• MAYANGLAMBAM MEDHABATI Department of Pharmacology, Regional Institute of Medical Sciences, Imphal, India
  • LAISHRAM BABYCHA Department of Pharmacology, Jawaharlal Nehru Institute of Medical Sciences, Imphal, India
  • ABHISHEK BHATTACHARJEE Department of Pharmacology, Regional Institute of Medical Sciences, Imphal, India
  • NGANGOM GUNINDRO Department of Pharmacology, Regional Institute of Medical Sciences, Imphal, India


Objective: The study was aimed to evaluate and compare the anticonvulsant activity of aqueous leave extract of Portulaca oleracea Linn. and Eupatorium brimanicum DC in MES model in albino mice.

Methods: Aqueous Extracts were prepared by the soxhlet extraction method. MES model was chosen to evaluate anticonvulsant activity. 36 albino mice were selected and divided into 6 groups for this model. Group I received 2% gum acacia 1 ml/100 g orally. Group II received phenytoin-20 mg/kg orally. Group III and IV received 200 and 400 mg/kg of Portulaca oleracea Linn. Respectively. Group V and VI received 200 and 400 mg/kg of Eupatorium brimanicum DC respectively.

Results: The extracts didn’t show any toxicity and significantly reduced hind limb tonic extension (HLTE) duration in MES model (50 mA, 0.2 sec) at higher doses.

Conclusion: The results suggest Portulaca oleracea Linn. and Eupatorium brimanicum DC extract possess anticonvulsant activity and justify their use in folk medicine.

Keywords: MES, Anticonvulsant, Portulaca oleracea Linn., Eupatorium brimanicum DC, Albino mice


1. Kaur H, Kumar B, Medhi B. Antiepileptic drugs in developmentpipeline: a recent update. Neurol Sci 2016;4:42-51.
2. Rang HP, Dale MM, Ritter JM, Flower RJ. editors. Rang and dale’s pharmacology. 6th ed. New Delhi: Churchill Livingstone Elsevier; 2008.
3. Stafstrom CE, Carmant L. Seizures and epilepsy: an overview for neuroscientists. Cold Spring Harb Perspect Med 2015;5:1-18.
4. Denial HL. Seizures and epilepsies. In: Harrison’s principles of internal medicine. Kasper DL, Fauci AS, Hauser SL, Lango DL, Jameson JL, Loscalzo J. editors. 17th ed. New York: Mc Graw Hill Education; 2008. p. 2498-512.
5. Ngugi AK, Bottomely C, Kleinschmidt L. Estimation of the burden of active and lifetime epilepsy: a meta-analytic approach. Epilepsia 2010;51:883-90.
6. Sinha SC. Medicinal plant of Manipur, Imphal: Manipur association for science and society; 1996.
7. Deb DB. Compositae (Asteraceae). The flora of Tripura state. 1st ed. Vol. 2. New Delhi: Today and Tomorrow’s Printers and Publishers; 1983.
8. Verma SCL, Agarwal SL. Studies on Leptadenia reticulata part II-preliminary chemical investigations. Indian J Med Res 1962;50:439-45.
9. Kokate CK, Purohit AP, Gokhale SB. editors. Pharmacognosy. 45th ed. Pune: Nirali Prakashan; 2009.
10. Shah B, Seth AK. editors. Textbook of pharmacognosy and phytochemistry. 2nd ed. New Delhi: Elsevier; 2014.
11. OECD. OECD guidelines for testing of chemicals 423: Acute oral toxicity-Acute toxic class method. Paris; 2001. Available from: gl423.pdf. [Last accessed on 14 Sep 2018]
12. Castel Branco MM, Alves GL, Figueiredo IV, Falcao AC, Caramona MM. The maximal electroshock seizure (MES) model in the preclinical assessment of potential new antiepileptic drugs. Methods Find Exp Clin Pharmacol 2009;31:101-6.
13. Kulkarni SK. Practical pharmacology and clinical pharmacy. Delhi: Vallabh Publications; 2008.
14. Gupta SK. Drug screening methods. 3rd ed. New Delhi: Jaypee Brothers Medical Publishers (P) Ltd; 2016.
15. Remy M. "Why do medical researchers use mice?" Live Science. 2010. Available from: [Last accessed 10 Oct 2018]
16. Loscher W, Fassbender CP, Nolting B. The role of technical, biological and pharmacological factors in the laboratory evaluation of anti-convulsant drugs II, maximal electroshock seizure models. Epilepsy Res 1991;8:79-94.
17. Inan SY, Buyukafsar K. Antiepileptic effects of two Rho-kinase inhibitors, y-27632 and fasudin in mice. Br J Pharmcol 2008;155:44-51.
18. Bum EN, Nkantchoua GN, Njikam N, Taiwe GS, Ngoupaye GT, Pelanken MM. Anticonvulsant and sedative activity of leaves Senna spectabilis in mice. Int J Pharmacol 2010;6:123-8.
19. Xu X, Yu L, Chen G. Determination of flavanoids in Portulaca oleracea L. by capillary electrophosis with electrochemical detection. J Pharm Bio-Med Anal 2006;41:493-9.
20. Devi LR, Singh TS, Singh LW. Antifungal and phytochemical studies of Eupatorium brimanicum DC. Indian J Chem 2017;46:1868-72.
21. Paramdeep S, Damanpreet S, Rajesh KG. Phytoflavonoids: antiepileptics for the future. Int J Pharm Sci 2014;6:51-66.
32 Views | 32 Downloads
How to Cite
MEDHABATI, M., L. BABYCHA, A. BHATTACHARJEE, and N. GUNINDRO. “ANTICONVULSANT ACTIVITY OF PORTULACA OLERACEA LINN. AND EUPATORIUM BRIMANICUM DC IN MES INDUCED SEIZURE: A COMPARATIVE STUDY”. International Journal of Current Pharmaceutical Research, Vol. 13, no. 2, Mar. 2021, pp. 67-69, doi:10.22159/ijcpr.2021v13i2.41561.
Original Article(s)