IN VITRO EVALUATION OF ANTHELMINTIC ACTIVITY OF ETHANOLIC EXTRACT OF CENTELLA ASIATICA LINN. IN INDIAN ADULT EARTHWORMS
Keywords:Helminthiasis, Centella asiatica Linn., Pheretima posthuma, Albendazole
Objective: Helminthiasis is one of the most prevalent parasitic infestations worldwide posing a major threat to public health. The control of these nematodes has relied largely on the use of a limited number of anthelmintics. However, emerging resistance and side effects to the currently available anthelmintic drugs is a major concern and discovery of newer anthelmintics with a novel mode of action is the need of the hour. The present study is aimed to evaluate the anthelmintic activity of ethanolic extract of Centella asiatica Linn. (EECA) on Indian earthworms (Pheretima posthuma).
Methods: The earthworms were divided into 4 groups with 6 worms in each group. The anthelmintic activity of EECA at two different concentrations (25 mg/ml and 50 mg/ml) was evaluated by assessing the time of paralysis and time of death of the worms. Albendazole was used as standard and 2% gum acacia as control.
Results: Albendazole at 25 mg/ml showed the highest anthelmintic activity and had significant difference (p<0.001) with EECA at both 25 mg/ml and 50 mg/ml.
Conclusion: Both doses of the test drug showed anthelmintic activity but the extract at either dose was found to be less effective than the standard drug. Further studies with higher doses of the extract should be done to evaluate the anthelmintic activity in a dose-dependent manner.
Soil-transmitted helminth infections. World Health Organisation. Available from: https://www.who.int/ Newsroom/Factsheets/Detail. [Last accessed on 31 Mar 2021]
Eliminating soil-transmitted helminthiases as a public health problem in children. World Health Organisation. Available from: https://www.who.int/neglected_diseases/resources. [Last accessed on 31 Mar 2021]
Dhaka R, Verma R, Kumar R, Chayal V, Bhalla K, Singh R, et al. Pattern and determinants of soil-transmitted helminthiasis in a rural area of Haryana: a school-based study. J Family Med Prim Care 2019;8:1971-5.
Chatterjee KD. Parasitology. 13th ed. New Delhi: CBS Publishers and Distributors Pvt. Ltd; 2015.
Bundy DA. Immunoepidemiology of intestinal helminthic infection I: The global burden of intestinal nematode disease. Trans Royal Soc Trop Med Hyg 1994;8:259-61.
E Madiba T, P Hadley G. Surgical management of worm volvulus. S Afr J Surg 1996;34:33-6.
Tripathi KD. Essentials of medical pharmacology. 8th ed. New Delhi: Jaypee Brother Medical Publishers (P) Ltd; 2019.
Keiser J, McCarthy J, Hotez P. Chemotherapy of helminth infections. In: Brunton LL, Hilal-dandan R, Knollmann BC. editors. Goodman and Gilman’s the pharmacological basis of therapeutics. 13th ed. New York: McGraw Hill Education; 2018. p. 1001-10.
Udavant PB, Satyanarayana SV, Upasani CD. In vitro anthelmintic activity of stems of Cuscuta reflexa. Int J Bioassays 2012;1:18-9.
Yadav P, Singh R. A review on anthelmintic drugs and their future scope. Int J Pharm Pharm Sci 2011;3:17-21.
Saranya S, Nair AV, Prathapan MP, Neethu AS, Kumar NS. Phytochemical analysis of Centella asiatica L. leaf extracts. Int J Adv Res 2017;5:1828-32.
Gohil KJ, Patel AJ, Gajjar AK. Pharmacological review on Centella asiatica: a potential herbal cure-all. Indian J Pharm Sci 2010;72:546-56.
Antony B, Santhakumari G, Merina B, Sheeba V, Mukkadan J. Hepatoprotective effect of Centella asiatica (L) in carbon tetrachloride-induced liver injury in rats. Indian J Pharm Sci 2006;68:772-6.
Krishnaiah D, Devi T, Bono A, Sarbatly R. Studies on phytochemical constituents of six Malaysian medicinal plants. J Med Plant Res 2009;3:67-72.
Knevel AM, Digangi FE. Jenkins’ quantitative pharmaceutical chemistry. 7th ed. New York: McGraw Hill Book Co; 1977.
Vidyarthi RD. A textbook of zoology. 14th ed. New Delhi: S Chand and Co; 1977.
Vigar Z. Atlas of medical parasitology. 2nd ed. Singapore: PG Publishing House; 1984.
Kokate CK. Practical pharmacognosy. New Delhi: Vallabh Prakashan; 1985.
Sinha SK, Mayanglambam M, Nongthombam S, Momin RG, Muralidas A, Paonam S. In vitro evaluation of anthelmintic activity of ethanolic extract of leaves of Toona ciliata M. Roem on Indian earthworm. Int J Adv Res 2019;7:250-3.
A Asase, AA Oteng Yeboah, GT Odamtten, MSJ Simmonds. Ethnobotanical study of some ghanaian antimalarial plants. J Ethnopharmacol 2005;99:1221-9.
S Markus, M Ernst. Medicinal plants in tropical countries: georg thieme verlag, Rudigerstrasse, Germany; 2005.
Kaur S, Kumar B, Puri S, Tiwari P, Divakar K. Comparative study of anthelmintic activity of aqueous and ethanolic extract of bark of Holoptelea integrifolia. Int J Drug Dev Res 2010;2:758-63.
Patel J, Kumar GS, Qureshi MS, Jena PK. Anthelmintic activity of ethanolic extract of whole plant of Eupatorium odoratum. Int J Phytomed 2010;2:127-32.
Wang GX, Han J, Zhao LW, Jiang DX, Liu YT, Liu XL. Anthelmintic activity of steroidal saponins from paris polyphylla. Phytomedicine 2010;17:1102-5.
Shekhawat N, Vijayvergia R. To study of anthelmintic activity of extracts of some medicinal plants. Int J Computat Sci Math 2011;3:183-7.
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