• AMALA DIVYA S. Department of Botany, Bharathiar University, Coimbatore, Tamil Nadu 641046
  • THAMARAIKANI V. Department of Botany, Bharathiar University, Coimbatore, Tamil Nadu 641046
  • SEKAR T. Department of Botany, Bharathiar University, Coimbatore, Tamil Nadu 641046



Sarcostemma brunonianum, Phytochemicals, Antioxidants, Anti-inflammatory


Objective: Sarcostemma brunonianum Wight and Arn is a potential medicinal plant belonging to Asclepiadaceae. Bioactive constituents of the plant support the application of treating various ailments in the traditional system of medicine. The study aims to determine the presence of various phytoconstituents in stem, root, and flowers.

Methods: Hot percolation method was carried out to obtain crude extracts using different solvent systems from low polar-high polar solvents ranging from petroleum ether, chloroform, (mid-polar) ethyl acetate, methanol and water. Estimation of total phenols, tannins and In vitro antioxidant, anti-inflammatory activities were evaluated for the determination of potential pharmaceutical drugs.

Results: The results revealed the presence of some phytoconstituents such as phenols, tannins, glycosides, gums and mucilages. Ascorbic acid, BSA, Rutin and Gallic acid were used as the reference standard. The total phenolic content was found to be high in stem methanol extract 440.84±69.99 mg/g Gallic acid equivalent, whereas the tannin content was 291.78±4.68 mg/g GAE. The result proves that the S. brunonianum stem methanol extract possesses antioxidant and anti-inflammatory activities when compared to reference standards. In vitro, Nitric oxide scavenging activity of stem showed a maximum % of inhibition in methanol stem extract (24.39µg/ml) and anti-inflammatory activity maximum inhibition was found to be (55.56 %) in stem methanol and flower(53.62 %). The IC50 (concentration required for 50% inhibition) was also calculated for the DPPH radical model.

Conclusion: This study results proclaims and justifies the role of folklore medicinal plant S. brunonianum in the treatment of inflammatory-related ailments and can be recommended for an effective drug.


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1. Sarvalingam A, Dhaarani V, Pavithra C, Sharmila S, Rajendran A. Inventory and ethnomedicinal plants used by rural people of Eastern Ghats of Tamil Nadu, India. J Ecobiotechnol 2017;9:5-12.
2. Amala Divya S, Thamarai Kani V, Vijaya Lakshmi. A report on some lactiferous species of maruthamalai hills of the Southern Western ghats of coimbatore district, India. Res Rev: J Ecol 2018;7:1–7.
3. Gritto MJ, Nandagopalan V, Doss A. Ethnobotanical study on the traditional healers in pachamalai hills of Eastern Ghats, Tamil Nadu, South India. J Med Plants Stud 2015;3:80-5.
4. Pandey S, Shukla A, Pandey S, Pandey A. Morphology, chemical composition and therapeutic potential of somlata (sarcostemma acidum wight. and arn.). Pharma Sci Monitor 2017;8:54-60.
5. Shailesh G, Seema K, Dwivedi S. In vitro anti-inflammatory activity of sarcostemma acidum wight and Arn. Indian herb by Human red blood cell membrane stabilization method. Int J 2011;2:184-8.
6. Abdel Kader MS, Hazazi AM, Elmakki OA, Alqasoumi SI. A survey on traditional plants used in Al Khobah village. Saudi Pharm J 2018;26:817-21.
7. Sekar K, Thangaraj S, Babu SS, Harisaranraj R, Suresh K. Phytochemical constituent and antioxidant activity of extract from the leaves of ocimum basilicum. J Phytol 2009.
8. Gangwar M, Gautam MK, Sharma AK, Tripathi YB, Goel RK, Nath G. Antioxidant capacity and radical scavenging effect of polyphenol-rich Mallotus philippenensis fruit extract on human erythrocytes: an in vitro study. Sci World J 2014. DOI:10.1155/2014/279451.
9. Tawaha K, Alali FQ, Gharaibeh M, Mohammad M, El-Elimat T. Antioxidant activity and total phenolic content of selected Jordanian plant species. Food Chem 2007;104:1372-8.
10. Melinda KP, Rathinam X, Marimuthu K, Diwakar A, Ramanathan S, Kathiresan S, et al. A comparative study on the antioxidant activity of methanolic leaf extracts of Ficus religiosa L, Chromolaena odorata (L.) king and rabinson, Cynodon dactylon (L.) pers. and tridax procumbens L. Asian Pacific J Trop Med 2010;3:348-50.
11. Tharun G, Pindi PK. Evaluation of antioxidant potential and antimicrobial activity of successive extracts of Pimpinella tirupatiensis. J Pharm Res 2013;7:817-22.
12. Evans WC. Trease and evans pharmacology. Harcourt Brace Company Asia. Pvt. Ltd. Singapore; 1997.
13. Herborne JB. A guide to modern techniques of plant analysis. Phytochemical Methods 1973. p. 5-11.
14. Odebiyi OO, Sofowora EA. Phytochemical screening of Nigerian medicinal plants II. Lloydia 1978;41:234.
15. Raaman N. Phytochemical techniques new India publishing agency. Chapter Six; 2006. p. 40-67.
16. Siddhuraju P, Manian S. The antioxidant activity and free radical-scavenging capacity of dietary phenolic extracts from horse gram (Macrotyloma uniflorum (Lam.) Verdc.) seeds. Food Chem 2007;105:950-8.
17. Siddhuraju P, Becker K. Studies on antioxidant activities of mucuna seed (Mucuna pruriens var utilis) extract and various non?protein amino/imino acids through in vitro models. J Sci Food Agric 2003;83:1517-24.
18. Prieto P, Pineda M, Aguilar M. Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E. Anal Biochem 1999;269:337-41.
19. Blois MS. Antioxidant determinations by the use of a stable free radical. Nature 1958;181:1199-200.
20. Rao MNA. Nitric oxide scavenging by curcuminoids. J Pharm Pharmacol 1997;49:105-7.
21. Mizushima Y, Kobayashi M. Interaction of anti?inflammatory drugs with serum proteins, especially with some biologically active proteins. J Pharm Pharmacol 1968;20:169-73.
22. Oyedapo OO, Akinpelu BA, Orefuwa SO. Anti-inflammatory effect of theobroma cacao, root extract. J Trop Med Plants 2004;5:161-6.
23. Roby MHH, Sarhan MA, Selim KAH, Khalel KI. Evaluation of antioxidant activity, total phenols and phenolic compounds in thyme (Thymus vulgaris L.), sage (Salvia officinalis L.), and marjoram (Origanum majorana L.) extracts. Industrial Crops Products 2013;43:827-31.
24. Hudaib M, Speroni E, Di Pietra AM, Cavrini V. GC/MS evaluation of thyme (Thymus vulgaris L.) oil composition and variations during the vegetative cycle. J Pharm Biomed Anal 2002;29:691-700.



How to Cite

S., A. D., T. V., and S. T. “ASSESSMENT OF PHYTOCHEMICALS AND IN VITRO ANTIOXIDANT, ANTI-INFLAMMATORY ACTIVITY OF SARCOSTEMMA BRUNONIANUM WIGHT AND ARN”. International Journal of Current Pharmaceutical Research, vol. 13, no. 1, Jan. 2021, pp. 64-69, doi:10.22159/ijcpr.2021v13i1.40819.



Original Article(s)