DEVELOPMENT OF QUALITY CONTROL PARAMETERS FOR STANDARDIZATION OF A NOVELMUCILAGE OBTAINED FROM OKRA (ABELMOSCHUS ESCULENTUS (L.) MOENCH) FRUIT
Keywords:Mucilage, Okra, HPTLC, Quality control parameters
Objective: The main objective was to focus on qualitative and quantitative analysis of isolated okra mucilage by High-performance Thin Layer Chromatography to set up the quality control parameters for the isolated mucilage.
Methods: High-performance thin-layer chromatography (HPTLC) were applied for the identification of components present in methanolic and ethanolic fruit extract of okra at 254 nm and 356 nm. Quantitative analysis of amino acid ascorbic acid and total polyphenol content was determined.
Results: The results showed that the yield percentage for methanolic and ethanolic fruit extract of the okra fruit mucilage was found to be 13.5 and12.5% respectively. HPTLC determination of methanolic and ethanolic okra fruit extract showed the presence of 8 components with Rf values in the range of 0.14 to 0.62 and 0.14 to 0.54 respectively when detected at wavelengths 254 nm and at 356 nm. The total amino acid in okra fruit methanolic extract was found to be 11.45%w/w. The ascorbic acid content in methanolic okra fruit extract and ethanolic okra fruit extract was found to be 0.24 %w/w and 0.18% w/w respectively. The total phenolic contents (tannic acid equivalents, mg/g) in the methanolic and ethanolic okra fruit extracts were calculated to be 4.6 % w/wand 5.3% w/w respectively.
Conclusion: The data revealed dual benefits like it can act as a potential novel functional ingredient with health-promoting application due to the presence of ascorbic acid and total phenolics contents and at the same time the data provided guidelines for quality control parameters for the isolated okra fruit
2. Raymond CR, Paul JS, Sian CO. Handbook of pharmaceutical excipients. 5th ed. London (UK): The Pharmaceutical Press; 2006.
3. GT Kulkarni, K Gowthamarajan, RR Dhobe, F Yohanan, B Suresh. Development of controlled release spheriods using natural polysaccharide as release modifier. Drug Delivery 2005;12:201.
4. H Hamman, J Steenekamp. Use of natural gums and mucilages as pharmaceutical excipients. Curr Pharma 2015;21:4775-97.
5. Prajapati, GK Jani, NG Moradiya, NP Randeria. Pharmaceutical applications of various natural gums, mucilages and their modified forms. Carbohydr Polym 2013;92:1685-99.
6. Thakur M, Bhargava S, Praznik W, Loeppert R, Dixit VK. Effect of chlorophytumborivilianum santapau and fernandes on sexual dysfunction inhyperglycemic male rats. Chinese J Integrative Med 2009;15:448–53.
7. Dominguez Lopez A. Use of the fruit and stems of the prickly
pearcactus (Opuntia spp.) into human food. Food Sci Technol Int 1995;1:65–74.
8. R Malviya. Extraction characterization and evaluation of selected mucilage as pharmaceutical excipient. Polim Med 2011;41:39–44.
9. Kalu VD, Odeniyi MA, Jaiyeoba KT. Matrix properties of a new plant gum in controlled drug delivery. Arch Pharm Res 2007;30:884–9.
10. Nasipuri RN, Igwilo CI, Brown SA, Kunle OO. Mucilage from Abelmoschus esculentus fruits-a potential pharmaceutical raw material. J Pharm Res Dev 1996;1:22–28.
11. Dharani B, Sumathi S, Sivaprabha J. In vitro antioxidant potential of prosopis cineraria leaves. J Nat Prod Plant Resour 2011;1:26e32.
12. N Tavakoli, R Teimouri, H Hamishehkar. Characterization and evaluation of Okra gum as a tablet binder. Jundushapur J Nat Pharm Prod 2007;3:33–8.
13. Ameena K, Dilip C, Saraswathi R, Krishnan PN, Sankar C, Simi SP. Isolation of the mucilages from Hibiscus rosa sinensis linn. and Okra (Abelmoschus esculentus Linn.) and studies of the binding effects of the mucilages. Asian Pac J Trop Med 2010;3:539 43.
14. Manjule DB, Gazi S, Surwase U, Bhalchandra K. Isolation and characterization of Hibiscus rosa sinensis linn. (shoe flowers plant). Int J Pharm Chem Sci 2012;1:942 7.
15. Ohwoavworhua FO, Adelakun TA. Some physical characteristics of microcrystalline cellulose obtained from raw cotton of Cochlospermumplanchonii. Trop J Pharm Res 2005;4:1–7.
16. Khandelwal KR. Practical pharmacognosy techniques and experiments. 15th ed. Nirali Prakashan; 2008. p. 29.
17. Tyler VE, Brady LR, Robbers JE. Pharmacognosy Lea and Febiger, Philadelphia; 1976. p. 24.
18. Bhat V, Nayak R, Praveena MB, Cox PA, MJ Balick. The ethnobotanical approach to drug discovery. Sci Am 1994;270:82-7.
19. GT Kulkarni, K Gowthamarajan, RR Dhobe, F Yohanan, B Suresh. Development of controlled release spheriods using natural polysaccharide as release modifier. Drug Delivery 2005;12:201.
20. Whistler RL. Exudate gums. In: Whistler RL, Bemiller JN. editors. Industrial Gums: Polysaccharides and their derivatives. San Diego, Academic Press; 1993. p. 318-37.
21. Puttewar TY, Kshirsagar MD, Chandewar AV, Chikhale RV. Formulation and evaluation of or dispersible tablet of taste masked doxylamine succinate using ion exchange resin. J King Saud Univ Sci 2010;22:229 40.
22. Joshi Y, Chaudhary RK, Teotia UV. Formulation and evaluation of diclofenac sodium sustained release matrix tablets using Aegle marmelosgum. Int J Curr Trends Pharm Res 2013;1:174.