ANTIMICROBIAL AND ANTIDIARRHEAL EFFECTS OF FOUR CAMEROON MEDICINAL PLANTS: DICHROCEPHALA INTEGRIFOLIA, DIOSCOREA PREUSII, MELENIS MINUTIFLORA, AND TRICALYSIA OKELENSIS


Kamgang Rene, Fankem GaËtan Olivier, Ngo Tetka Jacqueline, Gonsu Kamga Hortense, Fonkouamarie Christine

Abstract


Objective: In order to verify the antidiarrheal activities of Dichrocephala integrifolia, Dioscorea preusii, Melenis minutiflora, Tricalysia okelensis, the in vitro antimicrobial effect on Salmonella typhi, Escherichia coli, Shigella dysenteriae A1 and Candida albicans, and the in vivo antidiarrheal activities on the intestine transit of the hydroethanol (v/v) plants extracts were studied.

Methods: The antimicrobial effect of the extracts was assayed in vitro by the disc diffusion and the agar dilution methods. For in vivo study, male and female mice received per os castor oil and one hour later different doses of the extracts.

Results: In vitro, D. integrifolia, D. preusii, M. minutiflora, and T. okelensis extract showed concentration-dependent activity against all the tested microbial strains with the inhibition zone ranged from 08 to 24 mm. D. integrifolia 0.5 mg/mL showed the lowest MIC on Candida albicans. The M. minutiflora and D. integrifolia MIC was 3 mg/mL on Escherichia coli and Shigella dysenteriae A1. In vivo, D. integrifolia, D. preusii, T. okelensis extract at 50 and 100 mg/kg bw and M. minutiflora 75 and 150 mg/kg bw significantly (P< 0.01) inhibited the intestinal charcoal transit. D. integrifolia 100 mg/kg bw exhibited the highest inhibition rate, 70%.

Conclusion: These results suggest that D. integrifolia, D. preusii, M. minutiflora and T. okelensis extracts possesses antimicrobial and antidiarrheal properties, could be effective for diarrhea treating, and could thus justify their use in traditional medicine to treat diarrhea. D. integrifolia could have the most efficiency antimicrobial properties.

Keywords: Dichrocephala integrifolia, Dioscorea preusii, Melenis minutiflora, Tricalysia okelensis, Antidiarrheal effects, Antimicrobial activity

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References


Semwal BC, Goyal A, Varshney V. Antidiarrheal activity of Rhododendron arborium leaves in wistar rats. Int J Pharm Res Bio-Sci 2014;3(3):591-600.

Brijesh S, Tetali P, Birdi TJ. Study of effect of anti-diarrheal medicinal plants on enteropathogenic Escherichia coli induced interleukin-8 secretion by intestinal epithelial cells. Altern Med Stud 2011;(1)e16:64-9.

Yakubu MT, Opakunle FK, Salimon SS, Ajiboye TO, Bamisaye FA, Quadri AL. Antidiarrheal activity of aqueous leaf extract of Ceratotheca sesamoides in rats. Bangladesh J Pharmacol 2012;7:14-20.

Akuodor GC, Muazzam I, Usman-Idris M, Megwas UA, Akpan JL. Evaluation of the antidiarrheal activity of methanol leaf extract of Bombax Buonopozense in rats. Ibnosina J Med Biomed Sci 2011;3(1):15-20.

Smith JA, van den Broek FAR, Martorel JCL, Hackbarth H, Ruksenas O, Zeller W. FELASA working grp and "principles and practice in ethical review of animal experiments across Europe: summary of the report of a FELASA Working Group on ethical evaluation of animal experiments." Lab Anim 2007;41(2):143-60.

Trease GE, Evans WC: Pharmacognosy, 12th ed. London: Baillere Tindall; 1983.

Sheela XQR, Ramani VA. Antimicrobial screening of polygonum barbatum leaf extract. J Curr Pharm Res 2011;3(3):117­8.

Akinyele TA, Okoh OO, Akinpelu DA, Okoh AI. In-Vitro antibacterial properties of crude aqueous and n-hexane extracts of the husk of Cocos nucifera. Mol 2011;16(3):2135-45.

Spencer ALR, Spencer JFT. Public Health Microbiology: Methods and protocols. New Jersey; Human Press Inc; 2004. p. 325-7.

Lakshminarayana M, Shivkumar H, Rimaben P, Bhargava VK. Antidiarrhoeal activity of leaf extract of Moringa Oleifera in experimentally induced diarrhoea in rats. Int J Phytomed 2011;3(2011):68-74.

Irimpan MT, Jolly CI, Sheela D. A Study of the phytochemical composition and antibacterial activity of Holostemma adakodien schultes. Int J Pharm Tech Res 2011;2(3):1208-10.

Lacmata ST, Kuete V, Dzoyem JP, Tankeo SB, Ngo Teke G, Kuiate JR, et al. Antibacterial activities of selected Cameroonian plants and their synergistic effects with antibiotics against bacteria expressing MDR phenotypes. Evidence-Based Complementary Altern Med 2012;1-11.

Murugesan S, Pannerselvam A, Tangavelou AC. Phytochemical screening and antimicrobial activity of the leaves of Memecylon umbellatum burm. F J Appl Pharm Sci 2011;01(01):42-5.

Rahman MA, Ahsna T, Islam S. Antibacterial and antifungal properties of methanol extract from the stem of Argyreia argentea. Bangaladesh J Pharmacol 2010;5:41-4.

Šiljegović ЈD, Stojković DS, Nikolić MM, Glamočlija JM, Soković MD. Antimicrobial activity of aqueous extract of Laetiporus sulphureus (bull.: fr.) murill. Nat Sci 2011;120:297-303.

Sridhar S, Deepa T, Elamathi R, Kavitha R, Kamalakannan, Suresh Kumar J. Antimicrobial activities of leaf extracts of Sapindus emarginatus Vahl. Int J Pharm Tech Res 2012;4(1):392-7.

Sunayana V, Vadivukkarasi A, Rajendran T, Francis X, Natarajan E. Antibacterial potential of Plectranthus amboinicus (Lour) Spreng. A study in vitro. J Swamy Bot Club 2003;20:55-8.

Haque A, Abdullah CS, Romana B, Rafique B, Zia-ul-Huda, Hossain SF, et al. Evaluation of anti-diarrheal and anti-diabetic activities of the stem, barks and leaves of the plant Vernonia cinerea (Family: Asteraceae). J Appl Pharm Sci 2013;3(01):069-072.

Capasso F, Mascolo N, Izzo AA, Gaginella TS. Dissociation of castor oil-induced diarrhea and intestinal mucosal injury in rat: effect of NGnitro-L-arginine methyl ester. Br J Pharmacol 1994;113(4):1127-30.

Anup M, Saikat D, Subhash CM. In vivo evaluation of antidiarrhoeal activity of the seed of Swietenia macrophylla King (Meliaceae). Trop J Pharm Res 2007;6(2):711-6.

Balogun SO, Tanayen JK, Ajayi AM, Ibrahim A, Ezeonwumelu JOC, Oyewale AA, et al. Preliminary evaluation of anti-diarrheal, ulcer-protective and acute toxicity of aqueous ethanolic stem bark extract of Ficus trichopoda in experimental rodents. Asian J Med Sci 2011;3(1):37-42.

Theoderau V, Floramont J, Hachet T, Bueno L. Absorptive and motor components of anti-diarrhoeal action of loperamide: an in vivo study in pigs. Gut 1991;32:1355-9.




About this article

Title

ANTIMICROBIAL AND ANTIDIARRHEAL EFFECTS OF FOUR CAMEROON MEDICINAL PLANTS: DICHROCEPHALA INTEGRIFOLIA, DIOSCOREA PREUSII, MELENIS MINUTIFLORA, AND TRICALYSIA OKELENSIS

Date

06-01-2015

Additional Links

Manuscript Submission

Journal

International Journal of Current Pharmaceutical Research
Vol 7, Issue 1, 2015 Page: 21-24

Online ISSN

0975-7066

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409 Views | 273 Downloads

Authors & Affiliations

Kamgang Rene
Laboratoryof Endocrinology and Radio-Isotopes, Institute of Medical Research and Medicinal Plants Studies (IMPM), Yaoundé, Cameroon

Fankem GaËtan Olivier
Animal Physiology Laboratory, Faculty of Science, University of Yaoundé I, Cameroon

Ngo Tetka Jacqueline
Animal Physiology Laboratory, Faculty of Science, University of Yaoundé I, Cameroon

Gonsu Kamga Hortense
Laboratory of Bacteriology of YUHC, Cameroon, 4Laboratory of Bacteriology, Centre Pasteur of Cameroon

Fonkouamarie Christine


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