PHYTOCHEMICAL ANALYSIS, ANTIOXIDANT AND ANTI MICROBIAL ACTIVITY OF WHITE & PINK PSIDIUM GUAJAVA LINNAEUS


Deepa Philip C, Indira Kumari R, Lavanya B

Abstract


Objective: The objective of the present study was to evaluate phytochemicals, antioxidant and anti microbial activity of white and pink (Guava) Psidium guajava Linnaeus.

Methods: Healthy and disease free fresh leaves and fruits of white and pink Psidium guajava Linnaeus were collected and extracted using ethanol and aqueous solvents and their contents were analysed for biological efficacy.

Results: The phytochemical analysis of extracts revealed the presence of phenols, glycosides, flavonoids and steroids. The leaf extracts of P. guajava determined by FRP (Ferric reducing power assay) method showed potent antioxidant activity. The antibacterial activity was analysed against five clinically significant organisms by disc diffusion method and leaf extracts of P. guajava Linn, showed maximum zone of inhibition against Pseudomonas aeruginosa, and Klebsiella pneumoniae. The most resistant bacteria were found to be Escherchia coli against P. guajava fruit extract that showed no activity.

Conclusion: This study reveals that guava leaf extracts exhibits better phytochemical, antioxidant and antibacterial activity than fruit extracts.

Keywords: Psidium guajava Linn, Phytochemicals, FRP, Disc diffusion.

| PDF | HTML |

References


Ammara H, Salma R, Farah D, Shahid M. Antimicrobial activity of some plant extracts having hepato protective effects. J Med Plants Res 2009;3(1):20-3.

Barbour E, Sharif MA, Sagherian VK, Harbe AN, Talkbouk RS, Tasheuk SN. Screening of selected indigenous plants of Lebanon for antimicrobial activity. J Ethnopharmacol 2004;93:1-7.

Yasunaka K, Abe F, Okabe H, Mumizi EE, Aguilavi A, Ryes-Chilps R. Antibacterial activity of crude extracts from Mexican medicinal plants and purified coumauries and vanthones. J Ethnopharmacol 2005;97:293-9.

Rabe T, Staden JV. Antibacterial activity of South African plants used for medicinal purposes. J Ethnopharmcol 1997;56:81-7.

Pathak RK, Ojha CM. Genetic resources of guava. Vol. I. Fruit Crops, Part 1, In; Advance in Horticulture. Malhotra Publishing House, New Delhi: 1993.

Bailey LH. The standard encyclopedia of horticulture. Vol. II. Macmillan Co, New York; 1960.

Nweze ET, Okafor JI, Njoku O. Antimicrobial activities of methanolic extract of Trumeguineesis (Schumm and Thorn) and Morinda lucinda Benth used in Nigerian herb medicinal practice. J Bio Res Biotechnol 2004;2(1):34-46.

Senthilkumar PK, Reetha D. Screening of antimicrobial properties of certain Indian medicinal plants. J Phytol 2009;1(3):193-8.

Bauer RW, Kirby MD, Sherris JC. Antibiotic susceptibility testing by standard single disc diffusion method. Am J Clin Pathol 1966;45:493-6.

John DT, James HJ. Antimicrobial susceptibility testing general considerations. Manual of Clinical Microbiology. 7 ed. Am Soc Microbiol Washington DC; 1999.

Kohner PC, Rosenblatt JE, Cockerill FR. Comparison of agar dilution, broth dilution, and disk diffusion testing of ampicillin against Haemophilus species by using in-house and commercially prepared media. J Clin Microbiol 1994;32(6):1594-6.

Mathabe MC, Nikolova RV, Lall N. Antibacterial activities of medicinal plants used for the treatment of diarrhoea in Limpopo Province. S Afr J Ethnopharmcol 2006;105(1-2):286-93.

Chu YH, Chang CL, Hsu HF. Flavonoid content of several vegetables and their antioxidant activity. J Sci Food Agric 2000;80(5):561-6.

Philip D, Kaleena PK, Valivittan K. Phytochemical screening and antimicrobial activity of Sansevieria roxburghiana Schult. & Schult. F. Middle-East J Sci Res 2011;10(4):512-8.

Vikrant A, Narender T, Kashyap. Preliminary phytochemical analysis of the extracts of psidium leaves. J Pharmacogn Phytochem 2012;1(1):1-5.

Farag RS, Daw ZY, Hewedi FM, El‐Baroti GSA. Antimicrobial activity of some Egyptian spice essential oils. J Food Prot 1989;52:665–7.

Gao X, Bjork L, Trajkovski V, Uggla M. Evalution of antioxidant activities of rosehip ethanol extract in different test system. J Agric Food Chem 2000;80:2021-7.




About this article

Title

PHYTOCHEMICAL ANALYSIS, ANTIOXIDANT AND ANTI MICROBIAL ACTIVITY OF WHITE & PINK PSIDIUM GUAJAVA LINNAEUS

Date

10-04-2015

Additional Links

Manuscript Submission

Journal

International Journal of Current Pharmaceutical Research
Vol 7, Issue 2, 2015 Page: 29-31

Online ISSN

0975-7066

Statistics

206 Views | 31 Downloads

Authors & Affiliations

Deepa Philip C
Head, PG Department of Biochemistry, Annai Violet College of Arts and Science (Affiliated to University of Madras) Ambattur, Chennai 600053, Tamil Nadu, India

Indira Kumari R

Lavanya B


Refbacks

  • There are currently no refbacks.