:504–32.
3. Parkinson’s Foundation. Available from: https://www.parkinson.org/Understanding-Parkinsons/Statistics. [Last accessed on 27 Feb 2020]
4. Overview | Parkinson’s disease in adults | Guidance | NICE. Available from: https://www.nice.org.uk/guidance/NG71. [Last accessed on 27 Feb 2020]
5. G Pezzoli, M Zini. Levodopa in Parkinson’s disease: From the past to the future. Expert Opinion Pharmacother 2010;11:627–35.
6. Levodopa (L-Dopa)-PubMed. Available from: https://pubmed. ncbi.nlm.nih.gov/29489269-levodopa-l-dopa/ [Last accessed on 28 Feb 2020].
7. M Furlanut, J Furlanut M, P Benetello. Erratum: monitoring of L-DOPA concentrations in Parkinson’s disease. Pharmacol Res 2001;43:423-7.
8. S Ovallath, B Sulthana. Levodopa: history and therapeutic applications. Ann Indian Acad Neurol 2017;20:185–9.
9. PubChem Update: Improved Access to Chemical Data-PubMed; 2019. Available from: https://pubmed.ncbi.nlm.nih.gov/ 30371825-pubchem-2019-update-improved-access-to-chemical-data/. [Last accessed on 28 Feb 2020]
10. PubChem Substance and Compound Databases-PubMed. Available from: https://pubmed.ncbi.nlm.nih.gov/26400175-pubchem-substance-and-compound-databases/. [Last accessed on 28 Feb 2020]
11. Y Djoumbou Feunang. ClassyFire: automated chemical classification with a comprehensive, computable taxonomy. J Cheminform 2016;8:1–20.
12. L Sathiyanarayanan, S Arulmozhi. Mucuna pruriens linn.-a comprehensive review. Pharmacogn Rev 2007;1:157-62.
13. R Katzenshlager. Mucuna pruriens in Parkinson’s disease: a double blind clinical and pharmacological study. J Neurol Neurosurg Psychiatry 2004;75:1672–7.
14. H Pulikkalpura, R Kurup, PJ Mathew, S Baby. Levodopa in mucuna pruriens and its degradation. Sci Rep 2015;5:1–9.
15. A Ali. Cassia fistula linn. a review of phytochemical and pharmacological studies. Int J Pharm Sci Res 2014;5:2125–30.
16. JF Kennedy, PMG Palva, MTS Corella, MSM Cavalcanti, LCBB Coelho. Lectins, versatile proteins of recognition: a review. Carbohydrate Polymers 1995;26:219–30.
17. AV Pawar, SJ Patil, SG Killedar. Uses of Cassia Fistula Linn as a medicinal plant. Pawar Aarti V Int J Adv Res Dev 2017;2:85–91.
18. Lathyrus odoratus (sweet pea). Available from: https://www. cabi.org/isc/datasheet/31584. [Last accessed on 28 Feb 2020]
19. HG Fried, S Narayanan, B Fallen. Characterization of a soybean (Glycine max L. Merr.) germplasm collection for root traits. PLoS One 2018;13:e0200463.
20. SR Mentreddy, AI Mohamed, N Joshee, AK Yadav. Edamame: a nutritious vegetable crop, ASHS Press; 2002.
21. RJ Hamilton. Lipid analysis in oils and fats. Springer US; 1997.
22. H Ahmad, S Sehgal, A Mishra, R Gupta. Mimosa pudica L. (Laajvanti): an overview. Pharmacogn Rev 2012;6:115–24.
23. R Rajendran, R Sundararajan. Preliminary phytochemical analysis and anti-bacterial activity of Mimosa pudica linn leaves. Int J Pharma Bio Sci 2010;5:53-5.
24. B Govindappa, M Sadananda, TS Channabasava, R Jeevitha, MK Pooja, KS Vinay. Antimicrobial, antioxidant activity and phytochemical screening of tecoma stans (L.) Juss. ex Kunth. J Phytol 2011;3:68-76.
25. S Verma. Phytochemical and pharmacological review study on Tecoma Stans Linn; 2016.
26. Online Shopping site in India: Shop Online for Mobiles, Books, Watches, Shoes and More-Amazon. In. Available From: https://www.amazon.in/ [Last accessed on 29 Feb 2020]
27. RN Vora, AN Joshi, NC Joshi. Comparison of l-dopa content in two varieties of broad beans (Vicia Faba) by different extraction techniques. World J Pharm Med Res 2017;3:271–4.
28. T Hasegawa, K Takahashi, T Fukiwake, M Saijo, Y Motoki. Enantiomeric determination of DOPA in dietary supplements containing mucuna pruriens by liquid chromatography/mass spectrometry. J Food Hyg Soc Japan 2013;54:379-83.
29. BN Singh, AKS Rawat, RM Bhagat, BR Singh. Black tea: phytochemicals, cancer chemoprevention, and clinical studies. Crit Rev Food Sci Nutr 2017;57:1394–410.
30. EE Bolton, Y Wang, PA Thiessen, SH Bryant. Chapter 12 PubChem: Integrated Platform of Small Molecules and Biological Activities Elsevier BV; 2008.
31. AK Virk, C Kumari, A Tripathi, A Kakade, X Li, S Kulshrestha. Development and efficacy analysis of a Moringa oleifera based potable water purification kit. J Water Process Eng 2018;27:37–46.
32. A Soumyanath, T Denne, A Hiller, S Ramachandran, L Shinto. Analysis of levodopa content in commercial mucuna pruriens products using high-performance liquid chromatography with fluorescence detection. J Altern Complement Med 2018;24:182-6.
33. H Haibin, S Jian, T Yechong. Determination of levodopa in mucuna macrocarpa wall by HPLC. Guangxi Zhiwu 2004;24:460-2.
34. AR Soares, R Marchiosi, R de Cassia Siqueira Soares, RB de Lima, WD dos Santos, O Ferrarese Filho. The role of L-DOPA in plants. Plant Signaling and Behavior 2014;9:e28275.
35. A Kulma, J Szopa. Catecholamines are active compounds in plants. Plant Sci 2007;172:433-40.
36. KB Ramya, S Thaakur. Herbs containing L-dopa: an update. Anc Sci Life 2007;27:50–5.
37. KM Shaw, AJ Lees, GM Stern. The impact of treatment with levodopa on parkinson’s disease. QJM An Int J Med 1980;49:283–93.
38. BV Manyam, M Dhanasekaran, TA Hare. Neuroprotective effects of the antiparkinson drug mucuna pruriens. Phyther Res 2004;18:706–12.
39. JB Calixto. Efficacy, safety, quality control, marketing and regulatory guidelines for herbal medicines (phytotherapeutic agents). Braz J Med Biol Res 2000;33:179–89.
40. SA Mastan, P Janaki Ramayya, L Mutyala Naidu, K Mallikarjuna. Antimicrobial activity of various extracts of Mucuna pruriens leaves. Biomed Pharmacol J 2009;2:55–60.
41. L Taylor. The healing power of rainforest herbs?: a guide to understanding and using herbal medicinals. Square One Publishers; 2005.
42. SL Jothy. Bioassay directed isolation of active compounds with antiyeast activity from a cassia fistula seed extract. Molecules 2011;16:7583–92.
43. V Duraipandiyan, S Ignacimuthu. Antibacterial and antifungal activity of cassia fistula L.: an ethnomedicinal plant. J Ethnopharmacol 2007;112:590–4.
44. S Mohamed. Anthocyanins and fatty acids from the flowers of lathyrus odoratus L. and their antimicrobial activity. Planta Med 2009;75:175.
45. SA Hosseini Chaleshtori, M Ataie Kachoie, SM Hashemi Jazi. Antibacterial effects of the methanolic extract of glycine max (Soybean). Microbiol Res (Pavia) 2017;8. Doi:10.4081/ mr.2017.7319
46. N Gandhiraja, S Sriram, V Meenaa, JK Srilakshmi, C Sasikumar, R Rajeswari. Phytochemical screening and antimicrobial activity of the plant extracts of mimosa pudica l. against selected microbes; 2009.
47. R Singh Tomar, V Shrivastava, S Kaushik. In vitro efficacy of methanolic extract of mimosa pudica against selected micro-organisms for its broad spectrum antimicrobial activity miomosa pudica-a broad spectrum antimicrobial herb view project biosynthesis of gold nanoparticles view project micro-organisms for its broad spectrum antimicrobial activity. Int J Curr Microbiol Appl Sci 2014;3:780-4.
48. HM Sbihi, S Mokbli, IA Nehdi, SI Al-Resayes. Physico-chemical properties of tecoma stans linn. seed oil: a new crop for vegetable oil. Nat Prod Res 2015;29:1249–55.
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