Komalavalli T., Packia Lincy M., Muthukumarasamy S., Mohan V. R.


Objective: The ethanol extract of Sonerila tinnevelliensis Fischer whole plant was investigated for its antidiabetic and antihyperlipidaemic effect in Wistar Albino rats.

Methods: Diabetes was induced in Albino rats by administration of alloxan monohydrate (150 mg/kg, i. p). The ethanol extracts of S. tinnevelliensis at a dose of 200 and 400 mg/kg of body weight were administered at single dose per day to diabetes induced rats for a period of 14 d. The effect of ethanol extract of S. tinnevelliensis whole plant extract on blood glucose, plasma insulin, creatinine, urea, glycosylated haemoglobin, serum lipid profile [total cholesterol (TC), triglycerides (TG), low density lipoprotein–cholesterol (LDL-C), very low density lipoprotein–cholesterol (VLDL-C), high density lipoprotein–cholesterol (HDL-C) and phospholipid (PL)] serum protein, albumin, globulin, serum enzymes [serum glutamate pyruvate transaminases (SGPT), serum glutamate oxaloacetate transaminases (SGOT), and alkaline phosphatase (ALP)] were measured in the diabetic rats.

Results: The ethanol extract of S. tinnevelliensis whole plant elicited significant reductions of blood glucose (p<0.05), lipid parameters except HDL-C, serum enzymes and significantly increased HDL-C. The extracts also caused significant increase in plasma insulin (p<0.05) in the diabetic rats.

Conclusion: The ethanol extracts of S. tinnevelliensis whole plant possesses significant antidiabetic and antihyperlipidaemic effects in alloxan induced diabetic rats.

Keywords: Sonerila tinnevelliensis, Diabetes mellitus, Alloxan, Hyperglycemia.

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Diabetes Mellitus: Report of a WHO Study Group, World Health Organ. Tech Rep Ser 1985;727:1-113.

Mayfield J. Diagnosis and classification of diabetes mellitus: new criteria. Am Fam Physician 1998;58:1355.

Li WL, Zheng HC, Bukuru J, De Kimpe N. Natural medicines used in the traditional Chinese medical system for therapy of diabetes mellitus. J Ethnopharmacol 2004;92:1–21.

Satyanarayana T, Katyayani BM, Latha HE, Mathews AA, Chinna EM. Hypoglycemic and antihyperglycemic effect of alcoholic extract of Euphorbia leucophylla and its fractions in normal and in alloxan induced diabetic rats. Pharmacogn Mag 2006;2:244.

Kamtchouing P, Sokeng DS, Moundipa PF, Pierre W, Jatsa BH, Lontsi D. Protective role of Anacardium occidentale extract against streptozotocin-induced diabetes in rats. J Ethnopharmacol 1998;62:95-9.

Alarcon-Aguilara FJ, Roman-Ramos R, Perez-Gutierrez S, Aguilar-Contreras A, Contreras-Weber CC, et al. Study of the anti-hyperglycemic effect of plants used as antidiabetics. J Ethnopharmacol 1998;61:101-10.

Rajendran A, Ravikumar, Henry AN. Plant genetic resources and knowledge of traditional medicine in Tamil Nadu. Ancient Sci Life 2000;20:25-8.

Lalitha rani S, Kalpana Devi V, Tresina Soris P, Maruthu Pandian A, Mohan VR. Ethnomedicinal plants used by kanikkars of agastiarmalai biosphere reserve, western ghats. J Ectobiotechnol 2011;3:16-25.

Sutha S, Mohan VR, Kumaresan S, Murugan C, Athiperumalsami T. Ethnomedicinal plants used by the tribals of Kalakad-Mundanthurai tiger reserve (KMTR) Western Ghats, Tamil Nadu for the treatment of rheumatism. Indian J Trad Knowled 2010;9:502-9.

OECD. Organisation for economic co-operation and Development). OECD guidelines for the testing of chemicals/Section 4: Health Effects Test No. 423; Acute oral Toxicity-Acute Toxic Class method. OECD. Paris; 2002.

Nagappa AN, Thakurdesai PA, Venkat Rao N, Sing J. Antidiabetic activity of Terminalia catappa Linn. fruits. J Ethnopharmacol 2003;88:45-50.

Sasaki T, Masty S, Sonae A. Effect of acetic acid concentration on the colour reaction in the otoluidine boric acid method for blood glucose estimation. Rinshbo Kagaku 1972;1:346-53.

Anderson L, Dinesen B, Jorgonsen PN, Poulsen F, Roder ME. Enzyme immune assay for intact human insulin in serum or plasma. Clin Chem 1993;39:578-82.

Varley H. Practical clinical biochemistry, Arnold Heinemann Publication Pvt. Ltd.; 1976. p. 452.

Owen JA, Iggo JB, Scangrett FJ, Steward, IP. Determination of creatinine in plasma serum, a critical examination. J Biochem 1954;58:426-37.

Karunanayake EH, Chandrasekharan NV. An evaluation of a colorimetric procedure for the estimation of glycosylated haemoglobin and establishment of reference values for sri lanka. J Natl Sci Counc 1985;13:235-58.

Parekh AC, Jung. Cholesterol determination with ferric acetate, uranium acetate and sulphuric acid, ferrous sulphate reagent. Anal Chem 1970;112:1423-7.

Rice EW. Triglycerides in serum. In: Standard Methods. Clinical Chemistry. 9ed Roderick MP, Academic press, New York; 1970. p. 215-22.

Friedwald WT, Levy RI, Fredrickson DS. Estimation of the concentration of low density lipoprotein cholesterol in plasma, without use of the preparative ultra centrifuge. Clin Chem 1972;18:499-502.

Warnick GR, Nguyan T, Albers AA. Comparison of improved precipitation methods for quantification of high density lipoprotein cholesterol. Clin Chem 1985;31:217.

Takayama M, Itoh S, Nagasaki T, Tanimizu I. A new enzymatic method for determination of serum phospholipids. Clin Chem Acta 1977;79:93–8.

Lowry OH, Rosenbrough NJ, Farr AL, Randall RJ. Protein measurement with the folin’s phenol reagent. J Biol Chem 1951;193:265-75.

Reitman S, Frankel SA. Colorimetric method for the determination of serum glutamic oxaloacetic and glutamic pyruvic transaminases. Am J Clin Pathol 1957;28:56-63.

King EJ, Armstrong AR. Determination of serum and bile phosphatase activity. Cannad Med Assoc J 1934;31:56-63.

Rajiv Gandhi G, Sasikumar P. Antidiabetic effect of Merremia emarginata Burm. F. in Streptozotocin induced diabetic rats. Asian Pacific J Trop Biomed 2012;2:281-6.

Szkudelski T. The mechanism of alloxan and streptozotocin action in B cells of the rat pancreas. Physiol Res 2001;50:536-46.

Nammi S, Boini MK, Lodagala SD, Behara RB. The juice of fresh leaves of Catharanthus roseus Linn. reduces blood glucose in normal and alloxan diabetic rabbits. BMC Complement Altern Med 2003;3:4.

Veeramani C, Pushpavalli G, Pugalendi KV. Antihyperglycaemic effect of Cardiospermum halicacabum Linn. leaf extract on streptozotocin induced diabetic rats. J Appl Biomed 2008;6:19-26.

Sezik E, Aslan M, Yesilada E, Ito S. Hypoglycaemic activity of Gentiana olivieri and isolation of the active constituent through bioassay-directed fractionation techniques. Life Sci 2005;76:1223-38.

Maruthupandian A, Mohan VR. Antidiabetic, antihyperlipidaemic and antioxidant activity of Pterocarpus marsupian Roxb. In alloxan induced diabetic rats. Int J Pharm Technol 2011;3:1681-7.

Shanmugasundaram R, Devi VK, Soris PT, Maruthupandian A, Mohan VR. Antidiabetic, antihyperlipidaemic and antioxidant activity of Senna auriculata (L.) Roxb. leaves in alloxan induced diabetic rats. Int J Pharm Tech Res 2011;3:747-56.

Kala SM, Tresina PS, Mohan VR. Antioxidant, antihyperlipidaemic and antidiabetic activity of Eugenia singamattina Bedd. Leaves in alloxan induced diabetic rats. Int J Pharm Pharm Sci 2012a;4:412-6.

Kala SM, Tresina PS, Mohan VR. Antioxidant antihyperlipidaemic and antidiabetic activity of Eugenia floccosa Bedd. leaves in alloxan induced diabetic rats. J Basic Clini Pharm 2012b;3:235-40.

Shajeela PS, Kalpanadevi V, Mohan VR. Potential antidiabetic, hypolipidaemic and antioxidant effects of Nymphaea pubescens extract in alloxan induced diabetic rats. J Appl Pharm Sci 2012;2:83-8.

Lal SS, Sukla Y, Singh A, Andriyas EA, Lall AM. Hyperuricemia, high serum urea and hypoproteinemia are are the risk factor for diabetes. Asian J Med Sci 2009;1:33-4.

Deguchi Y, Miyazaki K. Anti-hyperglycemic and antihyperlipidemic effects of guava leaf extract. Nutr Metab 2010;7:9.

Alagammal M, Nishanthini A, Mohan VR. Antihyperglycemic and antihyperlipidaemic effect of Polygala rosmarinifolia Wright and Arn on alloxan induced diabetic rats. J Appl Pharm Sci 2012;2:143-8.

Lanjhiyana S, Garabadu D, Ahirwar D, Bigoniya P, Rana AC, Patra KC, et al. Antidiabetic activity of methanolic extract of stem bark of Elaeodendron glaucum Pers. in alloxanized rat model. Adv Appl Sci Res 2011;2:47-62.

Stanely P, Prince M, Menon V. Hypoglycemic and other related actions of Tinospora cordifolia roots in alloxan induced diabetic rats. J Ethnopharmacol 1999;70:9-15.

Hwang HJ, Kim SW, Lim JM, Joo JH, Kim HO, Kim HM, Yun JW. Hypoglycemic effect of crude epoxy polysaccharides produced by a medicinal mushroom Phellinus baumii in streptozotocin induced diabetic rats. Life Sci 2005;76:3069-80.

Preethi KC, Kuttan R. Hepato and reno protective action of Calendula officinalis L. flower extract. Indian J Exp Biol 2009;47:163-8.

Rajalingam R, Srinivasan N, Govindarajulu P. Effect of alloxan induced diabetes on lipid profiles in renal cortex and medulla of mature albino rats. Indian J Exp Biol 1993;31:577-9.

Pathak RM, Ansai S, Mahmood A. Changes in chemical composition of intestinal brush border membrane in alloxan induced chronic diabetes. Indian J Exp Biol 1981;19:503-5.

Cohn RM, Roth KS. Lipid and lipoprotein metabolism In: Biochemistry and Diseases, Williams and Willkins publishers, Baltimore; 1996. p. 280.

Venkateswaran S, Pari L, Saravanan G. Effect of Phaseolus vulgaris on circulatory antioxidants and lipids in streptozotocininduced diabetic rats. J Med Food 2002;5:97–104.

Pari L, Satheesh AM. Antidiabetic effect of Boerhavia diffusa: effect on serum and tissue lipids in experimental diabetes. J Med Food 2004;7:472–47.

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International Journal of Current Pharmaceutical Research
Vol 7, Issue 4, 2015 Page: 53-57

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Authors & Affiliations

Komalavalli T.

Packia Lincy M.

Muthukumarasamy S.

Mohan V. R.
3Department of Botany, Sri K. G. S. Arts College, Srivaikuntam, Tamil Nadu, India


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