A COMPARATIVE STUDY ON IN VITRO ANTIOXIDANT AND ANTIBACTERIAL ACTIVITIES OF METHANOL EXTRACT FROM THE LEAVES OF STACHYTARPHETA INDICA (L) VAHL AND PREMNA CORYMBOSA ROTTL


Antony Rose Immaculate, V. Uma Rani

Abstract


Objective: The objective of this research was to investigate the in vitro antioxidant and antibacterial activities of methanol extract from the leaves of Stachytarpheta indica (L) vahl. and Premna corymbosa Rottl.

Methods: The various photochemicals were analyzed according to the methods described by Allen and Harbone. The total phenolic contents were estimated by Folin–Ciocalteu method. Antioxidant activities of these plants were tested on the basis of ABTS (2,2'-azino-bis-3-ethyl benzthiazoline-6-sulphonic acid) radical scavenging assay, inhibition of lipid peroxidation, Super oxide radical scavenging activity, Nitric oxide radical scavenging activity and Metal chelating activity. Similarly antibacterial activity was performed by Disc diffusion method against Staphylococcus aureus, Pseudomonas aeruginosa, Klebsiella pnemoniae, Escherichia coli and Enterococcus faecalis.

Results: The phytochemical analysis showed the presence of Tannins, Flavonoids, Alkaloids and Anthraquinones. The total phenolic contents were more in P. corymbosa (381.10±13.00 mg/g) than S. indica (195.72±8.75 mg/g). The methanol extract of the leaves of P. cormbosa showed significant antioxidant activity, while it was comparatively less in S. indica. Similarly the study on antibacterial activity of these two plant leaves extracts revealed inhibitory activity. However P. corymbosa showed higher inhibitory zone against E. coli, K. pneumonia, P. aeroginosa (18, 17, 16 mm) respectively compared with S. indica.

Conclusion: This research work has made it clear that P. corymbosa possess excellent antibacterial and antioxidant activity and the extracts can be more widely used in developing countries for the prevention and treatment of ageing related diseases and may be considered as good source for drug discovery.

Keywords: Stachytarpheta indica, Premna corymbosa, Antioxidant, Antibacterial.

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About this article

Title

A COMPARATIVE STUDY ON IN VITRO ANTIOXIDANT AND ANTIBACTERIAL ACTIVITIES OF METHANOL EXTRACT FROM THE LEAVES OF STACHYTARPHETA INDICA (L) VAHL AND PREMNA CORYMBOSA ROTTL

Date

17-10-2015

Additional Links

Manuscript Submission

Journal

International Journal of Current Pharmaceutical Research
Vol 7, Issue 4, 2015 Page: 43-48

Online ISSN

0975-7066

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267 Views | 21 Downloads

Authors & Affiliations

Antony Rose Immaculate
Department of Plant Biology and Plant Biotechnology, Guru Nanak College, Velachery, Chennai, Tamil Nadu, India
India

V. Uma Rani


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